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Ethanol production by Clostridium thermocellum grown on hydrothermally and organosolv-pretreated lignocellulosic materials

Journal Article:

Abstract

Two strains of the thermophilic anaerobe Clostridium thermocellum, the wild type NCIB 10682 and its ethanol-hyperproductive mutant 647, were tested for their ability to grow on natural lignocellulosic materials (poplar wood, wheat straw) which had been pretreated by either hydrothermolysis or an organosolv process. For both materials and both strains, the dependencies of substrate accessibility on the pretreatment temperature were established in terms of cellulose hydrolysis and of product formation. In addition to the non-pH-controlled shake flask assays, in vitro experiments with cell-free culture supernatant and in vivo cellulolyses under pH regulation in a laboratory fermenter indicated that lignocellulosics pretreated at approx. 230/sup 0/C were degraded efficiently by the Clostridium strains investigated.
Publication Date:
Dec 01, 1988
Product Type:
Journal Article
Reference Number:
DE-88-016484; EDB-89-012601
Resource Relation:
Journal Name: Appl. Microbiol. Biotechnol.; (Germany, Federal Republic of); Journal Volume: 29:6
Subject:
09 BIOMASS FUELS; CELLULOSE; ENZYMATIC HYDROLYSIS; CLOSTRIDIUM; CELL CULTURES; ETHANOL; PRODUCTION; BENCH-SCALE EXPERIMENTS; CELLULASE; CULTURE MEDIA; LIGNIN; LIQUID COLUMN CHROMATOGRAPHY; POPLARS; STRAINS; WOOD; ALCOHOLS; BACTERIA; CARBOHYDRATES; CHEMICAL REACTIONS; CHROMATOGRAPHY; DECOMPOSITION; ENZYMES; GLYCOSYL HYDROLASES; HYDROLASES; HYDROLYSIS; HYDROXY COMPOUNDS; LYSIS; MICROORGANISMS; O-GLYCOSYL HYDROLASES; ORGANIC COMPOUNDS; PLANTS; POLYSACCHARIDES; SACCHARIDES; SEPARATION PROCESSES; SOLVOLYSIS; TREES; 090222* - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989); 140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989)
OSTI ID:
6898427
Research Organizations:
Innsbruck Univ. (Austria). Inst. fuer Radiochemie und Angewandte Physikalische Chemie; Institut Pasteur, 75 - Paris (France). Unite de Physiologie Cellulaire
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: AMBID
Submitting Site:
DE
Size:
Pages: 528-535
Announcement Date:

Journal Article:

Citation Formats

Hoermeyer, H F, Bonn, G, Bobleter, O, Tailliez, P, Millet, J, Girard, H, and Aubert, J P. Ethanol production by Clostridium thermocellum grown on hydrothermally and organosolv-pretreated lignocellulosic materials. Germany: N. p., 1988. Web.
Hoermeyer, H F, Bonn, G, Bobleter, O, Tailliez, P, Millet, J, Girard, H, & Aubert, J P. Ethanol production by Clostridium thermocellum grown on hydrothermally and organosolv-pretreated lignocellulosic materials. Germany.
Hoermeyer, H F, Bonn, G, Bobleter, O, Tailliez, P, Millet, J, Girard, H, and Aubert, J P. 1988. "Ethanol production by Clostridium thermocellum grown on hydrothermally and organosolv-pretreated lignocellulosic materials." Germany.
@misc{etde_6898427,
title = {Ethanol production by Clostridium thermocellum grown on hydrothermally and organosolv-pretreated lignocellulosic materials}
author = {Hoermeyer, H F, Bonn, G, Bobleter, O, Tailliez, P, Millet, J, Girard, H, and Aubert, J P}
abstractNote = {Two strains of the thermophilic anaerobe Clostridium thermocellum, the wild type NCIB 10682 and its ethanol-hyperproductive mutant 647, were tested for their ability to grow on natural lignocellulosic materials (poplar wood, wheat straw) which had been pretreated by either hydrothermolysis or an organosolv process. For both materials and both strains, the dependencies of substrate accessibility on the pretreatment temperature were established in terms of cellulose hydrolysis and of product formation. In addition to the non-pH-controlled shake flask assays, in vitro experiments with cell-free culture supernatant and in vivo cellulolyses under pH regulation in a laboratory fermenter indicated that lignocellulosics pretreated at approx. 230/sup 0/C were degraded efficiently by the Clostridium strains investigated.}
journal = {Appl. Microbiol. Biotechnol.; (Germany, Federal Republic of)}
volume = {29:6}
journal type = {AC}
place = {Germany}
year = {1988}
month = {Dec}
}