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Lipidperoxidative and proteolytic processes in fungi under the influence of xenobiotica. [Botrytis cinerea; Phytophora infestans]

Abstract

As a model system for the investigation of peroxidative effects in the phytopathogenic fungi Botrytis cinerea in submers culture was used. The influence was investigated of hydrogenperoxide with biochemical and cytological methods. The hyphae showed significant changes of the ultrastructure: swelling and vacuolization of mitochondria, loss of ribosomes, vesiculation of endoplasmic reticulum, degradation and lysis of the cell ribosomes, and deposition of lipid droplets on the membranes and in the cytoplasm, dependent on the concentration of the peroxide. Same effects with higher injuries of the membrane system are found in Phytophthorea infestans. A parallel investigation on fibroblasts showed similar effects: pathological changes of mitochondria, but also pycnosis in the nucleus. Comparative ultrastructure investigations were performed in cultures of Phytophthora infestans with tetrachlorcarbon and chloroneb, a specific fungicide substance with a new mode of action. Scavengers like ..cap alpha..-tocopherol and piperonyl butoxide inhibit the toxic effect of the investigated substances in various degrees. Results are discussed in regard to the effect of a lipid peroxidative and proteolytic attack in pesticides of unknown mechanism of action.
Publication Date:
Jan 01, 1986
Product Type:
Conference
Report Number:
CONF-8607367-
Reference Number:
EDB-88-125685
Resource Relation:
Journal Name: Acta Biol. Hung.; (Hungary); Journal Volume: 37; Conference: 2. European congress on cell biology, Budapest, Hungary, 6 Jul 1986
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; FUNGI; ULTRASTRUCTURAL CHANGES; HYDROGEN PEROXIDE; TOXICITY; BIOLOGICAL ACCUMULATION; CARBON TETRACHLORIDE; CELL MEMBRANES; CYTOPLASM; ENDOPLASMIC RETICULUM; FIBROBLASTS; FUNGICIDES; LIPIDS; MITOCHONDRIA; OXIDATION; PROTEOLYSIS; RESPONSE MODIFYING FACTORS; RIBOSOMES; VITAMIN E; ANIMAL CELLS; CELL CONSTITUENTS; CHEMICAL REACTIONS; CHLORINATED ALIPHATIC HYDROCARBONS; CONNECTIVE TISSUE CELLS; DECOMPOSITION; HALOGENATED ALIPHATIC HYDROCARBONS; HYDROGEN COMPOUNDS; MEMBRANES; MORPHOLOGICAL CHANGES; ORGANIC CHLORINE COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; ORGANOIDS; OXYGEN COMPOUNDS; PEROXIDES; PESTICIDES; PLANTS; SOMATIC CELLS; VITAMINS; 560300* - Chemicals Metabolism & Toxicology
OSTI ID:
7059774
Research Organizations:
Institute of Plant Protection Research, Stahnsdorfer (East Germany)
Country of Origin:
Hungary
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: ABHUE
Submitting Site:
JMT
Size:
Pages: 153
Announcement Date:

Citation Formats

Mueller, H M, Edlich, W, and Lyr, H. Lipidperoxidative and proteolytic processes in fungi under the influence of xenobiotica. [Botrytis cinerea; Phytophora infestans]. Hungary: N. p., 1986. Web.
Mueller, H M, Edlich, W, & Lyr, H. Lipidperoxidative and proteolytic processes in fungi under the influence of xenobiotica. [Botrytis cinerea; Phytophora infestans]. Hungary.
Mueller, H M, Edlich, W, and Lyr, H. 1986. "Lipidperoxidative and proteolytic processes in fungi under the influence of xenobiotica. [Botrytis cinerea; Phytophora infestans]." Hungary.
@misc{etde_7059774,
title = {Lipidperoxidative and proteolytic processes in fungi under the influence of xenobiotica. [Botrytis cinerea; Phytophora infestans]}
author = {Mueller, H M, Edlich, W, and Lyr, H}
abstractNote = {As a model system for the investigation of peroxidative effects in the phytopathogenic fungi Botrytis cinerea in submers culture was used. The influence was investigated of hydrogenperoxide with biochemical and cytological methods. The hyphae showed significant changes of the ultrastructure: swelling and vacuolization of mitochondria, loss of ribosomes, vesiculation of endoplasmic reticulum, degradation and lysis of the cell ribosomes, and deposition of lipid droplets on the membranes and in the cytoplasm, dependent on the concentration of the peroxide. Same effects with higher injuries of the membrane system are found in Phytophthorea infestans. A parallel investigation on fibroblasts showed similar effects: pathological changes of mitochondria, but also pycnosis in the nucleus. Comparative ultrastructure investigations were performed in cultures of Phytophthora infestans with tetrachlorcarbon and chloroneb, a specific fungicide substance with a new mode of action. Scavengers like ..cap alpha..-tocopherol and piperonyl butoxide inhibit the toxic effect of the investigated substances in various degrees. Results are discussed in regard to the effect of a lipid peroxidative and proteolytic attack in pesticides of unknown mechanism of action.}
journal = {Acta Biol. Hung.; (Hungary)}
volume = {37}
place = {Hungary}
year = {1986}
month = {Jan}
}