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Effect of salt on a thermosensitive mutant of Bacillus subtilis deficient in uracil and cell division

Journal Article:

Abstract

A thermosensitive uracil requiring mutant of Bacillus subtilis Marburg 168 thy trp/sub 2/ ts42 was examined as to the colony forming ability at the permissive and nonpermissive temperatures. The viability of the mutant cells decreased rapidly at the restrictive temperature in the modified Woese's (MW) medium. However, the cells retained viability when sodium succinate or potassium chloride was added to the medium at that temperature although uracil deficiency was unchanged. A little but significant incorporation of adenine-8-/sup 14/C into RNA still continued even after the incorporation of N-acetyl-/sup 3/H-D-glucosamine into acid insoluble fraction of the cells terminated in the MW medium at 48/sup 0/C. Both incorporations as well as increase of absorbance were slowed down in the presence of sodium succinate at 48/sup 0/C. This mutant, ts-42, was more sensitive to deoxycholate (DOC) than the parent strain. The restoration of colony forming ability after the temperature shift back to 37/sup 0/C was suppressed by the addition of DOC to the medium. However, the cell became resistant to DOC when uracil was added to the medium prior to the temperature shift.
Publication Date:
Jan 01, 1976
Product Type:
Journal Article
Reference Number:
EDB-77-101945
Resource Relation:
Journal Name: Agric. Biol. Chem. (Tokyo); (Japan); Journal Volume: 40:11
Subject:
59 BASIC BIOLOGICAL SCIENCES; BACILLUS SUBTILIS; MUTANTS; POTASSIUM CHLORIDES; BIOLOGICAL EFFECTS; SODIUM COMPOUNDS; BIOLOGICAL REGENERATION; BIOSYNTHESIS; CARBON 14 COMPOUNDS; GLUCOSAMINE; NUTRITIONAL DEFICIENCY; RNA; SALTS; SINGLE CELL PROTEIN; TEMPERATURE EFFECTS; TRITIUM COMPOUNDS; URACILS; VIABILITY; ALKALI METAL COMPOUNDS; AMINES; AZINES; BACILLUS; BACTERIA; BIOLOGICAL RECOVERY; CARBOHYDRATES; CHLORIDES; CHLORINE COMPOUNDS; HALIDES; HALOGEN COMPOUNDS; HETEROCYCLIC COMPOUNDS; HEXOSAMINES; HEXOSES; HYDROXY COMPOUNDS; LABELLED COMPOUNDS; MICROORGANISMS; MONOSACCHARIDES; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; POTASSIUM COMPOUNDS; PYRIMIDINES; RECOVERY; SACCHARIDES; SYNTHESIS; 550301* - Cytology- Tracer Techniques; 550201 - Biochemistry- Tracer Techniques; 550701 - Microbiology- Tracer Techniques
OSTI ID:
7110280
Research Organizations:
Tokyo Univ.
Country of Origin:
Japan
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: ABCHA
Submitting Site:
TIC
Size:
Pages: 2245-2249
Announcement Date:

Journal Article:

Citation Formats

Miyazaki, N, Nagai, K, and Tamura, G. Effect of salt on a thermosensitive mutant of Bacillus subtilis deficient in uracil and cell division. Japan: N. p., 1976. Web.
Miyazaki, N, Nagai, K, & Tamura, G. Effect of salt on a thermosensitive mutant of Bacillus subtilis deficient in uracil and cell division. Japan.
Miyazaki, N, Nagai, K, and Tamura, G. 1976. "Effect of salt on a thermosensitive mutant of Bacillus subtilis deficient in uracil and cell division." Japan.
@misc{etde_7110280,
title = {Effect of salt on a thermosensitive mutant of Bacillus subtilis deficient in uracil and cell division}
author = {Miyazaki, N, Nagai, K, and Tamura, G}
abstractNote = {A thermosensitive uracil requiring mutant of Bacillus subtilis Marburg 168 thy trp/sub 2/ ts42 was examined as to the colony forming ability at the permissive and nonpermissive temperatures. The viability of the mutant cells decreased rapidly at the restrictive temperature in the modified Woese's (MW) medium. However, the cells retained viability when sodium succinate or potassium chloride was added to the medium at that temperature although uracil deficiency was unchanged. A little but significant incorporation of adenine-8-/sup 14/C into RNA still continued even after the incorporation of N-acetyl-/sup 3/H-D-glucosamine into acid insoluble fraction of the cells terminated in the MW medium at 48/sup 0/C. Both incorporations as well as increase of absorbance were slowed down in the presence of sodium succinate at 48/sup 0/C. This mutant, ts-42, was more sensitive to deoxycholate (DOC) than the parent strain. The restoration of colony forming ability after the temperature shift back to 37/sup 0/C was suppressed by the addition of DOC to the medium. However, the cell became resistant to DOC when uracil was added to the medium prior to the temperature shift.}
journal = {Agric. Biol. Chem. (Tokyo); (Japan)}
volume = {40:11}
journal type = {AC}
place = {Japan}
year = {1976}
month = {Jan}
}