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Stage sensitivity and dose response of meiotic chromosomes of pollen mother cells of Tradescantia to X-rays

Abstract

Chromosome damage induced by physical and chemical mutagens can be quantitated by the frequencies of micronuclei (MCN) produced in tetrads of the meiotic pollen mother cells of Tradescantia, i.e. the 'MCN-in-Tetrad' test. The stage sensitivity and dose response of these meiocytes to low exposures of X-rays was studied to improve the efficiency and reliability of this test. Stage sensitivity was determined by observing, at 3 hr intervals, the frequencies of X-ray (35 rads)-induced MCN in tetrads from a series of 16 fixations of tetrad-containing inflorescences. Late stages of meiosis (3-9 hr post-irradiation fixation groups) were insensitive (5-14 MCN/100 tetrads). Relatively high sensitivity was exhibited in the early stages of meiosis. The first and second sensitive peaks (62 and 61 MCN/100 tetrads) centered around the 21 and 39 hr post-irradiation fixation groups respectively. Control groups yielded around 3-4 MCN/100 tetrads. A dose-response relation for MCN was determined by treating early stages of meiotic pollen mother cells with X-ray exposures ranging from 9.5 to 57.5 rads. A linear regression line was established with about 20 MCN/100 tetrads per 10 rad increment.
Authors:
Ma, T H; Kontos, Jr, G J; Anderson, V A [1] 
  1. Western Illinois Univ., Macomb (USA). Dept. of Biological Sciences
Publication Date:
May 01, 1980
Product Type:
Journal Article
Reference Number:
AIX-11-558440; EDB-81-002405
Resource Relation:
Journal Name: Environ. Exp. Bot.; (United Kingdom); Journal Volume: 20:2
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; TRADESCANTIA; ACUTE IRRADIATION; CELL NUCLEI; CHROMOSOMAL ABERRATIONS; DOSE-RESPONSE RELATIONSHIPS; MEIOSIS; POLLEN; X RADIATION; ACUTE EXPOSURE; CELL CONSTITUENTS; CELL DIVISION; ELECTROMAGNETIC RADIATION; GAMETES; GERM CELLS; IONIZING RADIATIONS; IRRADIATION; MUTATIONS; PLANTS; RADIATIONS; 560141* - Radiation Effects on Plants- Basic Studies- (-1987)
OSTI ID:
6926099
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: EEBOD
Submitting Site:
INIS
Size:
Pages: 169-174
Announcement Date:

Citation Formats

Ma, T H, Kontos, Jr, G J, and Anderson, V A. Stage sensitivity and dose response of meiotic chromosomes of pollen mother cells of Tradescantia to X-rays. United Kingdom: N. p., 1980. Web. doi:10.1016/0098-8472(80)90010-6.
Ma, T H, Kontos, Jr, G J, & Anderson, V A. Stage sensitivity and dose response of meiotic chromosomes of pollen mother cells of Tradescantia to X-rays. United Kingdom. doi:10.1016/0098-8472(80)90010-6.
Ma, T H, Kontos, Jr, G J, and Anderson, V A. 1980. "Stage sensitivity and dose response of meiotic chromosomes of pollen mother cells of Tradescantia to X-rays." United Kingdom. doi:10.1016/0098-8472(80)90010-6. https://www.osti.gov/servlets/purl/10.1016/0098-8472(80)90010-6.
@misc{etde_6926099,
title = {Stage sensitivity and dose response of meiotic chromosomes of pollen mother cells of Tradescantia to X-rays}
author = {Ma, T H, Kontos, Jr, G J, and Anderson, V A}
abstractNote = {Chromosome damage induced by physical and chemical mutagens can be quantitated by the frequencies of micronuclei (MCN) produced in tetrads of the meiotic pollen mother cells of Tradescantia, i.e. the 'MCN-in-Tetrad' test. The stage sensitivity and dose response of these meiocytes to low exposures of X-rays was studied to improve the efficiency and reliability of this test. Stage sensitivity was determined by observing, at 3 hr intervals, the frequencies of X-ray (35 rads)-induced MCN in tetrads from a series of 16 fixations of tetrad-containing inflorescences. Late stages of meiosis (3-9 hr post-irradiation fixation groups) were insensitive (5-14 MCN/100 tetrads). Relatively high sensitivity was exhibited in the early stages of meiosis. The first and second sensitive peaks (62 and 61 MCN/100 tetrads) centered around the 21 and 39 hr post-irradiation fixation groups respectively. Control groups yielded around 3-4 MCN/100 tetrads. A dose-response relation for MCN was determined by treating early stages of meiotic pollen mother cells with X-ray exposures ranging from 9.5 to 57.5 rads. A linear regression line was established with about 20 MCN/100 tetrads per 10 rad increment.}
doi = {10.1016/0098-8472(80)90010-6}
journal = {Environ. Exp. Bot.; (United Kingdom)}
volume = {20:2}
journal type = {AC}
place = {United Kingdom}
year = {1980}
month = {May}
}