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Application of genetically engineered microorganisms to bioremediation and wastewater treatment. Idenshi sosa biseibutsu no kankyo joka mizushori eno tekiyo

Journal Article:

Abstract

This paper summarizes the following techniques: a gene engineering method for bioremediation and wastewater treatment, microorganism breeding using the former method, and a monitoring technique for genetical and ecological stability of genetically engineered microorganisms. Recombination bacteria reinforced with PH genes showed higher phenol removing rate than wild strains, but presented accumulation of catechol in such a large quantity as cannot be seen in wild strains, with the complete degradation rate rather decreased. Gene recombined bacteria structured by introducing the recombined plasmid, pBH500, had high genetic stability when P.putida BH-1 is used as a host. E.coli C600 having recombined plasmid and P.putida BH were added and cultivated in activated sludge. As a result, both recombined bacteria showed rapid logarithmic decrease just after the addition, then, maintained the relatively stable population groups, and remained in the activated sludge for an extended period of time. In monitoring techniques, the colony hybridization process detected clearly the gene recombined bacteria. 9 refs., 7 figs., 1 tab.
Authors:
Fujita, M; Ike, M [1] 
  1. Osaka University, Osaka (Japan). Faculty of Engineering
Publication Date:
Nov 10, 1993
Product Type:
Journal Article
Reference Number:
NEDO-93-914407; EDB-94-141983
Resource Relation:
Journal Name: Mizu Kankyo Gakkaishi (Journal of Japan Society on Water Environment); (Japan); Journal Volume: 16:11
Subject:
09 BIOMASS FUELS; 54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; MICROORGANISMS; GENETIC ENGINEERING; PHENOLS; BIODEGRADATION; SEWAGE SLUDGE; ACTIVATED SLUDGE PROCESS; BIOTECHNOLOGY; CLEANING; DNA HYBRIDIZATION; GENE RECOMBINATION; MONITORING; PLASMIDS; WASTE WATER; WATER TREATMENT; AROMATICS; BIOLOGICAL MATERIALS; BIOLOGICAL WASTES; CELL CONSTITUENTS; CHEMICAL REACTIONS; DECOMPOSITION; HYBRIDIZATION; HYDROGEN COMPOUNDS; HYDROXY COMPOUNDS; LIQUID WASTES; MANAGEMENT; MATERIALS; ORGANIC COMPOUNDS; OXYGEN COMPOUNDS; PROCESSING; SEWAGE; SLUDGES; WASTE MANAGEMENT; WASTE PROCESSING; WASTES; WATER; 090900* - Biomass Fuels- Processing- (1990-); 540320 - Environment, Aquatic- Chemicals Monitoring & Transport- (1990-); 550400 - Genetics
OSTI ID:
6945599
Country of Origin:
Japan
Language:
Japanese
Other Identifying Numbers:
Journal ID: ISSN 0916-8958; CODEN: MKGAEY
Submitting Site:
NEDO
Size:
Pages: 2-9
Announcement Date:

Journal Article:

Citation Formats

Fujita, M, and Ike, M. Application of genetically engineered microorganisms to bioremediation and wastewater treatment. Idenshi sosa biseibutsu no kankyo joka mizushori eno tekiyo. Japan: N. p., 1993. Web.
Fujita, M, & Ike, M. Application of genetically engineered microorganisms to bioremediation and wastewater treatment. Idenshi sosa biseibutsu no kankyo joka mizushori eno tekiyo. Japan.
Fujita, M, and Ike, M. 1993. "Application of genetically engineered microorganisms to bioremediation and wastewater treatment. Idenshi sosa biseibutsu no kankyo joka mizushori eno tekiyo." Japan.
@misc{etde_6945599,
title = {Application of genetically engineered microorganisms to bioremediation and wastewater treatment. Idenshi sosa biseibutsu no kankyo joka mizushori eno tekiyo}
author = {Fujita, M, and Ike, M}
abstractNote = {This paper summarizes the following techniques: a gene engineering method for bioremediation and wastewater treatment, microorganism breeding using the former method, and a monitoring technique for genetical and ecological stability of genetically engineered microorganisms. Recombination bacteria reinforced with PH genes showed higher phenol removing rate than wild strains, but presented accumulation of catechol in such a large quantity as cannot be seen in wild strains, with the complete degradation rate rather decreased. Gene recombined bacteria structured by introducing the recombined plasmid, pBH500, had high genetic stability when P.putida BH-1 is used as a host. E.coli C600 having recombined plasmid and P.putida BH were added and cultivated in activated sludge. As a result, both recombined bacteria showed rapid logarithmic decrease just after the addition, then, maintained the relatively stable population groups, and remained in the activated sludge for an extended period of time. In monitoring techniques, the colony hybridization process detected clearly the gene recombined bacteria. 9 refs., 7 figs., 1 tab.}
journal = {Mizu Kankyo Gakkaishi (Journal of Japan Society on Water Environment); (Japan)}
volume = {16:11}
journal type = {AC}
place = {Japan}
year = {1993}
month = {Nov}
}