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Production of carboxy-terminal specific antiserum against glucagon

Journal Article:

Abstract

To produce carboxy-terminal specific antisera against glucagon was coupled mainly via its amino terminal histidine to thyroglobulin, using the amino group reactive pentandiol at pH 7.0 for the conjugation procedure. After repeated immunization of guinea pigs and rabbits, the antisera were obtained. The titer of guinea pig antiserum against glucagon was 1:3000-1:35000 and affinity constant was 9.3 x 10[sup 10]-11.4 x 10[sup 10] l [center dot] mol[sup -1]. There were no cross reaction with GIP, INS, Copeptide and gastrin. The titer of rabbit antiserum against glucagon was 1:900-1:9000 and affinity constant was 0.36 x 10[sup 10]-3.9 x 10[sup 10] l [center dot] mol[sup -1]. There were no cross reaction with INS, C-peptide and gastrin. The cross reaction with GIP was 0.02%.
Authors:
Yibing, Liu; Shiquan, Han [1] 
  1. Academia Sinica, Beijing, BJ (China). Inst. of Atomic Energy
Publication Date:
Feb 01, 1993
Product Type:
Journal Article
Reference Number:
AIX-25-049586; EDB-94-112409
Resource Relation:
Journal Name: Tongweisu (Journal of Isotopes); (China); Journal Volume: 6:1
Subject:
60 APPLIED LIFE SCIENCES; GLUCAGON; IMMUNE SERUMS; ANTIGENS; IMMUNOGLOBULINS; IODINE 125; RADIOIMMUNOASSAY; BETA DECAY RADIOISOTOPES; BIOASSAY; DAYS LIVING RADIOISOTOPES; DIAGNOSTIC TECHNIQUES; ELECTRON CAPTURE RADIOISOTOPES; GLOBULINS; HORMONES; IMMUNOASSAY; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PEPTIDE HORMONES; PEPTIDES; POLYPEPTIDES; PROTEINS; RADIOASSAY; RADIOIMMUNODETECTION; RADIOISOTOPES; TRACER TECHNIQUES; 553006* - Agriculture & Food Technology- Other Agricultural Applications- (1987-)
OSTI ID:
7081787
Country of Origin:
China
Language:
Chinese
Other Identifying Numbers:
Journal ID: ISSN 1000-7512; CODEN: TONGEM
Submitting Site:
INIS
Size:
Pages: 18-20
Announcement Date:

Journal Article:

Citation Formats

Yibing, Liu, and Shiquan, Han. Production of carboxy-terminal specific antiserum against glucagon. China: N. p., 1993. Web.
Yibing, Liu, & Shiquan, Han. Production of carboxy-terminal specific antiserum against glucagon. China.
Yibing, Liu, and Shiquan, Han. 1993. "Production of carboxy-terminal specific antiserum against glucagon." China.
@misc{etde_7081787,
title = {Production of carboxy-terminal specific antiserum against glucagon}
author = {Yibing, Liu, and Shiquan, Han}
abstractNote = {To produce carboxy-terminal specific antisera against glucagon was coupled mainly via its amino terminal histidine to thyroglobulin, using the amino group reactive pentandiol at pH 7.0 for the conjugation procedure. After repeated immunization of guinea pigs and rabbits, the antisera were obtained. The titer of guinea pig antiserum against glucagon was 1:3000-1:35000 and affinity constant was 9.3 x 10[sup 10]-11.4 x 10[sup 10] l [center dot] mol[sup -1]. There were no cross reaction with GIP, INS, Copeptide and gastrin. The titer of rabbit antiserum against glucagon was 1:900-1:9000 and affinity constant was 0.36 x 10[sup 10]-3.9 x 10[sup 10] l [center dot] mol[sup -1]. There were no cross reaction with INS, C-peptide and gastrin. The cross reaction with GIP was 0.02%.}
journal = {Tongweisu (Journal of Isotopes); (China)}
volume = {6:1}
journal type = {AC}
place = {China}
year = {1993}
month = {Feb}
}