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B-cell stimulating factor

Abstract

BSF-1 was derived from malignant cells and purified by use of various techniques, including adsorption, ion exchange chromatography and reverse phase high performance liquid chromatography. By these techniques, the BSF-1 was purified to homogenity. The high purification of the BSF-1 has made possible the sequencing of the amino acid residues at the N-terminal portion of its protein molecules. From the amino acid sequencing information, a radiolabelled oligonucleotide probe corresponding to portion of the amino acid sequence of the BSF-1 molecule was synthesized and then used to probe a cDNA library prepared from polyadenylated mRNA extracted from cell lines known to produce BSF-1. Through this procedure, a cDNA clone containing the BSF-1 gene was isolated, sequenced and mature BSF-1 expressed. The isolated cDNA clone was then radiolabelled and used as a large probe for screening cDNA libraries of other species of animals for homologous BSF-1 clones.
Publication Date:
Oct 05, 1987
Product Type:
Patent
Report Number:
A
Reference Number:
AIX-19-054923; EDB-88-116524
Resource Relation:
Patent Priority Date: Priority date 19 May 1986, United States of America (USA); Other Information: US priority 864,722
Subject:
59 BASIC BIOLOGICAL SCIENCES; LYMPHOKINES; DNA SEQUENCING; DNA-CLONING; FRACTIONATION; SPECIES DIVERSITY; AMINO ACID SEQUENCE; CLONE CELLS; ION EXCHANGE CHROMATOGRAPHY; LABELLING; LIQUID COLUMN CHROMATOGRAPHY; MESSENGER-RNA; OLIGONUCLEOTIDES; TRACER TECHNIQUES; CELL CULTURES; CHROMATOGRAPHY; CLONING; GROWTH FACTORS; ISOTOPE APPLICATIONS; MITOGENS; MOLECULAR STRUCTURE; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PROTEINS; RNA; SEPARATION PROCESSES; STRUCTURAL CHEMICAL ANALYSIS; 550201* - Biochemistry- Tracer Techniques; 550401 - Genetics- Tracer Techniques
OSTI ID:
7000059
Country of Origin:
South Africa
Language:
English
Availability:
Patents Office, Private Bag X400, Pretoria, 0001, South Africa.
Submitting Site:
INIS
Size:
Pages: 49
Announcement Date:

Citation Formats

Clevenger, W R, Conlon, P J, Eisenman, J R, Gillis, S, Grabstein, K H, Hopp, T P, March, C J, Mochizuki, D Y, Price, V L, and Shanebeck, K D. B-cell stimulating factor. South Africa: N. p., 1987. Web.
Clevenger, W R, Conlon, P J, Eisenman, J R, Gillis, S, Grabstein, K H, Hopp, T P, March, C J, Mochizuki, D Y, Price, V L, & Shanebeck, K D. B-cell stimulating factor. South Africa.
Clevenger, W R, Conlon, P J, Eisenman, J R, Gillis, S, Grabstein, K H, Hopp, T P, March, C J, Mochizuki, D Y, Price, V L, and Shanebeck, K D. 1987. "B-cell stimulating factor." South Africa.
@misc{etde_7000059,
title = {B-cell stimulating factor}
author = {Clevenger, W R, Conlon, P J, Eisenman, J R, Gillis, S, Grabstein, K H, Hopp, T P, March, C J, Mochizuki, D Y, Price, V L, and Shanebeck, K D}
abstractNote = {BSF-1 was derived from malignant cells and purified by use of various techniques, including adsorption, ion exchange chromatography and reverse phase high performance liquid chromatography. By these techniques, the BSF-1 was purified to homogenity. The high purification of the BSF-1 has made possible the sequencing of the amino acid residues at the N-terminal portion of its protein molecules. From the amino acid sequencing information, a radiolabelled oligonucleotide probe corresponding to portion of the amino acid sequence of the BSF-1 molecule was synthesized and then used to probe a cDNA library prepared from polyadenylated mRNA extracted from cell lines known to produce BSF-1. Through this procedure, a cDNA clone containing the BSF-1 gene was isolated, sequenced and mature BSF-1 expressed. The isolated cDNA clone was then radiolabelled and used as a large probe for screening cDNA libraries of other species of animals for homologous BSF-1 clones.}
place = {South Africa}
year = {1987}
month = {Oct}
}