Abstract
Recently fluorescence in situ hybridization protocols have been developed which allow the paining of individual chromosomes using DNA-libraries from sorted human chromosomes. This approach has the particular advantage that radiation induced chromosome translocations can be easily detected, if chromosomes of distinctly different colors take part in the translocation event. To enhance the sensitivity of this approach two metaphase chromosome subsets A and B (A: chromosome 1, 2, 4, 8, 16; B: 3, 5, 9, 10, 13) were simultaneously painted in green and red color. Counterstaining of the chromosomes with DAPI resulted in a third subset which exhibited blue fluorescence only. Green-red, green-blue and red-blue translocation chromosomes could be easily detected after irradiation of lymphocyte cultures with {sup 137}Cs-{gamma}-rays. Analyses of painted chromosomes can be combined with conventional GTG-banding analyses. This new biological dosimeter should become useful to monitor both long term effects of single irradiation events and the cumulative effects of multiple or chronic irradiation exposure. In contrast to translocation scoring based on the analysis of banded chromosomes, this new approach has the particular advantage that a rapid, automated scoring of translocations can now be envisaged. (author).
Citation Formats
Popp, S, and Cremer, T.
Development of a biological dosimeter for translocation scoring based on two-color fluorescence in situ hybridization of chromosome subsets.
Japan: N. p.,
1992.
Web.
Popp, S, & Cremer, T.
Development of a biological dosimeter for translocation scoring based on two-color fluorescence in situ hybridization of chromosome subsets.
Japan.
Popp, S, and Cremer, T.
1992.
"Development of a biological dosimeter for translocation scoring based on two-color fluorescence in situ hybridization of chromosome subsets."
Japan.
@misc{etde_7013636,
title = {Development of a biological dosimeter for translocation scoring based on two-color fluorescence in situ hybridization of chromosome subsets}
author = {Popp, S, and Cremer, T}
abstractNote = {Recently fluorescence in situ hybridization protocols have been developed which allow the paining of individual chromosomes using DNA-libraries from sorted human chromosomes. This approach has the particular advantage that radiation induced chromosome translocations can be easily detected, if chromosomes of distinctly different colors take part in the translocation event. To enhance the sensitivity of this approach two metaphase chromosome subsets A and B (A: chromosome 1, 2, 4, 8, 16; B: 3, 5, 9, 10, 13) were simultaneously painted in green and red color. Counterstaining of the chromosomes with DAPI resulted in a third subset which exhibited blue fluorescence only. Green-red, green-blue and red-blue translocation chromosomes could be easily detected after irradiation of lymphocyte cultures with {sup 137}Cs-{gamma}-rays. Analyses of painted chromosomes can be combined with conventional GTG-banding analyses. This new biological dosimeter should become useful to monitor both long term effects of single irradiation events and the cumulative effects of multiple or chronic irradiation exposure. In contrast to translocation scoring based on the analysis of banded chromosomes, this new approach has the particular advantage that a rapid, automated scoring of translocations can now be envisaged. (author).}
journal = {Journal of Radiation Research; (Japan)}
volume = {33:suppl.}
journal type = {AC}
place = {Japan}
year = {1992}
month = {Mar}
}
title = {Development of a biological dosimeter for translocation scoring based on two-color fluorescence in situ hybridization of chromosome subsets}
author = {Popp, S, and Cremer, T}
abstractNote = {Recently fluorescence in situ hybridization protocols have been developed which allow the paining of individual chromosomes using DNA-libraries from sorted human chromosomes. This approach has the particular advantage that radiation induced chromosome translocations can be easily detected, if chromosomes of distinctly different colors take part in the translocation event. To enhance the sensitivity of this approach two metaphase chromosome subsets A and B (A: chromosome 1, 2, 4, 8, 16; B: 3, 5, 9, 10, 13) were simultaneously painted in green and red color. Counterstaining of the chromosomes with DAPI resulted in a third subset which exhibited blue fluorescence only. Green-red, green-blue and red-blue translocation chromosomes could be easily detected after irradiation of lymphocyte cultures with {sup 137}Cs-{gamma}-rays. Analyses of painted chromosomes can be combined with conventional GTG-banding analyses. This new biological dosimeter should become useful to monitor both long term effects of single irradiation events and the cumulative effects of multiple or chronic irradiation exposure. In contrast to translocation scoring based on the analysis of banded chromosomes, this new approach has the particular advantage that a rapid, automated scoring of translocations can now be envisaged. (author).}
journal = {Journal of Radiation Research; (Japan)}
volume = {33:suppl.}
journal type = {AC}
place = {Japan}
year = {1992}
month = {Mar}
}