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Radioimmunoassay of creatine kinase BB isoenzyme

Conference:

Abstract

A radioimmunoassay of creatine kinase BB isoenzyme (CK-BB) was developed by using CK-BB purified from human brain. The CK-BB antiserum was raised by immunizing rabbite and {sup 125}I-CK-BB iodinated with Bolton-Hunter reagent. The affinity constant was 3.0 x 10{sup 9} mol/L. No cross reactions with creatine kinase MM isoenzyme and neuron-specific enolase were found. The measuring range was 3.5 x 10{sup -8} {approx} 1.2 x 10{sup -5} mmol/L, the average recovery rate 97.5%, with the inter and intrassay CV 3.1% and 12%, respectively. The average serum CK-BB concentration in 83 normal persons was 1.5 x 10{sup -7} +- 8.1 x 10{sup -8} mmol/L, quite different from the values of acute myocardial infarction (5.2 x 10{sup -6} +- 1.2 x 10{sup -4} mmol/L, n = 28) and cerebral vascular accident (8.4 x 10{sup -4} +- 5.0 x 10{sup -4} mmol/L, n = 10).
Authors:
Jianguo, Geng [1] 
  1. Shanghai Medical Univ. (China). Zhongshan Hospital; and others
Publication Date:
Nov 01, 1988
Product Type:
Conference
Report Number:
CONF-881050-
Reference Number:
AIX-21-043004; EDB-90-081322
Resource Relation:
Journal Name: Chinese Journal of Nuclear Medicine (Zhonghua Heyixue Zazhi); (China); Journal Volume: 8:4; Conference: International Symposium on Nuclear Medicine (ISNM'88), Beijing (China), 10-14 Oct 1988
Subject:
60 APPLIED LIFE SCIENCES; ISOENZYMES; RADIOIMMUNOASSAY; ANTIBODIES; BLOOD SERUM; CARDIOVASCULAR DISEASES; CREATINE; IODINE 125; LABELLED COMPOUNDS; PHOSPHOTRANSFERASES; AMINO ACIDS; BETA DECAY RADIOISOTOPES; BIOASSAY; CARBOXYLIC ACIDS; DAYS LIVING RADIOISOTOPES; DIAGNOSTIC TECHNIQUES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; ENZYMES; IMMUNOASSAY; IMMUNOLOGY; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; PHOSPHORUS-GROUP TRANSFERASES; RADIOASSAY; RADIOIMMUNODETECTION; RADIOIMMUNOLOGY; RADIOISOTOPES; TRACER TECHNIQUES; TRANSFERASES; 553006* - Agriculture & Food Technology- Other Agricultural Applications- (1987-)
OSTI ID:
6957279
Country of Origin:
China
Language:
Chinese
Other Identifying Numbers:
Journal ID: ISSN 0253-9780; CODEN: CITCD
Submitting Site:
INIS
Size:
Pages: 226-228
Announcement Date:
May 13, 2001

Conference:

Citation Formats

Jianguo, Geng. Radioimmunoassay of creatine kinase BB isoenzyme. China: N. p., 1988. Web.
Jianguo, Geng. Radioimmunoassay of creatine kinase BB isoenzyme. China.
Jianguo, Geng. 1988. "Radioimmunoassay of creatine kinase BB isoenzyme." China.
@misc{etde_6957279,
title = {Radioimmunoassay of creatine kinase BB isoenzyme}
author = {Jianguo, Geng}
abstractNote = {A radioimmunoassay of creatine kinase BB isoenzyme (CK-BB) was developed by using CK-BB purified from human brain. The CK-BB antiserum was raised by immunizing rabbite and {sup 125}I-CK-BB iodinated with Bolton-Hunter reagent. The affinity constant was 3.0 x 10{sup 9} mol/L. No cross reactions with creatine kinase MM isoenzyme and neuron-specific enolase were found. The measuring range was 3.5 x 10{sup -8} {approx} 1.2 x 10{sup -5} mmol/L, the average recovery rate 97.5%, with the inter and intrassay CV 3.1% and 12%, respectively. The average serum CK-BB concentration in 83 normal persons was 1.5 x 10{sup -7} +- 8.1 x 10{sup -8} mmol/L, quite different from the values of acute myocardial infarction (5.2 x 10{sup -6} +- 1.2 x 10{sup -4} mmol/L, n = 28) and cerebral vascular accident (8.4 x 10{sup -4} +- 5.0 x 10{sup -4} mmol/L, n = 10).}
journal = {Chinese Journal of Nuclear Medicine (Zhonghua Heyixue Zazhi); (China)}
volume = {8:4}
place = {China}
year = {1988}
month = {Nov}
}