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Damage to E. coli cells induced by tritium decay: secondary lethality under nongrowth conditions

Journal Article:

Abstract

Cells containing incorporated /sup 3/H-thymidine are damaged by its decay. It was found with E.coli TAU-bar cells that a small part of the damage is lethal whereas most of it is reparable and only potentially lethal. If cells are subjected to nongrowth conditions, the potentially lethal damage changes to lethal damage. This process is called secondary lethality (SL). The extent of SL and some changes in DNA under three different modes of growth inhibition were determined. It was found that: (i) SL is maximal under conditions of amino acid starvation (-AA), the viable count decreasing by two orders of magnitude. (ii) SL is 4 times lower in the presence of chloramphenicol (-AA+CLP) and 6.5 times lower under +AA+CLP conditions. Changes in the sedimentation rate of DNA determined in alkaline sucrose gradient correlate with the differences in SL: under -AA conditions the sedimentation rate of DNA decreases whereas in the presence of CLP no decrease occurs. The results suggest that certain enzymatic processes take place under -AA conditions which lead to irreparable changes in DNA.
Authors:
Koukalova, B; Kuhrova, V [1] 
  1. Ceskoslovenska Akademie Ved, Brno. Biofysikalni Ustav
Publication Date:
May 01, 1980
Product Type:
Journal Article
Reference Number:
AIX-11-558637; EDB-81-002502
Resource Relation:
Journal Name: Int. J. Radiat. Biol.; (United Kingdom); Journal Volume: 37:5
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ESCHERICHIA COLI; LETHAL IRRADIATION; TRITIUM; BIOLOGICAL RADIATION EFFECTS; DECAY; DNA; GROWTH; LABELLED COMPOUNDS; MORTALITY; RADICALS; SUBLETHAL IRRADIATION; THYMIDINE; AZINES; BACTERIA; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL EFFECTS; HETEROCYCLIC COMPOUNDS; HYDROGEN ISOTOPES; IRRADIATION; ISOTOPES; LIGHT NUCLEI; MICROORGANISMS; NUCLEI; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PYRIMIDINES; RADIATION EFFECTS; RADIOISOTOPES; RIBOSIDES; YEARS LIVING RADIOISOTOPES; 560174* - Radiation Effects- Nuclide Kinetics & Toxicology- Microorganisms- (-1987)
OSTI ID:
6925726
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: IJRBA
Submitting Site:
INIS
Size:
Pages: 537-546
Announcement Date:
Dec 01, 1980

Journal Article:

Citation Formats

Koukalova, B, and Kuhrova, V. Damage to E. coli cells induced by tritium decay: secondary lethality under nongrowth conditions. United Kingdom: N. p., 1980. Web.
Koukalova, B, & Kuhrova, V. Damage to E. coli cells induced by tritium decay: secondary lethality under nongrowth conditions. United Kingdom.
Koukalova, B, and Kuhrova, V. 1980. "Damage to E. coli cells induced by tritium decay: secondary lethality under nongrowth conditions." United Kingdom.
@misc{etde_6925726,
title = {Damage to E. coli cells induced by tritium decay: secondary lethality under nongrowth conditions}
author = {Koukalova, B, and Kuhrova, V}
abstractNote = {Cells containing incorporated /sup 3/H-thymidine are damaged by its decay. It was found with E.coli TAU-bar cells that a small part of the damage is lethal whereas most of it is reparable and only potentially lethal. If cells are subjected to nongrowth conditions, the potentially lethal damage changes to lethal damage. This process is called secondary lethality (SL). The extent of SL and some changes in DNA under three different modes of growth inhibition were determined. It was found that: (i) SL is maximal under conditions of amino acid starvation (-AA), the viable count decreasing by two orders of magnitude. (ii) SL is 4 times lower in the presence of chloramphenicol (-AA+CLP) and 6.5 times lower under +AA+CLP conditions. Changes in the sedimentation rate of DNA determined in alkaline sucrose gradient correlate with the differences in SL: under -AA conditions the sedimentation rate of DNA decreases whereas in the presence of CLP no decrease occurs. The results suggest that certain enzymatic processes take place under -AA conditions which lead to irreparable changes in DNA.}
journal = {Int. J. Radiat. Biol.; (United Kingdom)}
volume = {37:5}
journal type = {AC}
place = {United Kingdom}
year = {1980}
month = {May}
}