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/sup 31/P-NMR studies of respiratory regulation in the intact myocardium

Abstract

The mechanism by which mitochondrial respiration is coupled to ATP consumption in intact tissues is unclear. The authors determined the relationship between high-energy phosphate levels and oxygen consumption rate in rat hearts operating over a range of workloads and perfused with different substrates. With pyruvate + glucose perfusion, ADP levels were in general very low, and varied with MVO/sub 2/ yielding an apparent K/sub m/ of 25 +- 5 ..mu..M, suggesting regulation of oxidative phosphorylation through availability of ADP. In contrast, with glucose perfusion in the presence or absence of insulin, ADP levels, ADP/ATP ratio or the phosphate potential were relatively constant over the workload range examined and generally not correlated with alterations in MVO/sub 2/; it is suggested that under these conditions, carbon substrate delivery to the mitochondria may control mitochondrial respiration. The common feature of both of the suggested regulatory mechanisms is substrate limitation which, however, is exercised at different metabolic points depending on the carbon substrate available to the myocardium. 19 refs.; 2 figs.; 1 table.
Publication Date:
Oct 06, 1986
Product Type:
Journal Article
Reference Number:
AIX-18-031643; EDB-87-066575
Resource Relation:
Journal Name: FEBS Lett.; (Netherlands); Journal Volume: 206:2
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; ATP; BIOCHEMICAL REACTION KINETICS; METABOLISM; MITOCHONDRIA; RESPIRATION; ADP; MYOCARDIUM; NMR SPECTRA; NUCLEAR MAGNETIC RESONANCE; PHOSPHORUS 31; PHOSPHORYLATION; RATS; SUBSTRATES; ANIMALS; BODY; CARDIOVASCULAR SYSTEM; CELL CONSTITUENTS; CHEMICAL REACTIONS; HEART; ISOTOPES; KINETICS; LIGHT NUCLEI; MAGNETIC RESONANCE; MAMMALS; MUSCLES; NUCLEI; NUCLEOTIDES; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANOIDS; ORGANS; PHOSPHORUS ISOTOPES; REACTION KINETICS; RESONANCE; RODENTS; SPECTRA; STABLE ISOTOPES; VERTEBRATES; 550200* - Biochemistry; 400102 - Chemical & Spectral Procedures
OSTI ID:
7065827
Research Organizations:
Cardiovascular Div., Dept. of Medicine, Minneapolis VA Medical Center and Univ. of Minnesota, Minneapolis, MN, USA; Minnesota Univ., Navarre, USA. Dept. of Biochemistry; Minnesota Univ., Navarre, USA. Gray Freshwater Biological Inst.
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: FEBLA
Submitting Site:
INIS
Size:
Pages: 257-261
Announcement Date:

Citation Formats

From, A H.L., Petein, M A, Zimmer, S W, Michurski, S P, and Ugurbil, K. /sup 31/P-NMR studies of respiratory regulation in the intact myocardium. Netherlands: N. p., 1986. Web. doi:10.1016/0014-5793(86)80992-9.
From, A H.L., Petein, M A, Zimmer, S W, Michurski, S P, & Ugurbil, K. /sup 31/P-NMR studies of respiratory regulation in the intact myocardium. Netherlands. doi:10.1016/0014-5793(86)80992-9.
From, A H.L., Petein, M A, Zimmer, S W, Michurski, S P, and Ugurbil, K. 1986. "/sup 31/P-NMR studies of respiratory regulation in the intact myocardium." Netherlands. doi:10.1016/0014-5793(86)80992-9. https://www.osti.gov/servlets/purl/10.1016/0014-5793(86)80992-9.
@misc{etde_7065827,
title = {/sup 31/P-NMR studies of respiratory regulation in the intact myocardium}
author = {From, A H.L., Petein, M A, Zimmer, S W, Michurski, S P, and Ugurbil, K}
abstractNote = {The mechanism by which mitochondrial respiration is coupled to ATP consumption in intact tissues is unclear. The authors determined the relationship between high-energy phosphate levels and oxygen consumption rate in rat hearts operating over a range of workloads and perfused with different substrates. With pyruvate + glucose perfusion, ADP levels were in general very low, and varied with MVO/sub 2/ yielding an apparent K/sub m/ of 25 +- 5 ..mu..M, suggesting regulation of oxidative phosphorylation through availability of ADP. In contrast, with glucose perfusion in the presence or absence of insulin, ADP levels, ADP/ATP ratio or the phosphate potential were relatively constant over the workload range examined and generally not correlated with alterations in MVO/sub 2/; it is suggested that under these conditions, carbon substrate delivery to the mitochondria may control mitochondrial respiration. The common feature of both of the suggested regulatory mechanisms is substrate limitation which, however, is exercised at different metabolic points depending on the carbon substrate available to the myocardium. 19 refs.; 2 figs.; 1 table.}
doi = {10.1016/0014-5793(86)80992-9}
journal = {FEBS Lett.; (Netherlands)}
volume = {206:2}
journal type = {AC}
place = {Netherlands}
year = {1986}
month = {Oct}
}