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Multiple stable isotope tracer technique for studying the metabolic kinetics of amino acids in hepatic failure

Journal Article:

Abstract

In order to study the mechanism of the imbalance of amino acid metabolism during hepatic failure, a stable isotope tracer method for observing simultaneously the metabolic kinetics of several amino acids has been established. /sup 15/N-L-Ala, (2,3-D/sub 3/)-Leu and (2,3-D/sub 3/)-Phe were chosen as nonessential, branched chain and aromatic amino acids. A single iv injection of 40 mg N-Ala, 20 mg deuterated Leu and 20 mg deuterated Phe was given to each human subject. Blood samples were taken just before and at different times (up to 60 min) after the injection. Total free amino acids were isolated from the plasma with a small dowex 50 x 8 column and converted to trifluoroacetyl derivatives. Their abundances were then analyzed with a GC-MS system and typical double exponential time course curves were found for all the three labelled amino acids. A two-pool model was designed and applied for compartmental analysis. Significant changes were found in the kinetic parameters of Phe and Leu in patients with fulminant hepatitis or heptic cirrhosis. The half-lives of both Phe pools were longer and the pool sizes were larger than normal subjects, while the half-lives and pool sizes of Leu changes in the opposite direction. No marked  More>>
Publication Date:
Aug 01, 1987
Product Type:
Journal Article
Reference Number:
AIX-19-088992; EDB-88-163694
Resource Relation:
Journal Name: Hejishu; (China); Journal Volume: 10:8
Subject:
59 BASIC BIOLOGICAL SCIENCES; ALANINES; METABOLISM; HEPATITIS; LEUCINE; PHENYLALANINE; BIOCHEMICAL REACTION KINETICS; BIOLOGICAL PATHWAYS; DEUTERIUM; GAS CHROMATOGRAPHY; LABELLED COMPOUNDS; MASS SPECTROSCOPY; NITROGEN 15; TRACER TECHNIQUES; VIRAL DISEASES; AMINO ACIDS; CARBOXYLIC ACIDS; CHROMATOGRAPHY; DIGESTIVE SYSTEM DISEASES; DISEASES; HYDROGEN ISOTOPES; INFECTIOUS DISEASES; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; LIGHT NUCLEI; NITROGEN ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; ODD-ODD NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; REACTION KINETICS; SEPARATION PROCESSES; SPECTROSCOPY; STABLE ISOTOPES; 550501* - Metabolism- Tracer Techniques; 550901 - Pathology- Tracer Techniques
OSTI ID:
6828608
Research Organizations:
Shanghai Second Medical Univ. (China); Shanghai Ruijin Hospital (China)
Country of Origin:
China
Language:
Chinese
Other Identifying Numbers:
Journal ID: CODEN: NUTED
Submitting Site:
INIS
Size:
Pages: 45-53
Announcement Date:

Journal Article:

Citation Formats

Zongqin, Xia, Tengchang, Dai, Jianhua, Zhang, Yaer, Hu, Bingyao, Yu, Xingrong, Xu, Guanlu, Huang, Gengrong, Shen, Yaqiu, Zhou, and Hong, Yu. Multiple stable isotope tracer technique for studying the metabolic kinetics of amino acids in hepatic failure. China: N. p., 1987. Web.
Zongqin, Xia, Tengchang, Dai, Jianhua, Zhang, Yaer, Hu, Bingyao, Yu, Xingrong, Xu, Guanlu, Huang, Gengrong, Shen, Yaqiu, Zhou, & Hong, Yu. Multiple stable isotope tracer technique for studying the metabolic kinetics of amino acids in hepatic failure. China.
Zongqin, Xia, Tengchang, Dai, Jianhua, Zhang, Yaer, Hu, Bingyao, Yu, Xingrong, Xu, Guanlu, Huang, Gengrong, Shen, Yaqiu, Zhou, and Hong, Yu. 1987. "Multiple stable isotope tracer technique for studying the metabolic kinetics of amino acids in hepatic failure." China.
@misc{etde_6828608,
title = {Multiple stable isotope tracer technique for studying the metabolic kinetics of amino acids in hepatic failure}
author = {Zongqin, Xia, Tengchang, Dai, Jianhua, Zhang, Yaer, Hu, Bingyao, Yu, Xingrong, Xu, Guanlu, Huang, Gengrong, Shen, Yaqiu, Zhou, and Hong, Yu}
abstractNote = {In order to study the mechanism of the imbalance of amino acid metabolism during hepatic failure, a stable isotope tracer method for observing simultaneously the metabolic kinetics of several amino acids has been established. /sup 15/N-L-Ala, (2,3-D/sub 3/)-Leu and (2,3-D/sub 3/)-Phe were chosen as nonessential, branched chain and aromatic amino acids. A single iv injection of 40 mg N-Ala, 20 mg deuterated Leu and 20 mg deuterated Phe was given to each human subject. Blood samples were taken just before and at different times (up to 60 min) after the injection. Total free amino acids were isolated from the plasma with a small dowex 50 x 8 column and converted to trifluoroacetyl derivatives. Their abundances were then analyzed with a GC-MS system and typical double exponential time course curves were found for all the three labelled amino acids. A two-pool model was designed and applied for compartmental analysis. Significant changes were found in the kinetic parameters of Phe and Leu in patients with fulminant hepatitis or heptic cirrhosis. The half-lives of both Phe pools were longer and the pool sizes were larger than normal subjects, while the half-lives and pool sizes of Leu changes in the opposite direction. No marked change was found in Ala. The significance of intracellular imbalance of Phe and Leu metabolism was discussed. It is evident that the combination of GCMS technique and multiple-tracers labelled with stable isotopes is of great potential for similar purposes.}
journal = {Hejishu; (China)}
volume = {10:8}
journal type = {AC}
place = {China}
year = {1987}
month = {Aug}
}