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Investigation on the absorption of /sup 14/C-leucine and /sup 15/N-leucine in rats after feeding a fish meal diet in comparison with a gelatine diet

Journal Article:

Abstract

Albino rats received after nine days of adaptation to a fish meal diet in comparison with a gelatine diet /sup 14/C-U-L-leucine and /sup 15/N-L-leucine via a pellet made from the specific diet after food deprivation for 15 h. Thereafter, the animals consumed the non-labelled experimental diet ad libitum. 30 min, and 1, 2, 4 and 8 h, resp., after intake of the labelled food, four rats at a time were sacrificed. The contents of the digestive tract and tissue samples were examined for /sup 14/C and /sup 15/N and their percentages in the TCA-soluble fraction determined. If these values are regarded as non-absorbed leucine, the /sup 14/C values obtained up to the four hour period of the experiment would be too high. Presumably, they are in the case of both diets simulated by other /sup 14/C metabolites which originate from the leucine catabolism and reach the intestinal lumen. Amino acids labelled with /sup 15/N should be preferred in studies on the absorption of amino acids because, in case of catabolization, the /sup 15/N aminogroup is excreted mainly as urea via urine.
Authors:
Bergner, U; Adam, K; Bergner, H [1] 
  1. Humboldt-Universitaet, Berlin (German Democratic Republic). Sektion Tierproduktion und Veterinaermedizin
Publication Date:
Jan 01, 1981
Product Type:
Journal Article
Reference Number:
AIX-13-699197; EDB-82-172504
Resource Relation:
Journal Name: Arch. Tierernaehr.; (German Democratic Republic); Journal Volume: 31:7-8
Subject:
59 BASIC BIOLOGICAL SCIENCES; LEUCINE; INTESTINAL ABSORPTION; BIOLOGICAL LOCALIZATION; CARBON 14 COMPOUNDS; CATABOLISM; DIET; FECES; FISH PRODUCTS; GELATIN; LABELLED COMPOUNDS; LARGE INTESTINE; METABOLITES; NITROGEN 15; RATS; SMALL INTESTINE; STOMACH; TIME DEPENDENCE; TRACER TECHNIQUES; ABSORPTION; AMINO ACIDS; ANIMALS; BIOLOGICAL MATERIALS; BIOLOGICAL WASTES; BODY; CARBOXYLIC ACIDS; COLLOIDS; DIGESTIVE SYSTEM; DISPERSIONS; GASTROINTESTINAL TRACT; INTESTINES; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; MAMMALS; MATERIALS; METABOLISM; NITROGEN ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RODENTS; STABLE ISOTOPES; UPTAKE; VERTEBRATES; WASTES; 550501* - Metabolism- Tracer Techniques
OSTI ID:
6816967
Country of Origin:
Germany
Language:
German
Other Identifying Numbers:
Journal ID: CODEN: ARTIA
Submitting Site:
HEDB
Size:
Pages: 447-459
Announcement Date:
Sep 01, 1982

Journal Article:

Citation Formats

Bergner, U, Adam, K, and Bergner, H. Investigation on the absorption of /sup 14/C-leucine and /sup 15/N-leucine in rats after feeding a fish meal diet in comparison with a gelatine diet. Germany: N. p., 1981. Web.
Bergner, U, Adam, K, & Bergner, H. Investigation on the absorption of /sup 14/C-leucine and /sup 15/N-leucine in rats after feeding a fish meal diet in comparison with a gelatine diet. Germany.
Bergner, U, Adam, K, and Bergner, H. 1981. "Investigation on the absorption of /sup 14/C-leucine and /sup 15/N-leucine in rats after feeding a fish meal diet in comparison with a gelatine diet." Germany.
@misc{etde_6816967,
title = {Investigation on the absorption of /sup 14/C-leucine and /sup 15/N-leucine in rats after feeding a fish meal diet in comparison with a gelatine diet}
author = {Bergner, U, Adam, K, and Bergner, H}
abstractNote = {Albino rats received after nine days of adaptation to a fish meal diet in comparison with a gelatine diet /sup 14/C-U-L-leucine and /sup 15/N-L-leucine via a pellet made from the specific diet after food deprivation for 15 h. Thereafter, the animals consumed the non-labelled experimental diet ad libitum. 30 min, and 1, 2, 4 and 8 h, resp., after intake of the labelled food, four rats at a time were sacrificed. The contents of the digestive tract and tissue samples were examined for /sup 14/C and /sup 15/N and their percentages in the TCA-soluble fraction determined. If these values are regarded as non-absorbed leucine, the /sup 14/C values obtained up to the four hour period of the experiment would be too high. Presumably, they are in the case of both diets simulated by other /sup 14/C metabolites which originate from the leucine catabolism and reach the intestinal lumen. Amino acids labelled with /sup 15/N should be preferred in studies on the absorption of amino acids because, in case of catabolization, the /sup 15/N aminogroup is excreted mainly as urea via urine.}
journal = {Arch. Tierernaehr.; (German Democratic Republic)}
volume = {31:7-8}
journal type = {AC}
place = {Germany}
year = {1981}
month = {Jan}
}