You need JavaScript to view this

Iodo-gen-catalysed iodination for identification of surface-exposed outer membrane proteins of Escherichia coli K12

Journal Article:

Abstract

Surface proteins of Escherichia coli K12 were identified by radiolabelling using 1,3,4,6 - tatrachloro, 3-alpha, 6-alpha - diphenylgycoluryl (Iodo-Gen) and /sup 131/I. Labelled proteins were localized in the outer membrane of the cells. Using this technique it has been possible to observe technique it has been possible to observe that the eletrophoretic pattern of surface proteins changes according to the growth phases in culture. Radiolabelling of E.coli cells inculbated at 42/sup 0/C showed that the syntheses of two surface proteins were temperature-inducible. At least one such protein may be involved in the process of cell division in E.coli K12.
Publication Date:
Dec 01, 1987
Product Type:
Journal Article
Reference Number:
AIX-19-083140; EDB-88-157700
Resource Relation:
Journal Name: Rev. Bras. Genet.; (Brazil); Journal Volume: 10:4
Subject:
60 APPLIED LIFE SCIENCES; CELL MEMBRANES; CELL DIVISION; PROTEINS; ESCHERICHIA COLI; BIOSYNTHESIS; IODINE 131; LABELLING; TEMPERATURE DEPENDENCE; BACTERIA; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CELL CONSTITUENTS; DAYS LIVING RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPES; MEMBRANES; MICROORGANISMS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; RADIOISOTOPES; SYNTHESIS; 553006* - Agriculture & Food Technology- Other Agricultural Applications- (1987-)
OSTI ID:
6908337
Research Organizations:
Pernambuco Univ., Recife (Brazil). Dept. de Biofisica e Radiobiologia; Rio de Janeiro Univ. (Brazil). Inst. de Biofisica
Country of Origin:
Brazil
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: RBGED
Submitting Site:
INIS
Size:
Pages: 625-634
Announcement Date:

Journal Article:

Citation Formats

Ferreira, L C.S., and Almeida, D.F. de. Iodo-gen-catalysed iodination for identification of surface-exposed outer membrane proteins of Escherichia coli K12. Brazil: N. p., 1987. Web.
Ferreira, L C.S., & Almeida, D.F. de. Iodo-gen-catalysed iodination for identification of surface-exposed outer membrane proteins of Escherichia coli K12. Brazil.
Ferreira, L C.S., and Almeida, D.F. de. 1987. "Iodo-gen-catalysed iodination for identification of surface-exposed outer membrane proteins of Escherichia coli K12." Brazil.
@misc{etde_6908337,
title = {Iodo-gen-catalysed iodination for identification of surface-exposed outer membrane proteins of Escherichia coli K12}
author = {Ferreira, L C.S., and Almeida, D.F. de}
abstractNote = {Surface proteins of Escherichia coli K12 were identified by radiolabelling using 1,3,4,6 - tatrachloro, 3-alpha, 6-alpha - diphenylgycoluryl (Iodo-Gen) and /sup 131/I. Labelled proteins were localized in the outer membrane of the cells. Using this technique it has been possible to observe technique it has been possible to observe that the eletrophoretic pattern of surface proteins changes according to the growth phases in culture. Radiolabelling of E.coli cells inculbated at 42/sup 0/C showed that the syntheses of two surface proteins were temperature-inducible. At least one such protein may be involved in the process of cell division in E.coli K12.}
journal = {Rev. Bras. Genet.; (Brazil)}
volume = {10:4}
journal type = {AC}
place = {Brazil}
year = {1987}
month = {Dec}
}