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Radiation induced formation of giant cells (Saccharomyces uvarum). Pt. 1

Journal Article:

Abstract

X-irradiated yeast cells (Saccharomyces uvarum) grown in liquid media stop mitosis and form giant cells. Chitin ring formation, being a prerequisite for cell separation, was studied by fluorescence microscopy using Calcofluor White, a chitin specific dye. Experiments with inhibitors of DNA synthesis (hydroxyurea) and chitin synthesis (polyoxin D) demonstrate chitin ring formation to be dependent on DNA synthesis, whereas bud formation is independent of DNA synthesis and chitin ring formation respectively. Basing on these results the formation of X-ray induced giant cells implies one DNA replication which in turn induces the formation of only one chitin ring between mother cell and giant bud. Obviously no septum can be formed. Thus cell separation does not occur, but the bud already formed, produces another bud demonstrating that bud formation itself is independent of DNA synthesis.
Publication Date:
Feb 01, 1984
Product Type:
Journal Article
Reference Number:
EDB-84-095397
Resource Relation:
Journal Name: Radiat. Environ. Biophys.; (Germany, Federal Republic of); Journal Volume: 23:1
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CELL PROLIFERATION; BIOLOGICAL RADIATION EFFECTS; SACCHAROMYCES; BIOSYNTHESIS; CELL CYCLE; CHITIN; DNA; DNA REPLICATION; EXTERNAL IRRADIATION; INHIBITION; SURVIVAL CURVES; X RADIATION; AMINES; BIOLOGICAL EFFECTS; CARBOHYDRATES; ELECTROMAGNETIC RADIATION; FUNGI; IONIZING RADIATIONS; IRRADIATION; MICROORGANISMS; MUCOPOLYSACCHARIDES; NUCLEIC ACID REPLICATION; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PLANTS; POLYSACCHARIDES; RADIATION EFFECTS; RADIATIONS; SACCHARIDES; SYNTHESIS; YEASTS; 560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987); 560202 - Thermal Effects- Microorganisms- (1987)
OSTI ID:
6905335
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: REBPA
Submitting Site:
HEDB
Size:
Pages: 19-30
Announcement Date:

Journal Article:

Citation Formats

Baumstark-Khan, C, Schnitzler, L, and Rink, H. Radiation induced formation of giant cells (Saccharomyces uvarum). Pt. 1. Germany: N. p., 1984. Web.
Baumstark-Khan, C, Schnitzler, L, & Rink, H. Radiation induced formation of giant cells (Saccharomyces uvarum). Pt. 1. Germany.
Baumstark-Khan, C, Schnitzler, L, and Rink, H. 1984. "Radiation induced formation of giant cells (Saccharomyces uvarum). Pt. 1." Germany.
@misc{etde_6905335,
title = {Radiation induced formation of giant cells (Saccharomyces uvarum). Pt. 1}
author = {Baumstark-Khan, C, Schnitzler, L, and Rink, H}
abstractNote = {X-irradiated yeast cells (Saccharomyces uvarum) grown in liquid media stop mitosis and form giant cells. Chitin ring formation, being a prerequisite for cell separation, was studied by fluorescence microscopy using Calcofluor White, a chitin specific dye. Experiments with inhibitors of DNA synthesis (hydroxyurea) and chitin synthesis (polyoxin D) demonstrate chitin ring formation to be dependent on DNA synthesis, whereas bud formation is independent of DNA synthesis and chitin ring formation respectively. Basing on these results the formation of X-ray induced giant cells implies one DNA replication which in turn induces the formation of only one chitin ring between mother cell and giant bud. Obviously no septum can be formed. Thus cell separation does not occur, but the bud already formed, produces another bud demonstrating that bud formation itself is independent of DNA synthesis.}
journal = {Radiat. Environ. Biophys.; (Germany, Federal Republic of)}
volume = {23:1}
journal type = {AC}
place = {Germany}
year = {1984}
month = {Feb}
}