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Enzymatic conversion of starch in twin-screw HTST-extruder

Abstract

Wheat starch was liquefied to DE 25-30 employing heat stable ..cap alpha..-amylase and twin-screw Werner and Pfleiderer Continua 58 HTST-extruder. Most significant reduction in batch saccharification time was obtained when starch was liquefied as a slurry containing 60% water at 120/sup 0/C mass temperature, feed rate 1 500 g min/sup -1/, screw rotation rate 250 min/sup -1/, and 0.9% (w/w, d.s.) Novo Termamyl 120 L ..cap alpha..-amylase was added immediately after initiation of gelatinization in the extruder. Saccharification was carried out at 60/sup 0/C employing 0.36 (w/w, d.s.) Novo glucoamylase 150 L to reach a DE 96 in 22 h. Best total conversion was, however, obtained when also saccharification was initiated in the extruder by adding glucoamylase just before the die element, after lowering mass temperature to 60/sup 0/C, and by allowing the saccharification to continue at 60/sup 0/C after extrusion processing to reach a DE 94 in 5 h and DE 97 in 21 h.
Publication Date:
Dec 01, 1983
Product Type:
Journal Article
Reference Number:
EDB-84-106536
Resource Relation:
Journal Name: Starch/Staerke; (Germany, Federal Republic of); Journal Volume: 35:12
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; STARCH; ENZYMATIC HYDROLYSIS; AMYLASE; EXTRUSION; GELS; GLUCOSE; LIQUEFACTION; SACCHARIFICATION; SLURRIES; ALDEHYDES; CARBOHYDRATES; CHEMICAL REACTIONS; COLLOIDS; DECOMPOSITION; DISPERSIONS; ENZYMES; FABRICATION; GLYCOSYL HYDROLASES; HEXOSES; HYDROLASES; HYDROLYSIS; LYSIS; MATERIALS WORKING; MIXTURES; MONOSACCHARIDES; O-GLYCOSYL HYDROLASES; ORGANIC COMPOUNDS; POLYSACCHARIDES; REAGENTS; SACCHARIDES; SOLVOLYSIS; SUSPENSIONS; THERMOCHEMICAL PROCESSES; 140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989); 550200 - Biochemistry
OSTI ID:
7076586
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: STARD
Submitting Site:
HEDB
Size:
Pages: 411-414
Announcement Date:
Apr 01, 1984

Citation Formats

Hakulin, S, Linko, Y Y, Linko, P, Seiler, K, and Seibel, W. Enzymatic conversion of starch in twin-screw HTST-extruder. Germany: N. p., 1983. Web.
Hakulin, S, Linko, Y Y, Linko, P, Seiler, K, & Seibel, W. Enzymatic conversion of starch in twin-screw HTST-extruder. Germany.
Hakulin, S, Linko, Y Y, Linko, P, Seiler, K, and Seibel, W. 1983. "Enzymatic conversion of starch in twin-screw HTST-extruder." Germany.
@misc{etde_7076586,
title = {Enzymatic conversion of starch in twin-screw HTST-extruder}
author = {Hakulin, S, Linko, Y Y, Linko, P, Seiler, K, and Seibel, W}
abstractNote = {Wheat starch was liquefied to DE 25-30 employing heat stable ..cap alpha..-amylase and twin-screw Werner and Pfleiderer Continua 58 HTST-extruder. Most significant reduction in batch saccharification time was obtained when starch was liquefied as a slurry containing 60% water at 120/sup 0/C mass temperature, feed rate 1 500 g min/sup -1/, screw rotation rate 250 min/sup -1/, and 0.9% (w/w, d.s.) Novo Termamyl 120 L ..cap alpha..-amylase was added immediately after initiation of gelatinization in the extruder. Saccharification was carried out at 60/sup 0/C employing 0.36 (w/w, d.s.) Novo glucoamylase 150 L to reach a DE 96 in 22 h. Best total conversion was, however, obtained when also saccharification was initiated in the extruder by adding glucoamylase just before the die element, after lowering mass temperature to 60/sup 0/C, and by allowing the saccharification to continue at 60/sup 0/C after extrusion processing to reach a DE 94 in 5 h and DE 97 in 21 h.}
journal = []
volume = {35:12}
journal type = {AC}
place = {Germany}
year = {1983}
month = {Dec}
}