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Blood cell labeling with technetium-99m. II. Measurement of circulating blood volume by sup(99m)Tc-labeled red blood cells

Journal Article:

Abstract

Using a labeling method with sup(99m)Tc-pertechnetate to red blood cells (RBC), circulating blood volume was measured in comparison with that from /sup 51/Cr-labeled RBC method. The technique is easier than already published methods, because CIS kit for sup(99m)Tc-RBC labeling (TCK-11) became to be available recently. Two mls of ACD-anticoagulated blood were withdrawn and 0.5 ml of reducing reagent prepared just before use was added to blood, waiting 5 minutes and discarding the serum after centrifugation, then adding 100 ..mu..Ci of sup(99m)Tc. After washing the labeled cells by isotonic saline, cells were re-suspended in 10 ml of saline and injected to the subject. Blood specimen was obtained 10, 30, 60 and 120 minutes after infusion and blood volume was calculated by the usual way. Circulating blood volume by sup(99m)Tc was well correlated with that by /sup 51/Cr (=0.98, p 0.01), however, the value calculated from sup(99m)Tc were 4.8 percent higher than those by /sup 51/Cr, which suggested the elution of sup(99m)Tc from labeled RBC. sup(99m)Tc method has the advantages that higher radioactivity can be obtained in small amount of blood, which is useful in the determination of blood volume in children or in small animals in the laboratory. The measurement of  More>>
Authors:
Uchida, T; Yoshida, H; Matsuda, S; Kimura, H; Miura, N [1] 
  1. Fukushima Medical Coll. (Japan)
Publication Date:
Feb 01, 1978
Product Type:
Journal Article
Reference Number:
AIX-09-411247; EDB-79-022045
Resource Relation:
Journal Name: Kaku Igaku; (Japan); Journal Volume: 15:1
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; 38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY; BLOOD; MEASURING METHODS; ERYTHROCYTES; LABELLING; RADIOPHARMACEUTICALS; COMPARATIVE EVALUATIONS; TECHNETIUM 99; RADIOCHEMISTRY; ANEMIAS; BLOOD COUNT; BLOOD FLOW; CHROMIUM 51; IN VIVO; MICE; PATIENTS; RADIOACTIVITY; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD CELLS; BODY FLUIDS; CHEMISTRY; CHROMIUM ISOTOPES; DISEASES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; EVEN-ODD NUCLEI; HEMIC DISEASES; HOURS LIVING RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; LABELLED COMPOUNDS; MAMMALS; NUCLEI; ODD-EVEN NUCLEI; RADIOACTIVE MATERIALS; RADIOISOTOPES; RODENTS; SYMPTOMS; TECHNETIUM ISOTOPES; VERTEBRATES; YEARS LIVING RADIOISOTOPES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics; 400702 - Radiochemistry & Nuclear Chemistry- Properties of Radioactive Materials
OSTI ID:
6802587
Country of Origin:
Japan
Language:
Japanese
Other Identifying Numbers:
Journal ID: CODEN: KAIGB
Submitting Site:
INIS
Size:
Pages: 41-47
Announcement Date:

Journal Article:

Citation Formats

Uchida, T, Yoshida, H, Matsuda, S, Kimura, H, and Miura, N. Blood cell labeling with technetium-99m. II. Measurement of circulating blood volume by sup(99m)Tc-labeled red blood cells. Japan: N. p., 1978. Web.
Uchida, T, Yoshida, H, Matsuda, S, Kimura, H, & Miura, N. Blood cell labeling with technetium-99m. II. Measurement of circulating blood volume by sup(99m)Tc-labeled red blood cells. Japan.
Uchida, T, Yoshida, H, Matsuda, S, Kimura, H, and Miura, N. 1978. "Blood cell labeling with technetium-99m. II. Measurement of circulating blood volume by sup(99m)Tc-labeled red blood cells." Japan.
@misc{etde_6802587,
title = {Blood cell labeling with technetium-99m. II. Measurement of circulating blood volume by sup(99m)Tc-labeled red blood cells}
author = {Uchida, T, Yoshida, H, Matsuda, S, Kimura, H, and Miura, N}
abstractNote = {Using a labeling method with sup(99m)Tc-pertechnetate to red blood cells (RBC), circulating blood volume was measured in comparison with that from /sup 51/Cr-labeled RBC method. The technique is easier than already published methods, because CIS kit for sup(99m)Tc-RBC labeling (TCK-11) became to be available recently. Two mls of ACD-anticoagulated blood were withdrawn and 0.5 ml of reducing reagent prepared just before use was added to blood, waiting 5 minutes and discarding the serum after centrifugation, then adding 100 ..mu..Ci of sup(99m)Tc. After washing the labeled cells by isotonic saline, cells were re-suspended in 10 ml of saline and injected to the subject. Blood specimen was obtained 10, 30, 60 and 120 minutes after infusion and blood volume was calculated by the usual way. Circulating blood volume by sup(99m)Tc was well correlated with that by /sup 51/Cr (=0.98, p 0.01), however, the value calculated from sup(99m)Tc were 4.8 percent higher than those by /sup 51/Cr, which suggested the elution of sup(99m)Tc from labeled RBC. sup(99m)Tc method has the advantages that higher radioactivity can be obtained in small amount of blood, which is useful in the determination of blood volume in children or in small animals in the laboratory. The measurement of blood volume of the mouse was done by using sup(99m)Tc method. The results were 1.70 +- 0.06 ml (6.35 +- 0.18%/gm), which coincided with the values reported previously. Because of it's short half life and low radiation dosage to the patients, sup(99m)Tc method will be recommended in the field of pediatrics or in patients with polycythemia or congestive heart failure, who are requested the repeated measurement of blood volume.}
journal = {Kaku Igaku; (Japan)}
volume = {15:1}
journal type = {AC}
place = {Japan}
year = {1978}
month = {Feb}
}