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Immunoassay of DNA damage

Journal Article:

Abstract

The direct photomodification of DNA by ultraviolet light or the photo-induced addition of exogenous compounds to DNA components results in alterations of DNA structure ranging from subtle to profound. There are two consequences of these conformational changes. First, cells in which the DNA has been damaged are capable of executing repair steps. Second, the DNA which is usually of very low immunogenicity now becomes highly antigenic. This latter property has allowed the production of a series of monoclonal antibodies that recognize photo-induced DNA damage. Monoclonal antibodies have been generated that recognize the 4',5'-monoadduct and the crosslink of 8-methoxypsoralen in DNA. In addition, another antibody has been prepared which recognizes the furan-side monoadduct of 6,4,4'-trimethylangelicin in DNA. These monoclonal antibodies have been characterized as to sensitivity and specificity using non-competitive and competitive enzyme-linked-immunosorbent assays (ELISA).
Publication Date:
Sep 01, 1988
Product Type:
Journal Article
Reference Number:
GBN-88-004783; EDB-88-187479
Resource Relation:
Journal Name: Photochem. Photobiol.; (United Kingdom); Journal Volume: 48:3
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DNA ADDUCTS; ENZYME IMMUNOASSAY; ULTRAVIOLET RADIATION; MONOCLONAL ANTIBODIES; PHOTOCHEMISTRY; PSORALEN; SENSITIVITY; SPECIFICITY; ADDUCTS; ANTIBODIES; ANTICOAGULANTS; CHEMISTRY; COUMARINS; DRUGS; ELECTROMAGNETIC RADIATION; HEMATOLOGIC AGENTS; HETEROCYCLIC COMPOUNDS; IMMUNOASSAY; ORGANIC COMPOUNDS; ORGANIC OXYGEN COMPOUNDS; RADIATIONS; 560120* - Radiation Effects on Biochemicals, Cells, & Tissue Culture
OSTI ID:
7092362
Research Organizations:
Yale Univ., New Haven, CT (USA); Columbia Univ., New York (US). School of Public Health
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: PHCBA
Submitting Site:
GBN
Size:
Pages: 321-328
Announcement Date:
Nov 01, 1988

Journal Article:

Citation Formats

Gasparro, F P, and Santella, R M. Immunoassay of DNA damage. United Kingdom: N. p., 1988. Web.
Gasparro, F P, & Santella, R M. Immunoassay of DNA damage. United Kingdom.
Gasparro, F P, and Santella, R M. 1988. "Immunoassay of DNA damage." United Kingdom.
@misc{etde_7092362,
title = {Immunoassay of DNA damage}
author = {Gasparro, F P, and Santella, R M}
abstractNote = {The direct photomodification of DNA by ultraviolet light or the photo-induced addition of exogenous compounds to DNA components results in alterations of DNA structure ranging from subtle to profound. There are two consequences of these conformational changes. First, cells in which the DNA has been damaged are capable of executing repair steps. Second, the DNA which is usually of very low immunogenicity now becomes highly antigenic. This latter property has allowed the production of a series of monoclonal antibodies that recognize photo-induced DNA damage. Monoclonal antibodies have been generated that recognize the 4',5'-monoadduct and the crosslink of 8-methoxypsoralen in DNA. In addition, another antibody has been prepared which recognizes the furan-side monoadduct of 6,4,4'-trimethylangelicin in DNA. These monoclonal antibodies have been characterized as to sensitivity and specificity using non-competitive and competitive enzyme-linked-immunosorbent assays (ELISA).}
journal = {Photochem. Photobiol.; (United Kingdom)}
volume = {48:3}
journal type = {AC}
place = {United Kingdom}
year = {1988}
month = {Sep}
}