Abstract
Exchange of two homologous DNA helices between sister chromatids can be graphically visualized in metaphase chromosomes by differential staining of chromatids. This ubiquitous phenomenon, SCE, represents an example of dynamic behavior of the genetic material. SCEs have given the great impact to those interested in not only the basic mechanism of DNA recombination but also environmental mutagenesis. This article overviews the accumulated experimental data on SCE formation, highlighting the methodology for SCE detection, induction of SCEs in vitro and in vivo, radiation-induced SCEs, the mechanistic aspects of SCE formation, SCE and DNA methylation, SCE and chromosomal aberration, and adaptive chromosomal DNA repair revealed by SCE studies. A biological role of SCE formation is considered to be the basic mechanism for generating genetic variation through gene magnification, rearrangement and amplification in the cells during the course of ontogenical and phylogenical differentiation. (author).
Ikushima, Takaji
[1]
- Kyoto Univ., Kumatori, Osaka (Japan). Research Reactor Inst.
Citation Formats
Ikushima, Takaji.
SCE enigma; Methodology, mechanism and meaning of sister chromatid exchange.
Japan: N. p.,
1989.
Web.
Ikushima, Takaji.
SCE enigma; Methodology, mechanism and meaning of sister chromatid exchange.
Japan.
Ikushima, Takaji.
1989.
"SCE enigma; Methodology, mechanism and meaning of sister chromatid exchange."
Japan.
@misc{etde_6937261,
title = {SCE enigma; Methodology, mechanism and meaning of sister chromatid exchange}
author = {Ikushima, Takaji}
abstractNote = {Exchange of two homologous DNA helices between sister chromatids can be graphically visualized in metaphase chromosomes by differential staining of chromatids. This ubiquitous phenomenon, SCE, represents an example of dynamic behavior of the genetic material. SCEs have given the great impact to those interested in not only the basic mechanism of DNA recombination but also environmental mutagenesis. This article overviews the accumulated experimental data on SCE formation, highlighting the methodology for SCE detection, induction of SCEs in vitro and in vivo, radiation-induced SCEs, the mechanistic aspects of SCE formation, SCE and DNA methylation, SCE and chromosomal aberration, and adaptive chromosomal DNA repair revealed by SCE studies. A biological role of SCE formation is considered to be the basic mechanism for generating genetic variation through gene magnification, rearrangement and amplification in the cells during the course of ontogenical and phylogenical differentiation. (author).}
journal = []
volume = {22}
journal type = {AC}
place = {Japan}
year = {1989}
month = {Nov}
}
title = {SCE enigma; Methodology, mechanism and meaning of sister chromatid exchange}
author = {Ikushima, Takaji}
abstractNote = {Exchange of two homologous DNA helices between sister chromatids can be graphically visualized in metaphase chromosomes by differential staining of chromatids. This ubiquitous phenomenon, SCE, represents an example of dynamic behavior of the genetic material. SCEs have given the great impact to those interested in not only the basic mechanism of DNA recombination but also environmental mutagenesis. This article overviews the accumulated experimental data on SCE formation, highlighting the methodology for SCE detection, induction of SCEs in vitro and in vivo, radiation-induced SCEs, the mechanistic aspects of SCE formation, SCE and DNA methylation, SCE and chromosomal aberration, and adaptive chromosomal DNA repair revealed by SCE studies. A biological role of SCE formation is considered to be the basic mechanism for generating genetic variation through gene magnification, rearrangement and amplification in the cells during the course of ontogenical and phylogenical differentiation. (author).}
journal = []
volume = {22}
journal type = {AC}
place = {Japan}
year = {1989}
month = {Nov}
}