Abstract
Specific method for direct radioimmunoassay of IRP and IRI separately in human plasma has been described. The method is used for extraction of total insulin and separation of IRP from IRI by paper chromatography to be assayed separately. The separation of the two components is identified and confirmed by column chromatography, paper chromatography and ultraviolet spectral analysis in comparison with the standard compounds. 134 plasma samples of different cases were investigated for the determination of IRI, IRP and IRT. Out of these 39 were normals, 16 normal obes, 21 juvinil diabetes, 18 overt adult diabetes, 10 recent adult diabetes, 12 hypothyroidism and 18 bilharzial hepatosplenomegaly. They were used to evaluate the test levels in comparison with blood sugar concentration.
Megahed, Y M;
Abdel-Wahab, M F;
El-Shawarbie, K;
Sadek, S;
Amer, M S;
[1]
Ain Shams Univ., Cairo (Egypt). Faculty of Medicine)
- Atomic Energy Establishment, Cairo (Egypt). Radioisotope Department
Citation Formats
Megahed, Y M, Abdel-Wahab, M F, El-Shawarbie, K, Sadek, S, Amer, M S, and Ain Shams Univ., Cairo (Egypt). Faculty of Medicine).
Direct radioimmunoassay of proinsulin and insulin in human plasma by the chromatographic technique.
Germany: N. p.,
1976.
Web.
Megahed, Y M, Abdel-Wahab, M F, El-Shawarbie, K, Sadek, S, Amer, M S, & Ain Shams Univ., Cairo (Egypt). Faculty of Medicine).
Direct radioimmunoassay of proinsulin and insulin in human plasma by the chromatographic technique.
Germany.
Megahed, Y M, Abdel-Wahab, M F, El-Shawarbie, K, Sadek, S, Amer, M S, and Ain Shams Univ., Cairo (Egypt). Faculty of Medicine).
1976.
"Direct radioimmunoassay of proinsulin and insulin in human plasma by the chromatographic technique."
Germany.
@misc{etde_7124653,
title = {Direct radioimmunoassay of proinsulin and insulin in human plasma by the chromatographic technique}
author = {Megahed, Y M, Abdel-Wahab, M F, El-Shawarbie, K, Sadek, S, Amer, M S, and Ain Shams Univ., Cairo (Egypt). Faculty of Medicine)}
abstractNote = {Specific method for direct radioimmunoassay of IRP and IRI separately in human plasma has been described. The method is used for extraction of total insulin and separation of IRP from IRI by paper chromatography to be assayed separately. The separation of the two components is identified and confirmed by column chromatography, paper chromatography and ultraviolet spectral analysis in comparison with the standard compounds. 134 plasma samples of different cases were investigated for the determination of IRI, IRP and IRT. Out of these 39 were normals, 16 normal obes, 21 juvinil diabetes, 18 overt adult diabetes, 10 recent adult diabetes, 12 hypothyroidism and 18 bilharzial hepatosplenomegaly. They were used to evaluate the test levels in comparison with blood sugar concentration.}
journal = []
volume = {12:4}
journal type = {AC}
place = {Germany}
year = {1976}
month = {Apr}
}
title = {Direct radioimmunoassay of proinsulin and insulin in human plasma by the chromatographic technique}
author = {Megahed, Y M, Abdel-Wahab, M F, El-Shawarbie, K, Sadek, S, Amer, M S, and Ain Shams Univ., Cairo (Egypt). Faculty of Medicine)}
abstractNote = {Specific method for direct radioimmunoassay of IRP and IRI separately in human plasma has been described. The method is used for extraction of total insulin and separation of IRP from IRI by paper chromatography to be assayed separately. The separation of the two components is identified and confirmed by column chromatography, paper chromatography and ultraviolet spectral analysis in comparison with the standard compounds. 134 plasma samples of different cases were investigated for the determination of IRI, IRP and IRT. Out of these 39 were normals, 16 normal obes, 21 juvinil diabetes, 18 overt adult diabetes, 10 recent adult diabetes, 12 hypothyroidism and 18 bilharzial hepatosplenomegaly. They were used to evaluate the test levels in comparison with blood sugar concentration.}
journal = []
volume = {12:4}
journal type = {AC}
place = {Germany}
year = {1976}
month = {Apr}
}