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Purified human somatomedin A and rat multiplication stimulating activity

Abstract

Specific receptors for MSA and/or somatomedin A could be demonstrated in intact cells or membranes from chick embryo fibroblasts, human fibroblasts, human placenta, rat liver, and the BRL 3A2 cell line, a subclone of the line that produces MSA. Unlabeled MSA and somatomedin A inhibited the binding of /sup 125/I-labeled MSA and /sup 125/I-labeled somatomedin A to each of these receptors with comparable potency. In chick embryo fibroblasts, human fibroblasts, and human placental membranes, the binding of both radioactive ligands also was inhibited by insulin, consistent with the interpretation that /sup 125/I-labeled MSA and /sup 125/I-labeled somatomedin A were binding to the same receptor. By contrast, in the BRL 3A2 cell line, insulin inhibited the binding of /sup 125/I-labeled somatomedin A, but not the binding of /sup 125/I-labeled MSA, suggesting that the two labeled peptides were binding to different receptors in this cell line. Moreover, /sup 125/I-labeled MSA, but not /sup 125/I-labeled somatomedin A, bound specifically to rat liver plasma membranes. These results indicate that human somatomedin A and rat MSA are closely related, but not identical, peptides.
Publication Date:
Jan 01, 1978
Product Type:
Journal Article
Reference Number:
AIX-09-386200; EDB-78-098583
Resource Relation:
Journal Name: Eur. J. Biochem.; (Germany, Federal Republic of); Journal Volume: 82:1
Subject:
59 BASIC BIOLOGICAL SCIENCES; METABOLISM; BIOCHEMICAL REACTION KINETICS; CHICKENS; DNA; DOSE RATES; DOSE-RESPONSE RELATIONSHIPS; FIBROBLASTS; IODINE 125; LABELLED COMPOUNDS; MAN; MULTIPLICATION FACTORS; ORGANS; PEPTIDES; RADIOACTIVITY; RATS; STIMULI; SYNTHESIS; TRACER TECHNIQUES; ANIMAL CELLS; ANIMALS; BETA DECAY RADIOISOTOPES; BIRDS; BODY; CONNECTIVE TISSUE CELLS; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; FOWL; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; MAMMALS; NUCLEI; NUCLEIC ACIDS; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PRIMATES; PROTEINS; RADIOISOTOPES; REACTION KINETICS; RODENTS; SOMATIC CELLS; VERTEBRATES; 550501* - Metabolism- Tracer Techniques
OSTI ID:
6881373
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: EJBCA
Submitting Site:
INIS
Size:
Pages: 5-12
Announcement Date:
Jul 01, 1978

Citation Formats

Rechler, M M, Fryklund, L, Nissley, S P, Hall, K, Podskalny, J M, Skottner, A, Moses, A C, Kabi AB, Stockholm, and National Inst. of Arthritis, Metabolism and Digestive Diseases, Bethesda, Md. (USA). Diabetes Branch). Purified human somatomedin A and rat multiplication stimulating activity. Germany: N. p., 1978. Web.
Rechler, M M, Fryklund, L, Nissley, S P, Hall, K, Podskalny, J M, Skottner, A, Moses, A C, Kabi AB, Stockholm, & National Inst. of Arthritis, Metabolism and Digestive Diseases, Bethesda, Md. (USA). Diabetes Branch). Purified human somatomedin A and rat multiplication stimulating activity. Germany.
Rechler, M M, Fryklund, L, Nissley, S P, Hall, K, Podskalny, J M, Skottner, A, Moses, A C, Kabi AB, Stockholm, and National Inst. of Arthritis, Metabolism and Digestive Diseases, Bethesda, Md. (USA). Diabetes Branch). 1978. "Purified human somatomedin A and rat multiplication stimulating activity." Germany.
@misc{etde_6881373,
title = {Purified human somatomedin A and rat multiplication stimulating activity}
author = {Rechler, M M, Fryklund, L, Nissley, S P, Hall, K, Podskalny, J M, Skottner, A, Moses, A C, Kabi AB, Stockholm, and National Inst. of Arthritis, Metabolism and Digestive Diseases, Bethesda, Md. (USA). Diabetes Branch)}
abstractNote = {Specific receptors for MSA and/or somatomedin A could be demonstrated in intact cells or membranes from chick embryo fibroblasts, human fibroblasts, human placenta, rat liver, and the BRL 3A2 cell line, a subclone of the line that produces MSA. Unlabeled MSA and somatomedin A inhibited the binding of /sup 125/I-labeled MSA and /sup 125/I-labeled somatomedin A to each of these receptors with comparable potency. In chick embryo fibroblasts, human fibroblasts, and human placental membranes, the binding of both radioactive ligands also was inhibited by insulin, consistent with the interpretation that /sup 125/I-labeled MSA and /sup 125/I-labeled somatomedin A were binding to the same receptor. By contrast, in the BRL 3A2 cell line, insulin inhibited the binding of /sup 125/I-labeled somatomedin A, but not the binding of /sup 125/I-labeled MSA, suggesting that the two labeled peptides were binding to different receptors in this cell line. Moreover, /sup 125/I-labeled MSA, but not /sup 125/I-labeled somatomedin A, bound specifically to rat liver plasma membranes. These results indicate that human somatomedin A and rat MSA are closely related, but not identical, peptides.}
journal = []
volume = {82:1}
journal type = {AC}
place = {Germany}
year = {1978}
month = {Jan}
}