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Effect of L-glutamate on the metabolic recovery of the myocardium after K sup (+)- induced cardioplegic arrest. Die effek van L-glutamaat op die metaboliese herstel van die miokard na K sup (+)-geinduseerde kardioplegiese arres

Thesis/Dissertation:

Abstract

A study was undertaken to determine the effect of L-glutamate on the metabolic recovery of the myocardium after K sup (+)-induced cardioplegic arrest. Isolated rat hearts and intact dog hearts were subjected to a K sup (+)-induced cardioplegic period of 60 minutes. Preservation temperature was kept constant at 26 degrees Celsius during the entire cardioplegic period. Different concentrations of L-glutamate were added to a dose of secondary cardioplegia. Several parameters, such as isotonic contraction, left ventricular function, enzymatic changes, coronary vascular resistance (coronary flow), oedema, re-animation time-interval and lactate-release were used to determine the above mentioned effect. Radioactive L-glutamate was used to determine the quantitative uptake and distribution of L-glutamate in the myocardium. Basic spectrophotometric methods were used to determine the concentrations of creatine phosphokinase, lactic dehydrogenase and lactate. Standard differential separation methods were used to divide myocardial samples into the different tissue and cytologic fractions. Biophysical methods were used to measure radioactivity in the respective tissue and cytologic fractions. Experimental results were statistically analyzed according to analysis of variance and at-test (Tukey's Test). Preliminary findings stress the important role of L-glutamate in the metabolic recovery of the myocardium after K sup (+)-induced arrest and consequently the positive contribution in  More>>
Publication Date:
Jan 01, 1984
Product Type:
Thesis/Dissertation
Reference Number:
AIX-18-005736; EDB-87-025268
Resource Relation:
Other Information: Thesis (Ph.D.). Include 6 pages of plates
Subject:
59 BASIC BIOLOGICAL SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; GLUTAMIC ACID; TISSUE DISTRIBUTION; MYOCARDIUM; METABOLISM; CARBON 14 COMPOUNDS; CONTRACTION; CORONARIES; DOGS; EDEMA; ENZYME ACTIVITY; ISCHEMIA; LACTATES; MITOCHONDRIA; OXIDOREDUCTASES; PATHOLOGICAL CHANGES; PERFUSED ORGANS; PHOSPHOTRANSFERASES; POTASSIUM; RATS; SUBCELLULAR DISTRIBUTION; TRACER TECHNIQUES; UPTAKE; ALKALI METALS; AMINO ACIDS; ANIMALS; ARTERIES; BLOOD VESSELS; BODY; CARBOXYLIC ACID SALTS; CARBOXYLIC ACIDS; CARDIOVASCULAR DISEASES; CARDIOVASCULAR SYSTEM; CELL CONSTITUENTS; DISEASES; DISTRIBUTION; ELEMENTS; ENZYMES; HEART; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MAMMALS; METALS; MUSCLES; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANOIDS; ORGANS; PHOSPHORUS-GROUP TRANSFERASES; RODENTS; SYMPTOMS; TRANSFERASES; VASCULAR DISEASES; VERTEBRATES; 550501* - Metabolism- Tracer Techniques; 560400 - Other Environmental Pollutant Effects
OSTI ID:
6958180
Research Organizations:
University of the Orange Free State, Bloemfontein (South Africa)
Country of Origin:
South Africa
Language:
Afrikaans
Availability:
Registrar, University of the Orange Free State, P.O. Box 339, Bloemfontein, 9300, South Africa.
Submitting Site:
INIS
Size:
Pages: 176
Announcement Date:

Thesis/Dissertation:

Citation Formats

Neethling, W M.L. Effect of L-glutamate on the metabolic recovery of the myocardium after K sup (+)- induced cardioplegic arrest. Die effek van L-glutamaat op die metaboliese herstel van die miokard na K sup (+)-geinduseerde kardioplegiese arres. South Africa: N. p., 1984. Web.
Neethling, W M.L. Effect of L-glutamate on the metabolic recovery of the myocardium after K sup (+)- induced cardioplegic arrest. Die effek van L-glutamaat op die metaboliese herstel van die miokard na K sup (+)-geinduseerde kardioplegiese arres. South Africa.
Neethling, W M.L. 1984. "Effect of L-glutamate on the metabolic recovery of the myocardium after K sup (+)- induced cardioplegic arrest. Die effek van L-glutamaat op die metaboliese herstel van die miokard na K sup (+)-geinduseerde kardioplegiese arres." South Africa.
@misc{etde_6958180,
title = {Effect of L-glutamate on the metabolic recovery of the myocardium after K sup (+)- induced cardioplegic arrest. Die effek van L-glutamaat op die metaboliese herstel van die miokard na K sup (+)-geinduseerde kardioplegiese arres}
author = {Neethling, W M.L.}
abstractNote = {A study was undertaken to determine the effect of L-glutamate on the metabolic recovery of the myocardium after K sup (+)-induced cardioplegic arrest. Isolated rat hearts and intact dog hearts were subjected to a K sup (+)-induced cardioplegic period of 60 minutes. Preservation temperature was kept constant at 26 degrees Celsius during the entire cardioplegic period. Different concentrations of L-glutamate were added to a dose of secondary cardioplegia. Several parameters, such as isotonic contraction, left ventricular function, enzymatic changes, coronary vascular resistance (coronary flow), oedema, re-animation time-interval and lactate-release were used to determine the above mentioned effect. Radioactive L-glutamate was used to determine the quantitative uptake and distribution of L-glutamate in the myocardium. Basic spectrophotometric methods were used to determine the concentrations of creatine phosphokinase, lactic dehydrogenase and lactate. Standard differential separation methods were used to divide myocardial samples into the different tissue and cytologic fractions. Biophysical methods were used to measure radioactivity in the respective tissue and cytologic fractions. Experimental results were statistically analyzed according to analysis of variance and at-test (Tukey's Test). Preliminary findings stress the important role of L-glutamate in the metabolic recovery of the myocardium after K sup (+)-induced arrest and consequently the positive contribution in the search for the best method of myocardial preservation during cardiac surgery.}
place = {South Africa}
year = {1984}
month = {Jan}
}