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Development of a biological dosimeter for translocation scoring based on two-color fluorescence in situ hybridization of chromosome subsets

Journal Article:

Abstract

Recently fluorescence in situ hybridization protocols have been developed which allow the paining of individual chromosomes using DNA-libraries from sorted human chromosomes. This approach has the particular advantage that radiation induced chromosome translocations can be easily detected, if chromosomes of distinctly different colors take part in the translocation event. To enhance the sensitivity of this approach two metaphase chromosome subsets A and B (A: chromosome 1, 2, 4, 8, 16; B: 3, 5, 9, 10, 13) were simultaneously painted in green and red color. Counterstaining of the chromosomes with DAPI resulted in a third subset which exhibited blue fluorescence only. Green-red, green-blue and red-blue translocation chromosomes could be easily detected after irradiation of lymphocyte cultures with {sup 137}Cs-{gamma}-rays. Analyses of painted chromosomes can be combined with conventional GTG-banding analyses. This new biological dosimeter should become useful to monitor both long term effects of single irradiation events and the cumulative effects of multiple or chronic irradiation exposure. In contrast to translocation scoring based on the analysis of banded chromosomes, this new approach has the particular advantage that a rapid, automated scoring of translocations can now be envisaged. (author).
Authors:
Popp, S; Cremer, T [1] 
  1. Heidelberg Univ. (Germany). Inst. of Human Genetics and Anthropology
Publication Date:
Mar 01, 1992
Product Type:
Journal Article
Reference Number:
JPN-92-009312; EDB-92-161791
Resource Relation:
Journal Name: Journal of Radiation Research; (Japan); Journal Volume: 33:suppl.
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; GENETIC RADIATION EFFECTS; CHROMOSOMAL ABERRATIONS; CESIUM 137; DNA; FLUORESCENCE; HYBRIDIZATION; LYMPHOCYTES; STAINS; ALKALI METAL ISOTOPES; ANIMAL CELLS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BIOLOGICAL RADIATION EFFECTS; BLOOD; BLOOD CELLS; BODY FLUIDS; CESIUM ISOTOPES; CONNECTIVE TISSUE CELLS; GENETIC EFFECTS; INTERMEDIATE MASS NUCLEI; ISOTOPES; LEUKOCYTES; LUMINESCENCE; MATERIALS; MUTATIONS; NUCLEI; NUCLEIC ACIDS; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; RADIATION EFFECTS; RADIOISOTOPES; SOMATIC CELLS; YEARS LIVING RADIOISOTOPES; 560120* - Radiation Effects on Biochemicals, Cells, & Tissue Culture
OSTI ID:
7013636
Country of Origin:
Japan
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0449-3060; CODEN: JRARA
Submitting Site:
JPN
Size:
Pages: 61-70
Announcement Date:

Journal Article:

Citation Formats

Popp, S, and Cremer, T. Development of a biological dosimeter for translocation scoring based on two-color fluorescence in situ hybridization of chromosome subsets. Japan: N. p., 1992. Web.
Popp, S, & Cremer, T. Development of a biological dosimeter for translocation scoring based on two-color fluorescence in situ hybridization of chromosome subsets. Japan.
Popp, S, and Cremer, T. 1992. "Development of a biological dosimeter for translocation scoring based on two-color fluorescence in situ hybridization of chromosome subsets." Japan.
@misc{etde_7013636,
title = {Development of a biological dosimeter for translocation scoring based on two-color fluorescence in situ hybridization of chromosome subsets}
author = {Popp, S, and Cremer, T}
abstractNote = {Recently fluorescence in situ hybridization protocols have been developed which allow the paining of individual chromosomes using DNA-libraries from sorted human chromosomes. This approach has the particular advantage that radiation induced chromosome translocations can be easily detected, if chromosomes of distinctly different colors take part in the translocation event. To enhance the sensitivity of this approach two metaphase chromosome subsets A and B (A: chromosome 1, 2, 4, 8, 16; B: 3, 5, 9, 10, 13) were simultaneously painted in green and red color. Counterstaining of the chromosomes with DAPI resulted in a third subset which exhibited blue fluorescence only. Green-red, green-blue and red-blue translocation chromosomes could be easily detected after irradiation of lymphocyte cultures with {sup 137}Cs-{gamma}-rays. Analyses of painted chromosomes can be combined with conventional GTG-banding analyses. This new biological dosimeter should become useful to monitor both long term effects of single irradiation events and the cumulative effects of multiple or chronic irradiation exposure. In contrast to translocation scoring based on the analysis of banded chromosomes, this new approach has the particular advantage that a rapid, automated scoring of translocations can now be envisaged. (author).}
journal = {Journal of Radiation Research; (Japan)}
volume = {33:suppl.}
journal type = {AC}
place = {Japan}
year = {1992}
month = {Mar}
}