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Study on the kinetics of high-energy phosphates in myocardium by phosphorous nuclear magnetic resonance

Abstract

Effect of artificial blood, FC 43 (Perfluorochemicals) on the kinetics of high-energy phosphate in the myocardium was evaluated by /sup 31/P-NMR which permits a continuous and non-invasive assessment of in vivo phosphorus compounds. Cardiac perfusion was carried out on a excised rat heart with a Krebs-Henseleit modified solution and FC 43 alternately. Under the normal condition, ischemic condition, and at second perfusion amounts of intramyocardial creatine phosphoric acid, ATP, and inorganic phosphorus were determined by /sup 31/P-NMR. Coronary flow was simultaneously estimated. The ischemic state due to interruption of perfusion resulted in a decrease in creatine phosphoric acid, which was associated with an increase in inorganic phosphorus and intracellular acidosis. No change of ATP amount was observed under ischemic state. With resumption of perfusion, the levels of creatine phosphoric acid and inorganic phosphorus rapidly returned to the normal. In the group of FC 43, coronary flow was 2.68 ml/min/g of the heart weight, about 1/2 of that of the Krebs-Henseleit group (5.68 ml/min/g of the heart weight). In controls, there was no difference between the two groups concerning creatine phosphoric acid level and recovery of creatine phospohric acid level after ischemia. These results showed that FC 43 supplies sufficient oxygen,  More>>
Authors:
Kusuoka, Hideo; Tsuneoka, Yutaka; Inoue, Michitoshi; Abe, Hiroshi; [1]  Watari, Hiroshi
  1. Osaka Univ. (Japan). Faculty of Medicine
Publication Date:
Dec 01, 1982
Product Type:
Journal Article
Reference Number:
AIX-15-052527; EDB-84-150542
Resource Relation:
Journal Name: NMR Igaku; (Japan); Journal Volume: 2:1
Subject:
59 BASIC BIOLOGICAL SCIENCES; MYOCARDIUM; NMR SPECTRA; PHOSPHATES; METABOLISM; PHOSPHORUS 31; NUCLEAR MAGNETIC RESONANCE; PHOSPHORYLATION; ATP; BIOLOGICAL PATHWAYS; BLOOD FLOW; CORONARIES; PERFUSED ORGANS; PHOSPHOCREATINE; QUANTITY RATIO; RATS; AMINO ACIDS; ANIMALS; ARTERIES; BLOOD VESSELS; BODY; CARBOXYLIC ACIDS; CARDIOVASCULAR SYSTEM; CHEMICAL REACTIONS; HEART; ISOTOPES; LIGHT NUCLEI; MAGNETIC RESONANCE; MAMMALS; MUSCLES; NUCLEI; NUCLEOTIDES; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; ORGANS; OXYGEN COMPOUNDS; PHOSPHORUS COMPOUNDS; PHOSPHORUS ISOTOPES; RESONANCE; RODENTS; SPECTRA; STABLE ISOTOPES; VERTEBRATES; 550501* - Metabolism- Tracer Techniques
OSTI ID:
6868704
Country of Origin:
Japan
Language:
Japanese
Other Identifying Numbers:
Journal ID: CODEN: NMRIE
Submitting Site:
HEDB
Size:
Pages: 41-48
Announcement Date:
Aug 01, 1984

Citation Formats

Kusuoka, Hideo, Tsuneoka, Yutaka, Inoue, Michitoshi, Abe, Hiroshi, and Watari, Hiroshi. Study on the kinetics of high-energy phosphates in myocardium by phosphorous nuclear magnetic resonance. Japan: N. p., 1982. Web.
Kusuoka, Hideo, Tsuneoka, Yutaka, Inoue, Michitoshi, Abe, Hiroshi, & Watari, Hiroshi. Study on the kinetics of high-energy phosphates in myocardium by phosphorous nuclear magnetic resonance. Japan.
Kusuoka, Hideo, Tsuneoka, Yutaka, Inoue, Michitoshi, Abe, Hiroshi, and Watari, Hiroshi. 1982. "Study on the kinetics of high-energy phosphates in myocardium by phosphorous nuclear magnetic resonance." Japan.
@misc{etde_6868704,
title = {Study on the kinetics of high-energy phosphates in myocardium by phosphorous nuclear magnetic resonance}
author = {Kusuoka, Hideo, Tsuneoka, Yutaka, Inoue, Michitoshi, Abe, Hiroshi, and Watari, Hiroshi}
abstractNote = {Effect of artificial blood, FC 43 (Perfluorochemicals) on the kinetics of high-energy phosphate in the myocardium was evaluated by /sup 31/P-NMR which permits a continuous and non-invasive assessment of in vivo phosphorus compounds. Cardiac perfusion was carried out on a excised rat heart with a Krebs-Henseleit modified solution and FC 43 alternately. Under the normal condition, ischemic condition, and at second perfusion amounts of intramyocardial creatine phosphoric acid, ATP, and inorganic phosphorus were determined by /sup 31/P-NMR. Coronary flow was simultaneously estimated. The ischemic state due to interruption of perfusion resulted in a decrease in creatine phosphoric acid, which was associated with an increase in inorganic phosphorus and intracellular acidosis. No change of ATP amount was observed under ischemic state. With resumption of perfusion, the levels of creatine phosphoric acid and inorganic phosphorus rapidly returned to the normal. In the group of FC 43, coronary flow was 2.68 ml/min/g of the heart weight, about 1/2 of that of the Krebs-Henseleit group (5.68 ml/min/g of the heart weight). In controls, there was no difference between the two groups concerning creatine phosphoric acid level and recovery of creatine phospohric acid level after ischemia. These results showed that FC 43 supplies sufficient oxygen, and has no effect on the kinetics of energy in the myocardium.}
journal = []
volume = {2:1}
journal type = {AC}
place = {Japan}
year = {1982}
month = {Dec}
}