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Gel-chromatographic and light scattering study of the salmonella typhi endotoxin

Journal Article:

Abstract

The endotoxin of Salmonella typhi, strain 0-901 extracted with 1 M sodium chloride was studied by gel-chromatography and light scattering. The extracted material consisted of two components: a high molecular weight endotoxin (5.6 milion dalton) and a lower molecular weight protein-polysaccharide complex (less than 66,000 dalton). The endotoxin component proved to be a highly polydispersed material. Estimation of various averages of gyration radii suggested a more compact structure of endotoxin particles than those obtained by the Boivin extraction method, possibly due to the tertiary structuring of polypeptide chains in the protein-lipopolysaccharide complex of the endotoxin particle.
Authors:
Dezhelici, G; Dezhelici, N; Jusici, D [1] 
  1. Zagreb Univ. (Yugoslavia)
Publication Date:
Jan 01, 1977
Product Type:
Journal Article
Reference Number:
AIX-08-339874; EDB-78-018142
Resource Relation:
Journal Name: C. R. Hebd. Seances Acad. Agric. Fr.; (Yugoslavia); Journal Volume: 49:1
Subject:
59 BASIC BIOLOGICAL SCIENCES; ENDOTOXINS; CHROMATOGRAPHY; GELS; SALMONELLA; SCATTERING; SODIUM CHLORIDES; VISIBLE RADIATION; ALKALI METAL COMPOUNDS; ANTIGENS; BACTERIA; CHLORIDES; CHLORINE COMPOUNDS; COLLOIDS; DISPERSIONS; ELECTROMAGNETIC RADIATION; HALIDES; HALOGEN COMPOUNDS; MICROORGANISMS; RADIATIONS; SEPARATION PROCESSES; SODIUM COMPOUNDS; TOXIC MATERIALS; TOXINS; 550700* - Microbiology
OSTI ID:
7077463
Country of Origin:
Yugoslavia
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: CRHAA
Submitting Site:
INIS
Size:
Pages: 149-162
Announcement Date:

Journal Article:

Citation Formats

Dezhelici, G, Dezhelici, N, and Jusici, D. Gel-chromatographic and light scattering study of the salmonella typhi endotoxin. Yugoslavia: N. p., 1977. Web.
Dezhelici, G, Dezhelici, N, & Jusici, D. Gel-chromatographic and light scattering study of the salmonella typhi endotoxin. Yugoslavia.
Dezhelici, G, Dezhelici, N, and Jusici, D. 1977. "Gel-chromatographic and light scattering study of the salmonella typhi endotoxin." Yugoslavia.
@misc{etde_7077463,
title = {Gel-chromatographic and light scattering study of the salmonella typhi endotoxin}
author = {Dezhelici, G, Dezhelici, N, and Jusici, D}
abstractNote = {The endotoxin of Salmonella typhi, strain 0-901 extracted with 1 M sodium chloride was studied by gel-chromatography and light scattering. The extracted material consisted of two components: a high molecular weight endotoxin (5.6 milion dalton) and a lower molecular weight protein-polysaccharide complex (less than 66,000 dalton). The endotoxin component proved to be a highly polydispersed material. Estimation of various averages of gyration radii suggested a more compact structure of endotoxin particles than those obtained by the Boivin extraction method, possibly due to the tertiary structuring of polypeptide chains in the protein-lipopolysaccharide complex of the endotoxin particle.}
journal = {C. R. Hebd. Seances Acad. Agric. Fr.; (Yugoslavia)}
volume = {49:1}
journal type = {AC}
place = {Yugoslavia}
year = {1977}
month = {Jan}
}