You need JavaScript to view this

Enzymatic radioiodination of insulin for radioimmunoassay use

Journal Article:

Abstract

Insulin was labelled with /sup 125/I using lactoperoxidase as an oxidizing agent. The reaction product was purified via two stages; a starch gel electrophoresis(SGE) and a Sephadex gel filtration(SF). Upon comparison of the labelling yields and the bindabilities of the labelled insulin to its antibody, it has been found that the enzyme method shows higher yields (50%) and the better bindability to its antibody than the conventional chloramine-T method (35%). By checking the insulin blank labelling mixture with a SGE, a paper chromatography, and a radioautography technique, a by-product in the lactoperoxidase method has been identified. The separated fractions in SGE and SF were also analyzed and discussed.
Authors:
Awh, O D; Kim, J R [1] 
  1. Korea Atomic Energy Research Inst., Seoul (Republic of Korea)
Publication Date:
Jun 01, 1980
Product Type:
Journal Article
Reference Number:
AIX-11-561745; EDB-80-129155
Resource Relation:
Journal Name: J. Korean Nucl. Soc.; (Korea, South); Journal Volume: 12:2
Subject:
38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY; INSULIN; LABELLING; ANTIBODIES; ANTIGEN-ANTIBODY REACTIONS; CHEMICAL REACTION YIELD; CHLORAMINES; ELECTROPHORESIS; FILTRATION; IODINE; PEROXIDES; RADIOIMMUNOASSAY; AMINES; ELEMENTS; HALOGENS; HORMONES; ISOTOPE APPLICATIONS; NONMETALS; ORGANIC CHLORINE COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; OXYGEN COMPOUNDS; PEPTIDE HORMONES; RADIOASSAY; SEPARATION PROCESSES; TRACER TECHNIQUES; YIELDS; 400702* - Radiochemistry & Nuclear Chemistry- Properties of Radioactive Materials
OSTI ID:
6811306
Country of Origin:
Korea, Republic of
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: KNSJA
Submitting Site:
INIS
Size:
Pages: 81-87
Announcement Date:
Dec 01, 1980

Journal Article:

Citation Formats

Awh, O D, and Kim, J R. Enzymatic radioiodination of insulin for radioimmunoassay use. Korea, Republic of: N. p., 1980. Web.
Awh, O D, & Kim, J R. Enzymatic radioiodination of insulin for radioimmunoassay use. Korea, Republic of.
Awh, O D, and Kim, J R. 1980. "Enzymatic radioiodination of insulin for radioimmunoassay use." Korea, Republic of.
@misc{etde_6811306,
title = {Enzymatic radioiodination of insulin for radioimmunoassay use}
author = {Awh, O D, and Kim, J R}
abstractNote = {Insulin was labelled with /sup 125/I using lactoperoxidase as an oxidizing agent. The reaction product was purified via two stages; a starch gel electrophoresis(SGE) and a Sephadex gel filtration(SF). Upon comparison of the labelling yields and the bindabilities of the labelled insulin to its antibody, it has been found that the enzyme method shows higher yields (50%) and the better bindability to its antibody than the conventional chloramine-T method (35%). By checking the insulin blank labelling mixture with a SGE, a paper chromatography, and a radioautography technique, a by-product in the lactoperoxidase method has been identified. The separated fractions in SGE and SF were also analyzed and discussed.}
journal = {J. Korean Nucl. Soc.; (Korea, South)}
volume = {12:2}
journal type = {AC}
place = {Korea, Republic of}
year = {1980}
month = {Jun}
}