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Core antigen and circulating anti-core antibody in hepatitis B infection

Journal Article:

Abstract

Core antigen was obtained from the sera of persistent chronic carriers of hepatitis B virus by centrifugation and treatment with Nonidet P40 and 2-mercaptoethanol. The separated core antigen was radiolabelled and identified as a nucleoprotein structure of buoyant density 1.36 g/cm/sup 3/ and possessing an isoelectric point of 4.4. This material was employed in a radioimmnoassay procedure of high sensitivity for the detection of core antibody. In a series of sera from patients with acute type B hepatitis, core antibody was demonstrated 2 to 3 weeks after the onset of jaundice during the period of surface antigenaemia. The presence of core antibody may therefore provide an accurate serological marker for the detection of active or recent virus replication in future epidemiological studies of hepatitis B infection.
Authors:
Howard, C R; Zuckerman, A J [1] 
  1. London School of Hygiene and Tropical Medicine (UK)
Publication Date:
Feb 01, 1977
Product Type:
Journal Article
Reference Number:
AIX-08-310510; EDB-77-102032
Resource Relation:
Journal Name: J. Immunol. Methods; (Netherlands); Journal Volume: 14:3; Other Information: 19 refs., 7 figs
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; ANTIBODIES; RADIOIMMUNOASSAY; ANTIGENS; BLOOD SERUM; DOSE-RESPONSE RELATIONSHIPS; HEPATITIS; LABELLED COMPOUNDS; MAN; PRECIPITATION; QUANTITATIVE CHEMICAL ANALYSIS; SAMPLE PREPARATION; SENSITIVITY; THIOLS; ULTRACENTRIFUGATION; ANIMALS; CENTRIFUGATION; CHEMICAL ANALYSIS; DIGESTIVE SYSTEM DISEASES; DISEASES; ISOTOPE APPLICATIONS; MAMMALS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PRIMATES; RADIOASSAY; SEPARATION PROCESSES; TRACER TECHNIQUES; VERTEBRATES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
OSTI ID:
7110234
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: JIMMB
Submitting Site:
INIS
Size:
Pages: 291-301
Announcement Date:

Journal Article:

Citation Formats

Howard, C R, and Zuckerman, A J. Core antigen and circulating anti-core antibody in hepatitis B infection. Netherlands: N. p., 1977. Web.
Howard, C R, & Zuckerman, A J. Core antigen and circulating anti-core antibody in hepatitis B infection. Netherlands.
Howard, C R, and Zuckerman, A J. 1977. "Core antigen and circulating anti-core antibody in hepatitis B infection." Netherlands.
@misc{etde_7110234,
title = {Core antigen and circulating anti-core antibody in hepatitis B infection}
author = {Howard, C R, and Zuckerman, A J}
abstractNote = {Core antigen was obtained from the sera of persistent chronic carriers of hepatitis B virus by centrifugation and treatment with Nonidet P40 and 2-mercaptoethanol. The separated core antigen was radiolabelled and identified as a nucleoprotein structure of buoyant density 1.36 g/cm/sup 3/ and possessing an isoelectric point of 4.4. This material was employed in a radioimmnoassay procedure of high sensitivity for the detection of core antibody. In a series of sera from patients with acute type B hepatitis, core antibody was demonstrated 2 to 3 weeks after the onset of jaundice during the period of surface antigenaemia. The presence of core antibody may therefore provide an accurate serological marker for the detection of active or recent virus replication in future epidemiological studies of hepatitis B infection.}
journal = {J. Immunol. Methods; (Netherlands)}
volume = {14:3}
journal type = {AC}
place = {Netherlands}
year = {1977}
month = {Feb}
}