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Pyrimidine dimer sites associated with the daughter DNA strands in uv-irradiated human fibroblasts

Journal Article:

Abstract

Pyrimidine dimer sites associated with the newly-synthesized DNA were detected during post-replication repair of DNA in uv-irradiated human fibroblasts. These pyrimidine dimer sites were inferred from a decrease in the molecular weight of pulse-labelled DNA after treatment with an extract of Micrococcus luteus containing uv-specific endonuclease activity. In DNA synthesized immediately after irradiation, the frequency of these daughter strand dimer sites was 7 to 20% of that in the parental DNA. Such sites were found in fibroblasts from normal donors and from xeroderma pigmentosum patients (with defects in excision-repair or post-replication repair). They were excised from the DNA of normal cells. As the time between uv irradiation and pulse-labelling was increased, the frequency of dimer sites associated with the labelled DNA decreased. If the pulse-label was delivered 6 h after irradiation of normal cells or excision-defective xeroderma pigmentosum cells, no dimer sites were detected in the labelled DNA. It has usually been assumed that daughter-strand dimer sites were the result of recombinational exchanges. The assay procedure used in these experiments and in similar experiments of others did not distinguish between labelled DNA containing pyrimidine dimers within the labelled section, and labelled DNA which did not contain pyrimidine dimers but was  More>>
Authors:
Lehmann, A R; Kirk-Bell, S [1] 
  1. Sussex Univ., Brighton (UK)
Publication Date:
Mar 01, 1978
Product Type:
Journal Article
Reference Number:
AIX-09-398195; EDB-78-125588
Resource Relation:
Journal Name: Photochem. Photobiol.; (United Kingdom); Journal Volume: 27:3
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DIMERS; DNA REPLICATION; DNA; BIOLOGICAL REPAIR; FIBROBLASTS; BIOLOGICAL RADIATION EFFECTS; PYRIMIDINES; IRRADIATION; LABELLING; PULSES; ULTRAVIOLET RADIATION; XP CELLS; ANIMAL CELLS; AZINES; BIOLOGICAL EFFECTS; BIOLOGICAL RECOVERY; CONNECTIVE TISSUE CELLS; ELECTROMAGNETIC RADIATION; HETEROCYCLIC COMPOUNDS; NUCLEIC ACID REPLICATION; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; RADIATION EFFECTS; RADIATIONS; RECOVERY; REPAIR; SOMATIC CELLS; 560115* - Radiation Effects on Biochemicals- In Man- (-1987); 560151 - Radiation Effects on Animals- Man
OSTI ID:
6929817
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: PHCBA
Submitting Site:
INIS
Size:
Pages: 297-307
Announcement Date:

Journal Article:

Citation Formats

Lehmann, A R, and Kirk-Bell, S. Pyrimidine dimer sites associated with the daughter DNA strands in uv-irradiated human fibroblasts. United Kingdom: N. p., 1978. Web.
Lehmann, A R, & Kirk-Bell, S. Pyrimidine dimer sites associated with the daughter DNA strands in uv-irradiated human fibroblasts. United Kingdom.
Lehmann, A R, and Kirk-Bell, S. 1978. "Pyrimidine dimer sites associated with the daughter DNA strands in uv-irradiated human fibroblasts." United Kingdom.
@misc{etde_6929817,
title = {Pyrimidine dimer sites associated with the daughter DNA strands in uv-irradiated human fibroblasts}
author = {Lehmann, A R, and Kirk-Bell, S}
abstractNote = {Pyrimidine dimer sites associated with the newly-synthesized DNA were detected during post-replication repair of DNA in uv-irradiated human fibroblasts. These pyrimidine dimer sites were inferred from a decrease in the molecular weight of pulse-labelled DNA after treatment with an extract of Micrococcus luteus containing uv-specific endonuclease activity. In DNA synthesized immediately after irradiation, the frequency of these daughter strand dimer sites was 7 to 20% of that in the parental DNA. Such sites were found in fibroblasts from normal donors and from xeroderma pigmentosum patients (with defects in excision-repair or post-replication repair). They were excised from the DNA of normal cells. As the time between uv irradiation and pulse-labelling was increased, the frequency of dimer sites associated with the labelled DNA decreased. If the pulse-label was delivered 6 h after irradiation of normal cells or excision-defective xeroderma pigmentosum cells, no dimer sites were detected in the labelled DNA. It has usually been assumed that daughter-strand dimer sites were the result of recombinational exchanges. The assay procedure used in these experiments and in similar experiments of others did not distinguish between labelled DNA containing pyrimidine dimers within the labelled section, and labelled DNA which did not contain pyrimidine dimers but was attached to unlabelled DNA which did contain dimers. The latter structures would arise during normal replication immediately following uv irradiation of mammalian cells. Calculations are presented which suggest that a significant proportion and conceivably all of the dimer sites associated with the daughter strands may have arisen in this way, rather than from recombinational exchanges as has been generally assumed.}
journal = {Photochem. Photobiol.; (United Kingdom)}
volume = {27:3}
journal type = {AC}
place = {United Kingdom}
year = {1978}
month = {Mar}
}