Abstract
Effect of artificial blood, FC 43 (Perfluorochemicals) on the kinetics of high-energy phosphate in the myocardium was evaluated by /sup 31/P-NMR which permits a continuous and non-invasive assessment of in vivo phosphorus compounds. Cardiac perfusion was carried out on a excised rat heart with a Krebs-Henseleit modified solution and FC 43 alternately. Under the normal condition, ischemic condition, and at second perfusion amounts of intramyocardial creatine phosphoric acid, ATP, and inorganic phosphorus were determined by /sup 31/P-NMR. Coronary flow was simultaneously estimated. The ischemic state due to interruption of perfusion resulted in a decrease in creatine phosphoric acid, which was associated with an increase in inorganic phosphorus and intracellular acidosis. No change of ATP amount was observed under ischemic state. With resumption of perfusion, the levels of creatine phosphoric acid and inorganic phosphorus rapidly returned to the normal. In the group of FC 43, coronary flow was 2.68 ml/min/g of the heart weight, about 1/2 of that of the Krebs-Henseleit group (5.68 ml/min/g of the heart weight). In controls, there was no difference between the two groups concerning creatine phosphoric acid level and recovery of creatine phospohric acid level after ischemia. These results showed that FC 43 supplies sufficient oxygen,
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Kusuoka, Hideo;
Tsuneoka, Yutaka;
Inoue, Michitoshi;
Abe, Hiroshi;
[1]
Watari, Hiroshi
- Osaka Univ. (Japan). Faculty of Medicine
Citation Formats
Kusuoka, Hideo, Tsuneoka, Yutaka, Inoue, Michitoshi, Abe, Hiroshi, and Watari, Hiroshi.
Study on the kinetics of high-energy phosphates in myocardium by phosphorous nuclear magnetic resonance.
Japan: N. p.,
1982.
Web.
Kusuoka, Hideo, Tsuneoka, Yutaka, Inoue, Michitoshi, Abe, Hiroshi, & Watari, Hiroshi.
Study on the kinetics of high-energy phosphates in myocardium by phosphorous nuclear magnetic resonance.
Japan.
Kusuoka, Hideo, Tsuneoka, Yutaka, Inoue, Michitoshi, Abe, Hiroshi, and Watari, Hiroshi.
1982.
"Study on the kinetics of high-energy phosphates in myocardium by phosphorous nuclear magnetic resonance."
Japan.
@misc{etde_6868704,
title = {Study on the kinetics of high-energy phosphates in myocardium by phosphorous nuclear magnetic resonance}
author = {Kusuoka, Hideo, Tsuneoka, Yutaka, Inoue, Michitoshi, Abe, Hiroshi, and Watari, Hiroshi}
abstractNote = {Effect of artificial blood, FC 43 (Perfluorochemicals) on the kinetics of high-energy phosphate in the myocardium was evaluated by /sup 31/P-NMR which permits a continuous and non-invasive assessment of in vivo phosphorus compounds. Cardiac perfusion was carried out on a excised rat heart with a Krebs-Henseleit modified solution and FC 43 alternately. Under the normal condition, ischemic condition, and at second perfusion amounts of intramyocardial creatine phosphoric acid, ATP, and inorganic phosphorus were determined by /sup 31/P-NMR. Coronary flow was simultaneously estimated. The ischemic state due to interruption of perfusion resulted in a decrease in creatine phosphoric acid, which was associated with an increase in inorganic phosphorus and intracellular acidosis. No change of ATP amount was observed under ischemic state. With resumption of perfusion, the levels of creatine phosphoric acid and inorganic phosphorus rapidly returned to the normal. In the group of FC 43, coronary flow was 2.68 ml/min/g of the heart weight, about 1/2 of that of the Krebs-Henseleit group (5.68 ml/min/g of the heart weight). In controls, there was no difference between the two groups concerning creatine phosphoric acid level and recovery of creatine phospohric acid level after ischemia. These results showed that FC 43 supplies sufficient oxygen, and has no effect on the kinetics of energy in the myocardium.}
journal = []
volume = {2:1}
journal type = {AC}
place = {Japan}
year = {1982}
month = {Dec}
}
title = {Study on the kinetics of high-energy phosphates in myocardium by phosphorous nuclear magnetic resonance}
author = {Kusuoka, Hideo, Tsuneoka, Yutaka, Inoue, Michitoshi, Abe, Hiroshi, and Watari, Hiroshi}
abstractNote = {Effect of artificial blood, FC 43 (Perfluorochemicals) on the kinetics of high-energy phosphate in the myocardium was evaluated by /sup 31/P-NMR which permits a continuous and non-invasive assessment of in vivo phosphorus compounds. Cardiac perfusion was carried out on a excised rat heart with a Krebs-Henseleit modified solution and FC 43 alternately. Under the normal condition, ischemic condition, and at second perfusion amounts of intramyocardial creatine phosphoric acid, ATP, and inorganic phosphorus were determined by /sup 31/P-NMR. Coronary flow was simultaneously estimated. The ischemic state due to interruption of perfusion resulted in a decrease in creatine phosphoric acid, which was associated with an increase in inorganic phosphorus and intracellular acidosis. No change of ATP amount was observed under ischemic state. With resumption of perfusion, the levels of creatine phosphoric acid and inorganic phosphorus rapidly returned to the normal. In the group of FC 43, coronary flow was 2.68 ml/min/g of the heart weight, about 1/2 of that of the Krebs-Henseleit group (5.68 ml/min/g of the heart weight). In controls, there was no difference between the two groups concerning creatine phosphoric acid level and recovery of creatine phospohric acid level after ischemia. These results showed that FC 43 supplies sufficient oxygen, and has no effect on the kinetics of energy in the myocardium.}
journal = []
volume = {2:1}
journal type = {AC}
place = {Japan}
year = {1982}
month = {Dec}
}