Abstract
Calbindin D28K (formerly Vitamin D-Dependent Calcium Binding Protein) is a protein induced by 1,25-dihydroxycholecalciferol in several chicken tissues. A chicken genomic DNA library was screened with a synthetic oligonucleotide representing the sequence of Calbindin D18K cDNA from nt 146 to nt 176. The positive clone CBAl extends the 5'-end of the first exon by 451 bp. The sequence of a BamHI-SacII restriction fragment with coordinates -451 + 50 is shown. The BamHI-SacII fragment was subcloned 5' to the CAT gene of pUCCAT. The result is shown of a CAT assay on mouse fibroblasts 3T6 transiently transfected with pUCCAT, pUCCAT containing the BamHI-SacII fragment in the correct or opposite orientation or the SV40 promoter. /sup 14/C-chloramphenicol and its acetyl derivatives generated by purified CAT are also shown. The expression of CAT appears to be constitutive since the enzyme activity is not influenced by the presence (+) or absence (-) of 1,25-dihydroxycholecalciferol in the culture medium.
Citation Formats
Ferrari, S, Drusiani, E, Battini, R, and Fregni, M.
Nucleotide sequence of the promoter region of the gene encoding chicken Calbindin D28K.
United Kingdom: N. p.,
1988.
Web.
doi:10.1093/nar/16.1.353.
Ferrari, S, Drusiani, E, Battini, R, & Fregni, M.
Nucleotide sequence of the promoter region of the gene encoding chicken Calbindin D28K.
United Kingdom.
https://doi.org/10.1093/nar/16.1.353
Ferrari, S, Drusiani, E, Battini, R, and Fregni, M.
1988.
"Nucleotide sequence of the promoter region of the gene encoding chicken Calbindin D28K."
United Kingdom.
https://doi.org/10.1093/nar/16.1.353.
@misc{etde_6956762,
title = {Nucleotide sequence of the promoter region of the gene encoding chicken Calbindin D28K}
author = {Ferrari, S, Drusiani, E, Battini, R, and Fregni, M}
abstractNote = {Calbindin D28K (formerly Vitamin D-Dependent Calcium Binding Protein) is a protein induced by 1,25-dihydroxycholecalciferol in several chicken tissues. A chicken genomic DNA library was screened with a synthetic oligonucleotide representing the sequence of Calbindin D18K cDNA from nt 146 to nt 176. The positive clone CBAl extends the 5'-end of the first exon by 451 bp. The sequence of a BamHI-SacII restriction fragment with coordinates -451 + 50 is shown. The BamHI-SacII fragment was subcloned 5' to the CAT gene of pUCCAT. The result is shown of a CAT assay on mouse fibroblasts 3T6 transiently transfected with pUCCAT, pUCCAT containing the BamHI-SacII fragment in the correct or opposite orientation or the SV40 promoter. /sup 14/C-chloramphenicol and its acetyl derivatives generated by purified CAT are also shown. The expression of CAT appears to be constitutive since the enzyme activity is not influenced by the presence (+) or absence (-) of 1,25-dihydroxycholecalciferol in the culture medium.}
doi = {10.1093/nar/16.1.353}
journal = []
volume = {16:1}
journal type = {AC}
place = {United Kingdom}
year = {1988}
month = {Jan}
}
title = {Nucleotide sequence of the promoter region of the gene encoding chicken Calbindin D28K}
author = {Ferrari, S, Drusiani, E, Battini, R, and Fregni, M}
abstractNote = {Calbindin D28K (formerly Vitamin D-Dependent Calcium Binding Protein) is a protein induced by 1,25-dihydroxycholecalciferol in several chicken tissues. A chicken genomic DNA library was screened with a synthetic oligonucleotide representing the sequence of Calbindin D18K cDNA from nt 146 to nt 176. The positive clone CBAl extends the 5'-end of the first exon by 451 bp. The sequence of a BamHI-SacII restriction fragment with coordinates -451 + 50 is shown. The BamHI-SacII fragment was subcloned 5' to the CAT gene of pUCCAT. The result is shown of a CAT assay on mouse fibroblasts 3T6 transiently transfected with pUCCAT, pUCCAT containing the BamHI-SacII fragment in the correct or opposite orientation or the SV40 promoter. /sup 14/C-chloramphenicol and its acetyl derivatives generated by purified CAT are also shown. The expression of CAT appears to be constitutive since the enzyme activity is not influenced by the presence (+) or absence (-) of 1,25-dihydroxycholecalciferol in the culture medium.}
doi = {10.1093/nar/16.1.353}
journal = []
volume = {16:1}
journal type = {AC}
place = {United Kingdom}
year = {1988}
month = {Jan}
}