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Biogeochemistry of the stable hydrogen isotopes

Abstract

The fractionation of H isotopes between the water in the growth medium and the organically bonded H from microalgae cultured under conditions, where light intensity and wavelength, temperature, nutrient availability, and the H isotope ratio of the water were controlled, is reproducible and light dependent. All studies were based either on the H isotope ratios of the total organic H or on the lipids, where most of the H is firmly bonded to C. H bonded into other macromolecules, proteins, carbohydrates and nucleic acids, does not exchange with water, when algae are incubated in water enriched with deuterium. Only after the destruction of quaternary H bonds are labile hydrogens in macromolecules free to exchange with water. By growing algae (18 strains), including blue-green algae, green algae and diatoms, in continuous light, the isotope fractionations in photosynthesis were reproducibly -93 to -178 per thousand, depending on the organism tested. This fractionation was not temperature dependent. Microalgae grown in total darkness with an organic substrate did not show the isotope fractionation seen in cells grown in light. In both light- and dark-grown algae, however, additional depletion of deuterium (-30 to -60 per thousand) in cellular organic matter occurs during the metabolism of  More>>
Authors:
Estep, M F; Hoering, T C [1] 
  1. Carnegie Institution of Washington, DC (USA)
Publication Date:
Aug 01, 1980
Product Type:
Journal Article
Reference Number:
AIX-11-565081; EDB-81-017812
Resource Relation:
Journal Name: Geochim. Cosmochim. Acta; (United Kingdom); Journal Volume: 44:8
Subject:
54 ENVIRONMENTAL SCIENCES; ALGAE; HYDROGEN ISOTOPES; ISOTOPE SEPARATION; DEUTERIUM; HYDROGEN 1; ISOTOPE RATIO; ISOTOPIC EXCHANGE; ORGANIC COMPOUNDS; PLANT GROWTH; VARIATIONS; VISIBLE RADIATION; WATER; ELECTROMAGNETIC RADIATION; GROWTH; HYDROGEN COMPOUNDS; ISOTOPES; LIGHT NUCLEI; NUCLEI; ODD-EVEN NUCLEI; ODD-ODD NUCLEI; OXYGEN COMPOUNDS; PLANTS; RADIATIONS; SEPARATION PROCESSES; STABLE ISOTOPES; 520302* - Environment, Aquatic- Radioactive Materials Monitoring & Transport- Aquatic Ecosystems & Food Chains- (-1987)
OSTI ID:
7101856
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: GCACA
Submitting Site:
INIS
Size:
Pages: 1197-1206
Announcement Date:

Citation Formats

Estep, M F, and Hoering, T C. Biogeochemistry of the stable hydrogen isotopes. United Kingdom: N. p., 1980. Web. doi:10.1016/0016-7037(80)90073-3.
Estep, M F, & Hoering, T C. Biogeochemistry of the stable hydrogen isotopes. United Kingdom. doi:10.1016/0016-7037(80)90073-3.
Estep, M F, and Hoering, T C. 1980. "Biogeochemistry of the stable hydrogen isotopes." United Kingdom. doi:10.1016/0016-7037(80)90073-3. https://www.osti.gov/servlets/purl/10.1016/0016-7037(80)90073-3.
@misc{etde_7101856,
title = {Biogeochemistry of the stable hydrogen isotopes}
author = {Estep, M F, and Hoering, T C}
abstractNote = {The fractionation of H isotopes between the water in the growth medium and the organically bonded H from microalgae cultured under conditions, where light intensity and wavelength, temperature, nutrient availability, and the H isotope ratio of the water were controlled, is reproducible and light dependent. All studies were based either on the H isotope ratios of the total organic H or on the lipids, where most of the H is firmly bonded to C. H bonded into other macromolecules, proteins, carbohydrates and nucleic acids, does not exchange with water, when algae are incubated in water enriched with deuterium. Only after the destruction of quaternary H bonds are labile hydrogens in macromolecules free to exchange with water. By growing algae (18 strains), including blue-green algae, green algae and diatoms, in continuous light, the isotope fractionations in photosynthesis were reproducibly -93 to -178 per thousand, depending on the organism tested. This fractionation was not temperature dependent. Microalgae grown in total darkness with an organic substrate did not show the isotope fractionation seen in cells grown in light. In both light- and dark-grown algae, however, additional depletion of deuterium (-30 to -60 per thousand) in cellular organic matter occurs during the metabolism of carbohydrates to form lipids. Plants from several natural populations also fractionated isotopes during photosynthesis by an average of -90 to -110 per thousand. In addition, the organically bonded H in nonsaponifiable lipids was further fractionated by -80 per thousand from that in saponifiable lipids, isolated from two geographically distinct populations of marsh plants. This difference between H isotope ratios of these two groups of lipids provides an endogenous isotopic marker.}
doi = {10.1016/0016-7037(80)90073-3}
journal = {Geochim. Cosmochim. Acta; (United Kingdom)}
volume = {44:8}
journal type = {AC}
place = {United Kingdom}
year = {1980}
month = {Aug}
}