Abstract
The authors have previously constructed a cDNA clone pHCAL1, covering most of the C-terminal propeptide domain of human pro..cap alpha..1(I) collagen mRNA,by inserting a 678 bp EcoRI-XhoI fragment of cDNA into pBR322. Since the XhoI/SalI ligation prevented removal of the insert, they used the same strategy to obtain a similar clone in pUC8. RNA was isolated from fetal calvarial bones. The cDNA was digested with EcoRI and XhoI and fractionated on a 1 % agarose gel. Fragments of 650-700 bp were cloned in pUC8 at the polylinker site, which now permits easy removal of the insert. The new clone was named pHCAL1U since the RNA was isolated from another individual. The approach outlined is useful for studies on individual variation which is important to recognize when searching for disease-related mutations in type I collagen.
Citation Formats
Maekelae, J K, Raassina, M, Virta, A, and Vuorio, E.
Human pro. cap alpha. 1)(I) collagen: cDNA sequence for the C-propeptide domain.
United Kingdom: N. p.,
1988.
Web.
doi:10.1093/nar/16.1.349.
Maekelae, J K, Raassina, M, Virta, A, & Vuorio, E.
Human pro. cap alpha. 1)(I) collagen: cDNA sequence for the C-propeptide domain.
United Kingdom.
https://doi.org/10.1093/nar/16.1.349
Maekelae, J K, Raassina, M, Virta, A, and Vuorio, E.
1988.
"Human pro. cap alpha. 1)(I) collagen: cDNA sequence for the C-propeptide domain."
United Kingdom.
https://doi.org/10.1093/nar/16.1.349.
@misc{etde_6957051,
title = {Human pro. cap alpha. 1)(I) collagen: cDNA sequence for the C-propeptide domain}
author = {Maekelae, J K, Raassina, M, Virta, A, and Vuorio, E}
abstractNote = {The authors have previously constructed a cDNA clone pHCAL1, covering most of the C-terminal propeptide domain of human pro..cap alpha..1(I) collagen mRNA,by inserting a 678 bp EcoRI-XhoI fragment of cDNA into pBR322. Since the XhoI/SalI ligation prevented removal of the insert, they used the same strategy to obtain a similar clone in pUC8. RNA was isolated from fetal calvarial bones. The cDNA was digested with EcoRI and XhoI and fractionated on a 1 % agarose gel. Fragments of 650-700 bp were cloned in pUC8 at the polylinker site, which now permits easy removal of the insert. The new clone was named pHCAL1U since the RNA was isolated from another individual. The approach outlined is useful for studies on individual variation which is important to recognize when searching for disease-related mutations in type I collagen.}
doi = {10.1093/nar/16.1.349}
journal = []
volume = {16:1}
journal type = {AC}
place = {United Kingdom}
year = {1988}
month = {Jan}
}
title = {Human pro. cap alpha. 1)(I) collagen: cDNA sequence for the C-propeptide domain}
author = {Maekelae, J K, Raassina, M, Virta, A, and Vuorio, E}
abstractNote = {The authors have previously constructed a cDNA clone pHCAL1, covering most of the C-terminal propeptide domain of human pro..cap alpha..1(I) collagen mRNA,by inserting a 678 bp EcoRI-XhoI fragment of cDNA into pBR322. Since the XhoI/SalI ligation prevented removal of the insert, they used the same strategy to obtain a similar clone in pUC8. RNA was isolated from fetal calvarial bones. The cDNA was digested with EcoRI and XhoI and fractionated on a 1 % agarose gel. Fragments of 650-700 bp were cloned in pUC8 at the polylinker site, which now permits easy removal of the insert. The new clone was named pHCAL1U since the RNA was isolated from another individual. The approach outlined is useful for studies on individual variation which is important to recognize when searching for disease-related mutations in type I collagen.}
doi = {10.1093/nar/16.1.349}
journal = []
volume = {16:1}
journal type = {AC}
place = {United Kingdom}
year = {1988}
month = {Jan}
}