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Continuous cell recycle fermentation

Technical Report:

Abstract

A cell recycle fermentor using a cross-flow membrane filter has been operated for extended periods. Productivities as high as 70 g/l/h were obtained at a cell concentration of 120 g/l and a product concentration of 70 g/l. The experimental results were then fitted to previously derived biokinetic models (Warren et al., 1990) for a continuous stirred tank fermentor. A good fit for growth rate was found and the cell yield was shown to decrease with product concentration. The product yield, however, was found to remain nearly constant at all cell, substrate and product concentrations. These biokinetics, along with a previous model for the membrane filter (Warren et al., 1991) were then used in a simulalation to estimate the costs of producing ethanol in a large scale system. This simulation was optimized using a variant of the steepest descent method from which a fermentor inlet substrate concentration of 150 g/l and a net cost of $CAN 253.5/1000 L ethanol were projected. From a sensitivity analysis, the yield parameters were found to have the greatest effect on ethanol net cost of the fermentor parameters, while the operating costs and the profit was found to be most sensitive to the wheat raw material  More>>
Publication Date:
Oct 01, 1991
Product Type:
Technical Report
Report Number:
SU/CE-91-7/180-1992E; MICROLOG-92-06732
Reference Number:
CANM-93-0E5705; EDB-93-075146
Subject:
09 BIOMASS FUELS; BIOMASS; FERMENTATION; ETHANOL; PRODUCTION; CELL RECYCLE; CONTINUOUS CULTURE; CHEMICAL REACTION YIELD; COST; SACCHAROMYCES CEREVISIAE; SENSITIVITY ANALYSIS; ALCOHOLS; BIOCONVERSION; ENERGY SOURCES; EUMYCOTA; FUNGI; HYDROXY COMPOUNDS; MICROORGANISMS; ORGANIC COMPOUNDS; PLANTS; RENEWABLE ENERGY SOURCES; SACCHAROMYCES; YEASTS; YIELDS; 090900* - Biomass Fuels- Processing- (1990-)
Sponsoring Organizations:
PERD; EMR; Federal Panel on Energy Research and Development, Ottawa, ON (Canada); Department of Energy, Mines and Resources, Ottawa, ON (Canada)
OSTI ID:
6806821
Research Organizations:
Saskatchewan Univ., Saskatoon, SK (Canada). Dept. of Chemical Engineering
Country of Origin:
Canada
Language:
English
Other Identifying Numbers:
Other: CNN: EMR 23440-0-9569; ISBN: 0-662-19742-9
Availability:
PC Canada Centre for Mineral and Energy Technology, Technical Information Division, 562 Booth St., Room 20-C, Ottawa, ON, CAN K1A 0G1; MF CANMET/TID, Energy, Mines and Resources Canada, 555 Booth St., Ottawa, Ont., Canada K1A 0G1 PC
Submitting Site:
CANM
Size:
Pages: (94 p)
Announcement Date:
May 13, 2001

Technical Report:

Citation Formats

Warren, R K, Hill, G A, and MacDonald, D G. Continuous cell recycle fermentation. Canada: N. p., 1991. Web.
Warren, R K, Hill, G A, & MacDonald, D G. Continuous cell recycle fermentation. Canada.
Warren, R K, Hill, G A, and MacDonald, D G. 1991. "Continuous cell recycle fermentation." Canada.
@misc{etde_6806821,
title = {Continuous cell recycle fermentation}
author = {Warren, R K, Hill, G A, and MacDonald, D G}
abstractNote = {A cell recycle fermentor using a cross-flow membrane filter has been operated for extended periods. Productivities as high as 70 g/l/h were obtained at a cell concentration of 120 g/l and a product concentration of 70 g/l. The experimental results were then fitted to previously derived biokinetic models (Warren et al., 1990) for a continuous stirred tank fermentor. A good fit for growth rate was found and the cell yield was shown to decrease with product concentration. The product yield, however, was found to remain nearly constant at all cell, substrate and product concentrations. These biokinetics, along with a previous model for the membrane filter (Warren et al., 1991) were then used in a simulalation to estimate the costs of producing ethanol in a large scale system. This simulation was optimized using a variant of the steepest descent method from which a fermentor inlet substrate concentration of 150 g/l and a net cost of $CAN 253.5/1000 L ethanol were projected. From a sensitivity analysis, the yield parameters were found to have the greatest effect on ethanol net cost of the fermentor parameters, while the operating costs and the profit was found to be most sensitive to the wheat raw material cost and to the dried grains by-product value. 55 refs., 11 tabs., 7figs.}
place = {Canada}
year = {1991}
month = {Oct}
}