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Protection of Escherichia coli cells against the lethal effects of ultraviolet and X-irradiation by prior X-irradiation. A genetic and physiological study

Journal Article:

Abstract

When log phase cells of wild-type E.coli K-12 were maintained in growth medium after X-irradiation, they became progressively more resistant to a subsequent exposure to UV or X-radiation. The time to achieve maximum resistance was about 60 min. The uvrB, uvrD, polA and certain exrA strains (W3110 background) also demonstrated this X-ray-induced resistance to subsequent UV or X-irradiation but recA, recB, lex (AB1157 or W3110 backgrounds) and other exrA strains (AB1157 background) did not. The resistance induced in wild-type, uvrB and uvrD cells was characterized by the production or enhancement of a shoulder on the survival curves obtained for the second irradiation, while the resistance induced in the W3110 exrA strains was expressed only as a change in slope. The induction of resistance in the W3110 exrA strain was not inhibited by the presence of chloramphenical, but that in the wild-type cells appeared to be. The production or enhancement of a shoulder on the survival curves of the rec/sup +/ lex/sup +/ exr/sup +/ cells is consistent with the concept of the radiation induction of repair enzymes. Alternative explanations, however, are discussed.
Authors:
Smith, K C; Martignoni, K D [1] 
  1. Stanford Univ., Calif. (USA). Dept. of Radiology
Publication Date:
Dec 01, 1976
Product Type:
Journal Article
Reference Number:
AIX-08-298873; EDB-77-064201
Resource Relation:
Journal Name: Photochem. Photobiol.; (United Kingdom); Journal Volume: 24:6
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ESCHERICHIA COLI; RADIOSENSITIVITY; X RADIATION; RADIOSENSITIVITY EFFECTS; BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL REPAIR; CHLORAMPHENICOL; COMPARATIVE EVALUATIONS; FRACTIONATED IRRADIATION; MUTANTS; PHOTOSENSITIVITY; REACTION KINETICS; RESPONSE MODIFYING FACTORS; SURVIVAL CURVES; SYNERGISM; TEMPORAL DOSE DISTRIBUTIONS; ULTRAVIOLET RADIATION; ANTIBIOTICS; BACTERIA; BIOLOGICAL EFFECTS; BIOLOGICAL RECOVERY; DRUGS; ELECTROMAGNETIC RADIATION; IONIZING RADIATIONS; IRRADIATION; KINETICS; MICROORGANISMS; RADIATION DOSE DISTRIBUTIONS; RADIATION EFFECTS; RADIATIONS; RECOVERY; REPAIR; SENSITIVITY; 560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)
OSTI ID:
7122321
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: PHCBA
Submitting Site:
INIS
Size:
Pages: 515-523
Announcement Date:
Apr 01, 1977

Journal Article:

Citation Formats

Smith, K C, and Martignoni, K D. Protection of Escherichia coli cells against the lethal effects of ultraviolet and X-irradiation by prior X-irradiation. A genetic and physiological study. United Kingdom: N. p., 1976. Web.
Smith, K C, & Martignoni, K D. Protection of Escherichia coli cells against the lethal effects of ultraviolet and X-irradiation by prior X-irradiation. A genetic and physiological study. United Kingdom.
Smith, K C, and Martignoni, K D. 1976. "Protection of Escherichia coli cells against the lethal effects of ultraviolet and X-irradiation by prior X-irradiation. A genetic and physiological study." United Kingdom.
@misc{etde_7122321,
title = {Protection of Escherichia coli cells against the lethal effects of ultraviolet and X-irradiation by prior X-irradiation. A genetic and physiological study}
author = {Smith, K C, and Martignoni, K D}
abstractNote = {When log phase cells of wild-type E.coli K-12 were maintained in growth medium after X-irradiation, they became progressively more resistant to a subsequent exposure to UV or X-radiation. The time to achieve maximum resistance was about 60 min. The uvrB, uvrD, polA and certain exrA strains (W3110 background) also demonstrated this X-ray-induced resistance to subsequent UV or X-irradiation but recA, recB, lex (AB1157 or W3110 backgrounds) and other exrA strains (AB1157 background) did not. The resistance induced in wild-type, uvrB and uvrD cells was characterized by the production or enhancement of a shoulder on the survival curves obtained for the second irradiation, while the resistance induced in the W3110 exrA strains was expressed only as a change in slope. The induction of resistance in the W3110 exrA strain was not inhibited by the presence of chloramphenical, but that in the wild-type cells appeared to be. The production or enhancement of a shoulder on the survival curves of the rec/sup +/ lex/sup +/ exr/sup +/ cells is consistent with the concept of the radiation induction of repair enzymes. Alternative explanations, however, are discussed.}
journal = {Photochem. Photobiol.; (United Kingdom)}
volume = {24:6}
journal type = {AC}
place = {United Kingdom}
year = {1976}
month = {Dec}
}