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Ultraviolet radiation-induced mutability of isogenic uvrA and uvrB strains of Escherichia coli K-12 W3110

Journal Article:

Abstract

Escherichia coli K-12 W3110 uvrB5 strain has been shown to have a higher uv-induced reversion frequency than its wild-type parent when plotted on the basis of mutation frequency versus survival. However for the E. coli B/r WP2s uvrA strain this higher mutability has been observed only at survival levels of 80 to 100%. A study was undertaken to determine if ly to the uvrA and uvrB mutations, or to other genetic background differences. Isogenic strains of E. coli K-12 W3110 carrying uvrA6, uvrB5, uvrA6, and uvrB5, and the uvrA allele from E.coli B/r WP2s were used. Results indicate that the enrichment of minimal medium with a small amount of nutrient broth is sufficient to inhibit minimal medium recovery (MMR) and to enhance leu/sup +/ reversion of the leu B missense mutation in these uvr/sup -/ strains. This suggests that there may be a relationship between MMR and error-free postreplication repair. Further research is in progress to clarify the relationship between MMR and broth enhancement of uv-induced mutagenesis in uvr/sup -/ strains of E. Coli K-12 W3110.
Authors:
Barfknecht, T R; Smith, K C [1] 
  1. Stanford Univ., Calif. (USA). Dept. of Radiology
Publication Date:
Dec 01, 1977
Product Type:
Journal Article
Reference Number:
AIX-09-365362; EDB-78-091946
Resource Relation:
Journal Name: Photochem. Photobiol.; (United Kingdom); Journal Volume: 26:6
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CULTURE MEDIA; RADIOSENSITIVITY EFFECTS; ESCHERICHIA COLI; GENETIC RADIATION EFFECTS; MUTATION FREQUENCY; GENETIC VARIABILITY; ULTRAVIOLET RADIATION; MUTAGENESIS; BIOLOGICAL RECOVERY; GENOTYPE; NUTRIENTS; SURVIVAL CURVES; BACTERIA; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL VARIABILITY; ELECTROMAGNETIC RADIATION; GENETIC EFFECTS; MICROORGANISMS; RADIATION EFFECTS; RADIATIONS; RECOVERY; 560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)
OSTI ID:
7090678
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: PHCBA
Submitting Site:
INIS
Size:
Pages: 643-645
Announcement Date:
Mar 01, 1978

Journal Article:

Citation Formats

Barfknecht, T R, and Smith, K C. Ultraviolet radiation-induced mutability of isogenic uvrA and uvrB strains of Escherichia coli K-12 W3110. United Kingdom: N. p., 1977. Web.
Barfknecht, T R, & Smith, K C. Ultraviolet radiation-induced mutability of isogenic uvrA and uvrB strains of Escherichia coli K-12 W3110. United Kingdom.
Barfknecht, T R, and Smith, K C. 1977. "Ultraviolet radiation-induced mutability of isogenic uvrA and uvrB strains of Escherichia coli K-12 W3110." United Kingdom.
@misc{etde_7090678,
title = {Ultraviolet radiation-induced mutability of isogenic uvrA and uvrB strains of Escherichia coli K-12 W3110}
author = {Barfknecht, T R, and Smith, K C}
abstractNote = {Escherichia coli K-12 W3110 uvrB5 strain has been shown to have a higher uv-induced reversion frequency than its wild-type parent when plotted on the basis of mutation frequency versus survival. However for the E. coli B/r WP2s uvrA strain this higher mutability has been observed only at survival levels of 80 to 100%. A study was undertaken to determine if ly to the uvrA and uvrB mutations, or to other genetic background differences. Isogenic strains of E. coli K-12 W3110 carrying uvrA6, uvrB5, uvrA6, and uvrB5, and the uvrA allele from E.coli B/r WP2s were used. Results indicate that the enrichment of minimal medium with a small amount of nutrient broth is sufficient to inhibit minimal medium recovery (MMR) and to enhance leu/sup +/ reversion of the leu B missense mutation in these uvr/sup -/ strains. This suggests that there may be a relationship between MMR and error-free postreplication repair. Further research is in progress to clarify the relationship between MMR and broth enhancement of uv-induced mutagenesis in uvr/sup -/ strains of E. Coli K-12 W3110.}
journal = {Photochem. Photobiol.; (United Kingdom)}
volume = {26:6}
journal type = {AC}
place = {United Kingdom}
year = {1977}
month = {Dec}
}