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Production of amylolytic enzymes by bacillus spp

Abstract

Sixty six bacteria and twenty fungi were isolated from various sources. These varied from rotten fruites to local drinks and soil samples from different parts of Sudan. On the basis of index of amylolytic activity, forty one bacteria and twelve fungi were found to hydrolyse strach. The best ten strach hydrolysing isolates were identified all as bacilli (Bacillus licheniformis SUD-K{sub 1}, SUD-K{sub 2}, SUD-K{sub 4}, SUD-O, SUD-SRW, SUD-BRW, SUD-By, Bacillus subtilis SUD-K{sub 3}, and Bacillus circulans SUD-D and SUD-K{sub 7}). Their amylase productivity was studied with respect to temperature and time. Amylolytic activity was measured by spectrophotometer, the highest activity was produced in around 24 hours of growth in all; six of which gave the highest amylase activity at 50 deg C and the rest at 45C. Based on the thermal production six isolates were chosen for further investigation. These were Bacillus licheniformis SUD-K{sub 1}, SUD-K{sub 2}, SUD-K{sub 4}, SUD-O, Bacillus subtilis SUD-K{sub 3} and Bacillus circulans SUD-K{sub 7}. The inclusion of strach and Mg{sup ++} ions in the culture medium gave the highest enzyme yield. The Ph 9.0 was found to be the optimum for amylase production for all isolates except Bacillus subtilis SUD-K{sub 3} which had an optimum  More>>
Authors:
Dawood, Elham Shareif [1] 
  1. Department of Botany, Faculty of Science, University of Khartoum, Khartoum (Sudan)
Publication Date:
Dec 01, 1997
Product Type:
Miscellaneous
Report Number:
INIS-SD-079
Reference Number:
SCA: 553006; 400101; PA: AIX-30:050960; SN: 99002165061
Resource Relation:
Other Information: DN: 185 refs. , 5 tabs. , 57 figs.; TH: Thesis (Ph.D); PBD: Dec 1997
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; 40 CHEMISTRY; BACILLUS; BACTERIA; ENZYMES; FRUITS; FUNGI; NUMERICAL DATA; SOILS; SPECTROPHOTOMETERS
OSTI ID:
703871
Research Organizations:
University of Khartoum, Khartoum(Sudan)
Country of Origin:
Sudan
Language:
English
Other Identifying Numbers:
Other: ON: DE99638995; TRN: SD9900043050960
Availability:
INIS; OSTI as DE99638995
Submitting Site:
INIS
Size:
93 p.
Announcement Date:

Citation Formats

Dawood, Elham Shareif. Production of amylolytic enzymes by bacillus spp. Sudan: N. p., 1997. Web.
Dawood, Elham Shareif. Production of amylolytic enzymes by bacillus spp. Sudan.
Dawood, Elham Shareif. 1997. "Production of amylolytic enzymes by bacillus spp." Sudan.
@misc{etde_703871,
title = {Production of amylolytic enzymes by bacillus spp}
author = {Dawood, Elham Shareif}
abstractNote = {Sixty six bacteria and twenty fungi were isolated from various sources. These varied from rotten fruites to local drinks and soil samples from different parts of Sudan. On the basis of index of amylolytic activity, forty one bacteria and twelve fungi were found to hydrolyse strach. The best ten strach hydrolysing isolates were identified all as bacilli (Bacillus licheniformis SUD-K{sub 1}, SUD-K{sub 2}, SUD-K{sub 4}, SUD-O, SUD-SRW, SUD-BRW, SUD-By, Bacillus subtilis SUD-K{sub 3}, and Bacillus circulans SUD-D and SUD-K{sub 7}). Their amylase productivity was studied with respect to temperature and time. Amylolytic activity was measured by spectrophotometer, the highest activity was produced in around 24 hours of growth in all; six of which gave the highest amylase activity at 50 deg C and the rest at 45C. Based on the thermal production six isolates were chosen for further investigation. These were Bacillus licheniformis SUD-K{sub 1}, SUD-K{sub 2}, SUD-K{sub 4}, SUD-O, Bacillus subtilis SUD-K{sub 3} and Bacillus circulans SUD-K{sub 7}. The inclusion of strach and Mg{sup ++} ions in the culture medium gave the highest enzyme yield. The Ph 9.0 was found to be the optimum for amylase production for all isolates except Bacillus subtilis SUD-K{sub 3} which had an optimum at pH 7.0. Three isolates (Bacillus licheniformis SUD-K{sub 1}, SUD-K{sub 4} and SUD-O recorded highestamylase production in a medium supplemented with peptone while the rest (Bacillus licheniformis SUD-K{sub 2}, Bacillus subtilis SUD-K{sub 3} and Bacillus circulans SUD-K{sub 7}) gave highest amylase productivity in a medium supplemented with malt extract. Four isolates (Bacillus licheniformis SUD-K{sub 1} and Bacillus subtilis SUD-K{sub 3} gave maximum amylase production in a medium containing 0.5% soluble strach while the rest (gave maximum amylase production at 2%. Soluble strach was found to be best substrate among the different carbon sources tested. The maximum temperature for amylase activity ranged from 60-70 deg C and 1% strach concentration was optimum for all isolates. Addition of different metal ions and different concentration of sodium chloride separately to to the reaction mixture suppressed the enzyme activity. Hydrolysis pattern of the substrate soluble strach by these amylases indicated that higher ratio of oligosaccharides and maltose are the main products and this indicated that these enzymes can be identified as {alpha} amylases. The {alpha} amylases were partially purified up to 15-19 fold of their initial activities. The Km values of all isolates were in the range of 1.25-2.0 mg/ml. (Author) 185 refs. , 5 tabs. , 57 figs.}
place = {Sudan}
year = {1997}
month = {Dec}
}