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Thermosensitive mutant of Bacillus subtilis deficient in uracil and cell division

Journal Article:

Abstract

Thermonsensitive division mutants were derived from Bacillus subtilis Marburg 168 thy trp/sub 2/ by means of membrane filtration after nitrosoguanidine mutagenesis. Among them, ts42 requiring uracil for normal growth at 48/sup 0/C was investigated. In the absence of uracil, the mutant cells grew normally at 37/sup 0/C and stopped dividing after temperature shift to 48/sup 0/C resulting in filaments of two to four times length of normal rods. The total cell number after the temperature shift increased two to three fold in 90 min and remained constant thereafter. The viable count after the temperature shift to 48/sup 0/C, increased 1.5 to 2 fold in initial 60 min and then decreased exponentially. A rapid restoration of colony forming ability was shown when the mutant cells were shifted back to the permissive temperature after 120 to 180 min of incubation at 48/sup 0/C or when uracil was introduced to the culture at 48/sup 0/C. This recovery of viability was partly observed even in the presence of chloramphenicol. The synthesis of RNA of this mutant was shown to decline 20 min after the temperature shift to 48/sup 0/C whereas the syntheses of DNA and protein proceeded for more than 80 min at that  More>>
Publication Date:
Jan 01, 1976
Product Type:
Journal Article
Reference Number:
EDB-77-101930
Resource Relation:
Journal Name: Agric. Biol. Chem. (Tokyo); (Japan); Journal Volume: 40:11
Subject:
59 BASIC BIOLOGICAL SCIENCES; BACILLUS SUBTILIS; MUTANTS; TEMPERATURE EFFECTS; RNA; BIOSYNTHESIS; BIOLOGICAL REGENERATION; CHLORAMPHENICOL; GROWTH; HEAT; NUTRITIONAL DEFICIENCY; SENSITIVITY; TEMPERATURE DEPENDENCE; URACILS; ANTIBIOTICS; AZINES; BACILLUS; BACTERIA; BIOLOGICAL RECOVERY; DRUGS; ENERGY; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PYRIMIDINES; RECOVERY; SYNTHESIS; 550200* - Biochemistry; 550700 - Microbiology
OSTI ID:
7110290
Research Organizations:
Tokyo Univ.
Country of Origin:
Japan
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: ABCHA
Submitting Site:
TIC
Size:
Pages: 2237-2243
Announcement Date:
Aug 01, 1977

Journal Article:

Citation Formats

Nagai, K, Some, H, and Tamura, G. Thermosensitive mutant of Bacillus subtilis deficient in uracil and cell division. Japan: N. p., 1976. Web.
Nagai, K, Some, H, & Tamura, G. Thermosensitive mutant of Bacillus subtilis deficient in uracil and cell division. Japan.
Nagai, K, Some, H, and Tamura, G. 1976. "Thermosensitive mutant of Bacillus subtilis deficient in uracil and cell division." Japan.
@misc{etde_7110290,
title = {Thermosensitive mutant of Bacillus subtilis deficient in uracil and cell division}
author = {Nagai, K, Some, H, and Tamura, G}
abstractNote = {Thermonsensitive division mutants were derived from Bacillus subtilis Marburg 168 thy trp/sub 2/ by means of membrane filtration after nitrosoguanidine mutagenesis. Among them, ts42 requiring uracil for normal growth at 48/sup 0/C was investigated. In the absence of uracil, the mutant cells grew normally at 37/sup 0/C and stopped dividing after temperature shift to 48/sup 0/C resulting in filaments of two to four times length of normal rods. The total cell number after the temperature shift increased two to three fold in 90 min and remained constant thereafter. The viable count after the temperature shift to 48/sup 0/C, increased 1.5 to 2 fold in initial 60 min and then decreased exponentially. A rapid restoration of colony forming ability was shown when the mutant cells were shifted back to the permissive temperature after 120 to 180 min of incubation at 48/sup 0/C or when uracil was introduced to the culture at 48/sup 0/C. This recovery of viability was partly observed even in the presence of chloramphenicol. The synthesis of RNA of this mutant was shown to decline 20 min after the temperature shift to 48/sup 0/C whereas the syntheses of DNA and protein proceeded for more than 80 min at that temperature. No newly isolated uracil requiring mutants formed filaments in the medium lacking uracil or showed growth pattern like ts42.}
journal = {Agric. Biol. Chem. (Tokyo); (Japan)}
volume = {40:11}
journal type = {AC}
place = {Japan}
year = {1976}
month = {Jan}
}