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Molecular radiobiology of nucleic acids

Thesis/Dissertation:

Abstract

In addition to radiolytic adenine release, radiolysis of adenosine 5'-monophosphate, in the absence of oxygen, can result in the formation of 8-hydroxyadenosine 5'-monophosphate and both the (R)- and (S)-epimer of 8,5'-cycloadenosine 5'-monophosphate. The mononucleoside derivatives of these modified nucleotides were also observed in irradiated solutions of adenosine and in the enzyme hydrolysates of irradiated solutions of polyadenylic acid (poly A) using high-performance liquid chromatography (HPLC). In an effort to detect 8,5'-cyclo(deoxy) adenosine formation in irradiated nucleic acids, polyclonal antiserum were raised with specificity to the 8,5'-cycloadenosine 5'-monophosphate moiety and used in an enzyme-linked immunosorbent assay (ELISA). The 8,5'-cyclo(deoxy)adenosine moiety could be detected in nitrous oxide-saturated aqueous solutions containing unhydrolyzed poly A at 10 Gy and DNA at 200 Gy using the colorimetric ELISA. Correlation of product yield measured by ELISA with HPLC analysis of irradiated, enzyme-hydrolyzed solutions of poly A revealed that the ELISA was precisely reflecting changes in the combined yield of (R)- and (S)-8,5'-cycloadenosine.
Authors:
Publication Date:
Jan 01, 1987
Product Type:
Thesis/Dissertation
Reference Number:
EDB-88-150903
Resource Relation:
Other Information: Thesis (Ph. D.)
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ADENYLIC ACID; CHEMICAL RADIATION EFFECTS; DNA; NUCLEIC ACIDS; RADIOBIOLOGY; ADENOSINE; ENZYME IMMUNOASSAY; LIQUID COLUMN CHROMATOGRAPHY; MOLECULAR BIOLOGY; NUCLEOTIDES; BIOLOGY; CHEMISTRY; CHROMATOGRAPHY; IMMUNOASSAY; NUCLEOSIDES; ORGANIC COMPOUNDS; RADIATION CHEMISTRY; RADIATION EFFECTS; RIBOSIDES; SEPARATION PROCESSES; 560120* - Radiation Effects on Biochemicals, Cells, & Tissue Culture
OSTI ID:
6856619
Research Organizations:
Alberta Univ., Edmonton (Canada)
Country of Origin:
Canada
Language:
English
Availability:
National Library of Canada, Ottawa.
Submitting Site:
JMT
Size:
Pages: v
Announcement Date:

Thesis/Dissertation:

Citation Formats

Fuciarelli, A F. Molecular radiobiology of nucleic acids. Canada: N. p., 1987. Web.
Fuciarelli, A F. Molecular radiobiology of nucleic acids. Canada.
Fuciarelli, A F. 1987. "Molecular radiobiology of nucleic acids." Canada.
@misc{etde_6856619,
title = {Molecular radiobiology of nucleic acids}
author = {Fuciarelli, A F}
abstractNote = {In addition to radiolytic adenine release, radiolysis of adenosine 5'-monophosphate, in the absence of oxygen, can result in the formation of 8-hydroxyadenosine 5'-monophosphate and both the (R)- and (S)-epimer of 8,5'-cycloadenosine 5'-monophosphate. The mononucleoside derivatives of these modified nucleotides were also observed in irradiated solutions of adenosine and in the enzyme hydrolysates of irradiated solutions of polyadenylic acid (poly A) using high-performance liquid chromatography (HPLC). In an effort to detect 8,5'-cyclo(deoxy) adenosine formation in irradiated nucleic acids, polyclonal antiserum were raised with specificity to the 8,5'-cycloadenosine 5'-monophosphate moiety and used in an enzyme-linked immunosorbent assay (ELISA). The 8,5'-cyclo(deoxy)adenosine moiety could be detected in nitrous oxide-saturated aqueous solutions containing unhydrolyzed poly A at 10 Gy and DNA at 200 Gy using the colorimetric ELISA. Correlation of product yield measured by ELISA with HPLC analysis of irradiated, enzyme-hydrolyzed solutions of poly A revealed that the ELISA was precisely reflecting changes in the combined yield of (R)- and (S)-8,5'-cycloadenosine.}
place = {Canada}
year = {1987}
month = {Jan}
}