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Ultra-fast repair of single-strand breaks in DNA of. gamma. -irradiated Chinese hamster cells

Abstract

Studies of the effect of thermal treatment of Chinese hamster cells on sedimentation of DNA in the alkaline sucrose gradient showed that heating the cells to 68/sup 0/C for 15 min caused the same degradation as ..gamma..-irradiation with 5 to 7 krad at 37/sup 0/C. The inhibition of cellular repair enzymes by heating was therefore unacceptable. The process of ultra-fast repair is essentially determined by the DNA-ligase reaction, which is activated in the presence of Mg ions, and inhibited in mammalian cells in the presence of EDTA and pyrophosphate. Sedimentation profiles were therefore measured for the DNA of Chinese hamster cells ..gamma..-irradiated (5 krad) at 0/sup 0/C or 22/sup 0/C in the presence of Mg/sup + +/, or EDTA and pyrophosphate, and the results demonstrated ultra-fast repair only at 20 to 37/sup 0/C, in contrast to bacteria. A study was made of the temperature dependence of the activity of the DNA ligases isolated from E.coli and rabbit bone marrow. The NAD-dependent bacterial DNA ligase was active at temperatures from 0 to 40/sup 0/C, whereas ATP-dependent DNA ligase of mammals only showed activity in the range 15 to 40/sup 0/C. The differing temperature dependences of ultra-fast repair in bacterial and mammalian  More>>
Authors:
Leontjeva, G A; Mantzighin, Yu A; Gaziev, A I [1] 
  1. AN SSSR, Pushchino-na-Oke. Inst. Biologicheskoj Fiziki
Publication Date:
Dec 01, 1976
Product Type:
Journal Article
Reference Number:
AIX-08-298838; EDB-77-064162
Resource Relation:
Journal Name: Int. J. Radiat. Biol.; (United Kingdom); Journal Volume: 30:6
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DNA; STRAND BREAKS; BIOLOGICAL REPAIR; ANIMAL CELLS; CATALYSIS; CHEMICAL RADIATION EFFECTS; COMPARATIVE EVALUATIONS; EDTA; ESCHERICHIA COLI; GAMMA RADIATION; IN VITRO; LIGASES; MAGNESIUM COMPOUNDS; METABOLISM; PYROPHOSPHATES; RESPONSE MODIFYING FACTORS; TEMPERATURE DEPENDENCE; TIME DEPENDENCE; ALKALINE EARTH METAL COMPOUNDS; AMINO ACIDS; BACTERIA; BIOLOGICAL RECOVERY; CARBOXYLIC ACIDS; CHELATING AGENTS; CHEMISTRY; ELECTROMAGNETIC RADIATION; ENZYMES; IONIZING RADIATIONS; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; OXYGEN COMPOUNDS; PHOSPHORUS COMPOUNDS; RADIATION CHEMISTRY; RADIATION EFFECTS; RADIATIONS; RECOVERY; REPAIR; 560121* - Radiation Effects on Cells- External Source- (-1987)
OSTI ID:
7122332
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: IJRBA
Submitting Site:
INIS
Size:
Pages: 577-580
Announcement Date:
Apr 01, 1977

Citation Formats

Leontjeva, G A, Mantzighin, Yu A, and Gaziev, A I. Ultra-fast repair of single-strand breaks in DNA of. gamma. -irradiated Chinese hamster cells. United Kingdom: N. p., 1976. Web. doi:10.1080/09553007614551451.
Leontjeva, G A, Mantzighin, Yu A, & Gaziev, A I. Ultra-fast repair of single-strand breaks in DNA of. gamma. -irradiated Chinese hamster cells. United Kingdom. doi:10.1080/09553007614551451.
Leontjeva, G A, Mantzighin, Yu A, and Gaziev, A I. 1976. "Ultra-fast repair of single-strand breaks in DNA of. gamma. -irradiated Chinese hamster cells." United Kingdom. doi:10.1080/09553007614551451. https://www.osti.gov/servlets/purl/10.1080/09553007614551451.
@misc{etde_7122332,
title = {Ultra-fast repair of single-strand breaks in DNA of. gamma. -irradiated Chinese hamster cells}
author = {Leontjeva, G A, Mantzighin, Yu A, and Gaziev, A I}
abstractNote = {Studies of the effect of thermal treatment of Chinese hamster cells on sedimentation of DNA in the alkaline sucrose gradient showed that heating the cells to 68/sup 0/C for 15 min caused the same degradation as ..gamma..-irradiation with 5 to 7 krad at 37/sup 0/C. The inhibition of cellular repair enzymes by heating was therefore unacceptable. The process of ultra-fast repair is essentially determined by the DNA-ligase reaction, which is activated in the presence of Mg ions, and inhibited in mammalian cells in the presence of EDTA and pyrophosphate. Sedimentation profiles were therefore measured for the DNA of Chinese hamster cells ..gamma..-irradiated (5 krad) at 0/sup 0/C or 22/sup 0/C in the presence of Mg/sup + +/, or EDTA and pyrophosphate, and the results demonstrated ultra-fast repair only at 20 to 37/sup 0/C, in contrast to bacteria. A study was made of the temperature dependence of the activity of the DNA ligases isolated from E.coli and rabbit bone marrow. The NAD-dependent bacterial DNA ligase was active at temperatures from 0 to 40/sup 0/C, whereas ATP-dependent DNA ligase of mammals only showed activity in the range 15 to 40/sup 0/C. The differing temperature dependences of ultra-fast repair in bacterial and mammalian cells are in agreement with the temperature dependences of the activities of isolated enzymes, and the results suggest that the process of ultra-fast repair of single-strand breaks of DNA takes place in both bacterial and mammalian cells.}
doi = {10.1080/09553007614551451}
journal = {Int. J. Radiat. Biol.; (United Kingdom)}
volume = {30:6}
journal type = {AC}
place = {United Kingdom}
year = {1976}
month = {Dec}
}