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Development and application of bio-sensor. Production of ammonia sensor

Abstract

The objectives of this study are to make a biosensor on a trial basis which can instantaneously measure the nitrogen in wastewater, and to develop a wastewater treatment system which is capable of on-line measurement and controlling. The system provides easier operational control relating to such a high efficient treatment as the removal of nitrogen content in wastewater, serving as a solution to the eutrophication problem. It can be applied also to the analysis of fertilizer components for agriculture. Ammonia oxidizing bacteria were immobilized with cellulose acetate film, which is mounted on a diaphragm type oxygen electrode to make a sensor, and its responsibility was studied. The gradient is slow in high concentration but sharp in low concentration, and it seems possible to use it for the measurement for less than 20 ppm nitrogen concentration. The dependence of the sensor including electrodes and activity of bacteria on temperature is large, and the measurement should be made at a constant temperature. The responsibility was best at the pH of 9. The sensor could be used repeatedly for about a month. 7 refs., 10 figs., 1 tab.
Authors:
Totsuka, Yoshiyuki; Matsumoto, Yutaka; Sakata, Tadashi; Nakatsugawa, Shuuji; Nishina, Tokuhiro; Shiozawa, Kanji [1] 
  1. Shizuoka Prefectural Industrial Technology Center, Shizuoka, (Japan)
Publication Date:
Aug 01, 1989
Product Type:
Journal Article
Reference Number:
NEDO-89-930152; EDB-90-042165
Resource Relation:
Journal Name: Shizuoka-Ken Kogyogijutsu Center Kenkyu Hokoku (Japan); (Japan); Journal Volume: 34
Subject:
54 ENVIRONMENTAL SCIENCES; 47 OTHER INSTRUMENTATION; AMMONIA; BACTERIA; OXIDATION; IMMOBILIZED CELLS; CELLULASE; ACETIC ACID; FILMS; LIQUID WASTES; MEASURING METHODS; NITROGEN; ON-LINE MEASUREMENT SYSTEMS; SENSITIVITY; WASTE PROCESSING; EUTROPHICATION; FERTILIZERS; HYDROPONIC CULTURE; CARBOXYLIC ACIDS; CHEMICAL REACTIONS; ELEMENTS; ENZYMES; GLYCOSYL HYDROLASES; HYDRIDES; HYDROGEN COMPOUNDS; HYDROLASES; MANAGEMENT; MICROORGANISMS; MONOCARBOXYLIC ACIDS; NITROGEN COMPOUNDS; NITROGEN HYDRIDES; NONMETALS; O-GLYCOSYL HYDROLASES; ON-LINE SYSTEMS; ORGANIC ACIDS; ORGANIC COMPOUNDS; PROCESSING; WASTE MANAGEMENT; WASTES; 540000* - Environment- (1990-); 440800 - Miscellaneous Instrumentation- (1990-)
OSTI ID:
7019873
Country of Origin:
Japan
Language:
Japanese
Other Identifying Numbers:
Journal ID: ISSN 0910-9323; CODEN: SKGHE
Submitting Site:
NEDO
Size:
Pages: 49-52
Announcement Date:
Mar 15, 1990

Citation Formats

Totsuka, Yoshiyuki, Matsumoto, Yutaka, Sakata, Tadashi, Nakatsugawa, Shuuji, Nishina, Tokuhiro, and Shiozawa, Kanji. Development and application of bio-sensor. Production of ammonia sensor. Japan: N. p., 1989. Web.
Totsuka, Yoshiyuki, Matsumoto, Yutaka, Sakata, Tadashi, Nakatsugawa, Shuuji, Nishina, Tokuhiro, & Shiozawa, Kanji. Development and application of bio-sensor. Production of ammonia sensor. Japan.
Totsuka, Yoshiyuki, Matsumoto, Yutaka, Sakata, Tadashi, Nakatsugawa, Shuuji, Nishina, Tokuhiro, and Shiozawa, Kanji. 1989. "Development and application of bio-sensor. Production of ammonia sensor." Japan.
@misc{etde_7019873,
title = {Development and application of bio-sensor. Production of ammonia sensor}
author = {Totsuka, Yoshiyuki, Matsumoto, Yutaka, Sakata, Tadashi, Nakatsugawa, Shuuji, Nishina, Tokuhiro, and Shiozawa, Kanji}
abstractNote = {The objectives of this study are to make a biosensor on a trial basis which can instantaneously measure the nitrogen in wastewater, and to develop a wastewater treatment system which is capable of on-line measurement and controlling. The system provides easier operational control relating to such a high efficient treatment as the removal of nitrogen content in wastewater, serving as a solution to the eutrophication problem. It can be applied also to the analysis of fertilizer components for agriculture. Ammonia oxidizing bacteria were immobilized with cellulose acetate film, which is mounted on a diaphragm type oxygen electrode to make a sensor, and its responsibility was studied. The gradient is slow in high concentration but sharp in low concentration, and it seems possible to use it for the measurement for less than 20 ppm nitrogen concentration. The dependence of the sensor including electrodes and activity of bacteria on temperature is large, and the measurement should be made at a constant temperature. The responsibility was best at the pH of 9. The sensor could be used repeatedly for about a month. 7 refs., 10 figs., 1 tab.}
journal = []
volume = {34}
journal type = {AC}
place = {Japan}
year = {1989}
month = {Aug}
}