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Effects of interferon on cultured cells persistently infected with viruses

Thesis/Dissertation:

Abstract

The role of interferon (IFN) in viral persistence at the cellular level was investigated. Two types of persistent infections were chosen. The first type was cell lines which contained hepatitis B virus (HBV) DNA (PLC/PRF/5 and Hep 3B cells) uninfected control hepatoma cells, (Mahlavu, HA22T and Hep G2 cells) or simian virus 40 (SV40) DNA (C2, C6, C11 cells) and control uninfected (CV-1 cells). In the second type of infection Vero cells persistently infected with SSPE or Sendai virus were used. The aim of this work was to determine what effect IFN had in these infections in terms of its antiviral and antiproliferative effects; which of the two major IFN-induced pathways, E enzyme or protein kinase were induced; whether there were any differences in sensitivity to IFN between the DNA and RNA virus persistent infections. The anti-viral effect of IFN was examined by its ability to inhibit Sindbis virus replication using a radioimmunoassay system. The antiproliferative effect of IFN was determined by cell counting and /sup 3/H-thymidine incorporation. The activation of the ribonuclease F, determined by the inhibition of /sup 3/H-leucine incorporation after introduction of 2-5 actin into the cells, was variable, being activated in all cell lines with the  More>>
Authors:
Publication Date:
Jan 01, 1986
Product Type:
Thesis/Dissertation
Reference Number:
AIX-19-082877; EDB-88-157682
Resource Relation:
Other Information: Thesis (M.Sc.)
Subject:
60 APPLIED LIFE SCIENCES; DNA; INTERFERON; VIRAL DISEASES; CELL CULTURES; CELL PROLIFERATION; RNA; SENSITIVITY; ACTIN; ANIMAL CELLS; ATP; AUTORADIOGRAPHY; EXPERIMENTAL DATA; HEPATITIS; HEPATOMAS; IN VITRO; INCUBATION; INHIBITION; IODINE 125; LEUCINE; METHIONINE; PHOSPHORUS 32; RADIOIMMUNOASSAY; RNA-ASE; SULFUR 35; THYMIDINE; TRITIUM; VIRUSES; AMINO ACIDS; AZINES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBOXYLIC ACIDS; DATA; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM DISEASES; DISEASES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; ENZYMES; ESTERASES; EVEN-ODD NUCLEI; GROWTH FACTORS; HETEROCYCLIC COMPOUNDS; HYDROGEN ISOTOPES; HYDROLASES; IMMUNOASSAY; IMMUNOLOGY; INFECTIOUS DISEASES; INFORMATION; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; LIPOTROPIC FACTORS; LYMPHOKINES; MICROORGANISMS; MITOGENS; NEOPLASMS; NUCLEI; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; NUMERICAL DATA; ODD-EVEN NUCLEI; ODD-ODD NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PARASITES; PHOSPHODIESTERASES; PHOSPHORUS ISOTOPES; PROTEINS; PYRIMIDINES; RADIOASSAY; RADIOIMMUNOLOGY; RADIOISOTOPES; RIBOSIDES; SULFUR ISOTOPES; TRACER TECHNIQUES; YEARS LIVING RADIOISOTOPES; 553003* - Agriculture & Food Technology- Pest & Disease Control- (1987-)
OSTI ID:
6908371
Research Organizations:
University of the Witwatersrand, Johannesburg (South Africa)
Country of Origin:
South Africa
Language:
English
Availability:
Registrar, University of the Witwatersrand, 1 Jan Smuts Avenue, Johannesburg, 2001, South Africa.
Submitting Site:
INIS
Size:
Pages: 196
Announcement Date:

Thesis/Dissertation:

Citation Formats

Crespi, M. Effects of interferon on cultured cells persistently infected with viruses. South Africa: N. p., 1986. Web.
Crespi, M. Effects of interferon on cultured cells persistently infected with viruses. South Africa.
Crespi, M. 1986. "Effects of interferon on cultured cells persistently infected with viruses." South Africa.
@misc{etde_6908371,
title = {Effects of interferon on cultured cells persistently infected with viruses}
author = {Crespi, M}
abstractNote = {The role of interferon (IFN) in viral persistence at the cellular level was investigated. Two types of persistent infections were chosen. The first type was cell lines which contained hepatitis B virus (HBV) DNA (PLC/PRF/5 and Hep 3B cells) uninfected control hepatoma cells, (Mahlavu, HA22T and Hep G2 cells) or simian virus 40 (SV40) DNA (C2, C6, C11 cells) and control uninfected (CV-1 cells). In the second type of infection Vero cells persistently infected with SSPE or Sendai virus were used. The aim of this work was to determine what effect IFN had in these infections in terms of its antiviral and antiproliferative effects; which of the two major IFN-induced pathways, E enzyme or protein kinase were induced; whether there were any differences in sensitivity to IFN between the DNA and RNA virus persistent infections. The anti-viral effect of IFN was examined by its ability to inhibit Sindbis virus replication using a radioimmunoassay system. The antiproliferative effect of IFN was determined by cell counting and /sup 3/H-thymidine incorporation. The activation of the ribonuclease F, determined by the inhibition of /sup 3/H-leucine incorporation after introduction of 2-5 actin into the cells, was variable, being activated in all cell lines with the exception of the PLC/PRF/5, Hep 3B and Hep G2 cells. Major differences between the two DNA persistent infections and the two RNA persistent infections were found. No correlation was found between the presence of HBV or SV40 persistent infections and the sensitivity of the cell lines to IFN. Both the SSPE and Sendai virus persistent infections were resistant to the antiviral and antiproliferative effect of IFN.}
place = {South Africa}
year = {1986}
month = {Jan}
}