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Preparation of sup 125 I-creatine phosphokinase-MM

Journal Article:

Abstract

{sup 125}I-creatine phosphokinase-MM ({sup 125}I-CPK-MM) was prepared by {sup 125}I-labelled Bolton-Hunter reagent (HPNS). Iodinating conditions of HPNS and its conjugation to protein were studied. {sup 125}I-CPK-MM with immune activity was obtained and used to establish the {sup 125}I-CPK-MM radioimmunoassay method by the General Hospital of PLA. {sup 125}I-CPK-MM in PBS-G solution containing 0.015 mol/l ethyl mercaptan at 4-10 deg C can be used for one month.
Authors:
Jingxian, Su; Jingmin, Ma [1] 
  1. Academia Sinica, Beijing, BJ (China). Inst. of Atomic Energy
Publication Date:
Sep 01, 1988
Product Type:
Journal Article
Reference Number:
AIX-21-042416; EDB-90-079394
Resource Relation:
Journal Name: Journal of Isotopes; (China); Journal Volume: 1:2
Subject:
38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY; PHOSPHOTRANSFERASES; LABELLING; IODINE 125; ISOENZYMES; LABELLED COMPOUNDS; PHOSPHOCREATINE; AMINO ACIDS; BETA DECAY RADIOISOTOPES; CARBOXYLIC ACIDS; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; ENZYMES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; PHOSPHORUS-GROUP TRANSFERASES; RADIOISOTOPES; TRANSFERASES; 400703* - Radiochemistry & Nuclear Chemistry- Radioisotope Production
OSTI ID:
6909560
Country of Origin:
China
Language:
Chinese
Other Identifying Numbers:
Journal ID: CODEN: XY942
Submitting Site:
INIS
Size:
Pages: 41-44
Announcement Date:

Journal Article:

Citation Formats

Jingxian, Su, and Jingmin, Ma. Preparation of sup 125 I-creatine phosphokinase-MM. China: N. p., 1988. Web.
Jingxian, Su, & Jingmin, Ma. Preparation of sup 125 I-creatine phosphokinase-MM. China.
Jingxian, Su, and Jingmin, Ma. 1988. "Preparation of sup 125 I-creatine phosphokinase-MM." China.
@misc{etde_6909560,
title = {Preparation of sup 125 I-creatine phosphokinase-MM}
author = {Jingxian, Su, and Jingmin, Ma}
abstractNote = {{sup 125}I-creatine phosphokinase-MM ({sup 125}I-CPK-MM) was prepared by {sup 125}I-labelled Bolton-Hunter reagent (HPNS). Iodinating conditions of HPNS and its conjugation to protein were studied. {sup 125}I-CPK-MM with immune activity was obtained and used to establish the {sup 125}I-CPK-MM radioimmunoassay method by the General Hospital of PLA. {sup 125}I-CPK-MM in PBS-G solution containing 0.015 mol/l ethyl mercaptan at 4-10 deg C can be used for one month.}
journal = {Journal of Isotopes; (China)}
volume = {1:2}
journal type = {AC}
place = {China}
year = {1988}
month = {Sep}
}