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Anamnesis of the radioimmunoassay: A historical reminiscence

Journal Article:

Abstract

Anamnesis of the Radioimmunoassay - A Historical Reminiscence: Several requirements have to be met before the first radioimmunoassay of a proteohormone (i.e., insulin) could be developed. One of these requirements was that iodination ought not substantially impair the immunologic properties of the insulin molecule. Since the reversibility of antigen adsorption to antibodies is the most important basis of all immunoassays, the adsorptive behavior of proteins first had to be studied at extremely low protein concentrations. Only after these requirements had been investigated was direct electrophoretic detection of a nonprecipitating anti-hormone antibody possible by desorption of insulin on filter paper. Later, the inaccuracy of the species specificity of insulin-binding antibodies proved to be particularly useful for the determination of insulin in human sera.
Authors:
Publication Date:
Jan 27, 1984
Product Type:
Journal Article
Reference Number:
EDB-84-094793
Resource Relation:
Journal Name: Muench. Med. Wochenschr.; (Germany, Federal Republic of); Journal Volume: 126:4
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; 59 BASIC BIOLOGICAL SCIENCES; INSULIN; RADIOIMMUNOASSAY; ANTIBODIES; ANTIGENS; BLOOD SERUM; IODINE 131; LABELLED COMPOUNDS; SENSITIVITY; SPECIFICITY; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; DAYS LIVING RADIOISOTOPES; HORMONES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; PEPTIDE HORMONES; RADIOASSAY; RADIOISOTOPES; TRACER TECHNIQUES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics; 550501 - Metabolism- Tracer Techniques
OSTI ID:
6834229
Country of Origin:
Germany
Language:
German
Other Identifying Numbers:
Journal ID: CODEN: MMWOA
Submitting Site:
HEDB
Size:
Pages: 97-101
Announcement Date:
Apr 01, 1984

Journal Article:

Citation Formats

Kallee, E. Anamnesis of the radioimmunoassay: A historical reminiscence. Germany: N. p., 1984. Web.
Kallee, E. Anamnesis of the radioimmunoassay: A historical reminiscence. Germany.
Kallee, E. 1984. "Anamnesis of the radioimmunoassay: A historical reminiscence." Germany.
@misc{etde_6834229,
title = {Anamnesis of the radioimmunoassay: A historical reminiscence}
author = {Kallee, E}
abstractNote = {Anamnesis of the Radioimmunoassay - A Historical Reminiscence: Several requirements have to be met before the first radioimmunoassay of a proteohormone (i.e., insulin) could be developed. One of these requirements was that iodination ought not substantially impair the immunologic properties of the insulin molecule. Since the reversibility of antigen adsorption to antibodies is the most important basis of all immunoassays, the adsorptive behavior of proteins first had to be studied at extremely low protein concentrations. Only after these requirements had been investigated was direct electrophoretic detection of a nonprecipitating anti-hormone antibody possible by desorption of insulin on filter paper. Later, the inaccuracy of the species specificity of insulin-binding antibodies proved to be particularly useful for the determination of insulin in human sera.}
journal = {Muench. Med. Wochenschr.; (Germany, Federal Republic of)}
volume = {126:4}
journal type = {AC}
place = {Germany}
year = {1984}
month = {Jan}
}