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Structural studies on reaction centers from thermophilic photosynthetic bacteria and its functional utilizations. Tainetsusei kogosei saikin ni yuraisuru kogosei hanno chushin no kozo kaimei to kino kaihatsu

Journal Article:

Abstract

This paper describes the results of the experiment in which crystallization of protein of reactive center purified from the photosynthetic film of thermophilic purple sulfur photosynthetic bacterium Chromatium tepidum whose hyrogen donor in photosynthesis is H2S instead of H2O was attempted. Crystallization was carried out by the vapor diffusion method and particularly by using ethylene glycol as precipitator at 4[degree]C after various investigations on the conditions of crystallization. By X-ray diffraction, this crystal was found to belong to the rhombic system, and it was estimated that the lattice constants, a, b, c equal to 140[angstrom], 190[angstrom] and 80[angstrom] respectively. This bacterium is a thermophilic bacterium having the optimum growth temperature of 48-50 [degree]C and utilizes CO2 or H2CO3 as corbon source, ammonium, urea etc. as nitrogen source and thiosulfate as sulfur source. Moreover, another purpose of this investigation was to determine the thermophilic location by elucidating its configuration (although, as a result, the analysis of configuration had no sufficient resolution). It was confirmed that the enzyme system of photosynthetic film and its cytoplasm obtained by ultrasonic spallation of this cell have CO2 fixing activity utilizing light energy. 23 refs., 14 figs., 3 tabs.
Authors:
Nozawa, T; Morishita, Y; Kobayashi, M; Kanno, S [1] 
  1. Tohoku University, Sendai (Japan). Faculty of Engineering
Publication Date:
Oct 31, 1992
Product Type:
Journal Article
Reference Number:
NEDO-92-950554; EDB-93-055245
Resource Relation:
Journal Name: Asahi Garasu Zaidan Kenkyu Hokoku; (Japan); Journal Volume: 60
Subject:
59 BASIC BIOLOGICAL SCIENCES; 14 SOLAR ENERGY; HYDROGEN SULFIDES; HYDROGEN TRANSFER; PHOTOSYNTHETIC BACTERIA; PHOTOSYNTHETIC REACTION CENTERS; SULFUR-OXIDIZING BACTERIA; THERMOPHILIC CONDITIONS; CRYSTALLIZATION; CYTOPLASM; ENZYMES; GASEOUS DIFFUSION; PHOTOSYNTHETIC MEMBRANES; PRECIPITATION; PROTEINS; STRUCTURAL CHEMICAL ANALYSIS; CARBON DIOXIDE FIXATION; COMMINUTION; ULTRASONIC WAVES; BACTERIA; CELL CONSTITUENTS; CHALCOGENIDES; DIFFUSION; HYDROGEN COMPOUNDS; MEMBRANES; MICROORGANISMS; ORGANIC COMPOUNDS; PHASE TRANSFORMATIONS; SEPARATION PROCESSES; SOUND WAVES; SULFIDES; SULFUR COMPOUNDS; 550700* - Microbiology; 550300 - Cytology; 140505 - Solar Energy Conversion- Photochemical, Photobiological, & Thermochemical Conversion- (1980-)
OSTI ID:
6944242
Country of Origin:
Japan
Language:
Japanese
Other Identifying Numbers:
Journal ID: ISSN 0916-7064; CODEN: KHAZE2
Submitting Site:
NEDO
Size:
Pages: 107-118
Announcement Date:

Journal Article:

Citation Formats

Nozawa, T, Morishita, Y, Kobayashi, M, and Kanno, S. Structural studies on reaction centers from thermophilic photosynthetic bacteria and its functional utilizations. Tainetsusei kogosei saikin ni yuraisuru kogosei hanno chushin no kozo kaimei to kino kaihatsu. Japan: N. p., 1992. Web.
Nozawa, T, Morishita, Y, Kobayashi, M, & Kanno, S. Structural studies on reaction centers from thermophilic photosynthetic bacteria and its functional utilizations. Tainetsusei kogosei saikin ni yuraisuru kogosei hanno chushin no kozo kaimei to kino kaihatsu. Japan.
Nozawa, T, Morishita, Y, Kobayashi, M, and Kanno, S. 1992. "Structural studies on reaction centers from thermophilic photosynthetic bacteria and its functional utilizations. Tainetsusei kogosei saikin ni yuraisuru kogosei hanno chushin no kozo kaimei to kino kaihatsu." Japan.
@misc{etde_6944242,
title = {Structural studies on reaction centers from thermophilic photosynthetic bacteria and its functional utilizations. Tainetsusei kogosei saikin ni yuraisuru kogosei hanno chushin no kozo kaimei to kino kaihatsu}
author = {Nozawa, T, Morishita, Y, Kobayashi, M, and Kanno, S}
abstractNote = {This paper describes the results of the experiment in which crystallization of protein of reactive center purified from the photosynthetic film of thermophilic purple sulfur photosynthetic bacterium Chromatium tepidum whose hyrogen donor in photosynthesis is H2S instead of H2O was attempted. Crystallization was carried out by the vapor diffusion method and particularly by using ethylene glycol as precipitator at 4[degree]C after various investigations on the conditions of crystallization. By X-ray diffraction, this crystal was found to belong to the rhombic system, and it was estimated that the lattice constants, a, b, c equal to 140[angstrom], 190[angstrom] and 80[angstrom] respectively. This bacterium is a thermophilic bacterium having the optimum growth temperature of 48-50 [degree]C and utilizes CO2 or H2CO3 as corbon source, ammonium, urea etc. as nitrogen source and thiosulfate as sulfur source. Moreover, another purpose of this investigation was to determine the thermophilic location by elucidating its configuration (although, as a result, the analysis of configuration had no sufficient resolution). It was confirmed that the enzyme system of photosynthetic film and its cytoplasm obtained by ultrasonic spallation of this cell have CO2 fixing activity utilizing light energy. 23 refs., 14 figs., 3 tabs.}
journal = {Asahi Garasu Zaidan Kenkyu Hokoku; (Japan)}
volume = {60}
journal type = {AC}
place = {Japan}
year = {1992}
month = {Oct}
}