Abstract
A nearly full-length cDNA encoding the human vitamin D binding protein (hDBP), also known as Gc-globulin, was isolated from a human liver cDNA expression library. This 1.73 kb cDNA was digested with EcoRI and the 5{prime}, 140 bp fragment of the cDNA, subcloned into plasmid SP65 (phDBP140), was used as probe. MspI identifies a two allele polymorphism with either a band at 12 kb or a band at 5 kb. The frequency was estimated from a study of 24 unrelated North American Caucasians. The hDBP gene has been localized to chromosome 4 using a cDNA probe and a panel of rodent X human somatic cell hybrids. It was sublocalized to 4q11-q13 by in situ hybridization. Co-dominant autosomal segregation of the polymorphic alleles has been observed in two informative families (20 individuals). With overexposure of the autoradiograph two faint variant bands are seen at 17 kb and 13.5 kb which appear to cosegregate with the 12 kb band and the 5 kb band respectively.
Citation Formats
Ray, K, and Cooke, N E.
A high frequency MspI RFLP at the human vitamin D binding protein (hDBP) locus.
United Kingdom: N. p.,
1988.
Web.
doi:10.1093/nar/16.14.7211.
Ray, K, & Cooke, N E.
A high frequency MspI RFLP at the human vitamin D binding protein (hDBP) locus.
United Kingdom.
https://doi.org/10.1093/nar/16.14.7211
Ray, K, and Cooke, N E.
1988.
"A high frequency MspI RFLP at the human vitamin D binding protein (hDBP) locus."
United Kingdom.
https://doi.org/10.1093/nar/16.14.7211.
@misc{etde_6897709,
title = {A high frequency MspI RFLP at the human vitamin D binding protein (hDBP) locus}
author = {Ray, K, and Cooke, N E}
abstractNote = {A nearly full-length cDNA encoding the human vitamin D binding protein (hDBP), also known as Gc-globulin, was isolated from a human liver cDNA expression library. This 1.73 kb cDNA was digested with EcoRI and the 5{prime}, 140 bp fragment of the cDNA, subcloned into plasmid SP65 (phDBP140), was used as probe. MspI identifies a two allele polymorphism with either a band at 12 kb or a band at 5 kb. The frequency was estimated from a study of 24 unrelated North American Caucasians. The hDBP gene has been localized to chromosome 4 using a cDNA probe and a panel of rodent X human somatic cell hybrids. It was sublocalized to 4q11-q13 by in situ hybridization. Co-dominant autosomal segregation of the polymorphic alleles has been observed in two informative families (20 individuals). With overexposure of the autoradiograph two faint variant bands are seen at 17 kb and 13.5 kb which appear to cosegregate with the 12 kb band and the 5 kb band respectively.}
doi = {10.1093/nar/16.14.7211}
journal = []
volume = {16:14B}
journal type = {AC}
place = {United Kingdom}
year = {1988}
month = {Jul}
}
title = {A high frequency MspI RFLP at the human vitamin D binding protein (hDBP) locus}
author = {Ray, K, and Cooke, N E}
abstractNote = {A nearly full-length cDNA encoding the human vitamin D binding protein (hDBP), also known as Gc-globulin, was isolated from a human liver cDNA expression library. This 1.73 kb cDNA was digested with EcoRI and the 5{prime}, 140 bp fragment of the cDNA, subcloned into plasmid SP65 (phDBP140), was used as probe. MspI identifies a two allele polymorphism with either a band at 12 kb or a band at 5 kb. The frequency was estimated from a study of 24 unrelated North American Caucasians. The hDBP gene has been localized to chromosome 4 using a cDNA probe and a panel of rodent X human somatic cell hybrids. It was sublocalized to 4q11-q13 by in situ hybridization. Co-dominant autosomal segregation of the polymorphic alleles has been observed in two informative families (20 individuals). With overexposure of the autoradiograph two faint variant bands are seen at 17 kb and 13.5 kb which appear to cosegregate with the 12 kb band and the 5 kb band respectively.}
doi = {10.1093/nar/16.14.7211}
journal = []
volume = {16:14B}
journal type = {AC}
place = {United Kingdom}
year = {1988}
month = {Jul}
}