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New method of isolation and perfusion of rat pancreas for phosphorus nucletic resonance study

Journal Article:

Abstract

Phosphorus nuclear magnetic resonance (/sup 31/P-NMR) was applied to measure phosphorus metabolites in the pancreas. Rat pancreatic gland was isolated and perfused with a modified Krebs-Henseleit solution at room temperature (25 deg C). Removal of the duodenum from the pancreas made it possible to record /sup 31/P-NMR spectra only from the pancreatic tissue. /sup 31/P signals of creatine phosphate (PCr), ATP and inorganic phosphate (Pi) were observed in all the glands. In different individual glands, the Signal intensity of PCr varied, but those of ATP were almost the same. The initial levels of all the phosphorus metabolites in individual glands remained almost unchanged during perfusion with the standard solution for 2 hours. When the perfusion was stopped, signal intensity of ATP and PCr decreased while Pi resonance increased and shifted to a higher magnetic field, indicating tissue acidification. On re-perfusion, each resonance and tissue pH were restored to the respective initial resting levels. Continuous stimulation with 0.3 ..mu..M acetylcholine (ACh) or 0.1 nM CCK-8 caused a markedly larger increase in pancreatic exocrine secretion than that observed in the perfused pancreas which was prepared by the previous method. Continuous stimulation with 0.1 ..mu..M ACh caused a marked and sustained increase in  More>>
Publication Date:
Feb 01, 1988
Product Type:
Journal Article
Reference Number:
JPN-89-001187; EDB-89-022405
Resource Relation:
Journal Name: NMR Igaku; (Japan); Journal Volume: 7:4
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; PANCREAS; NMR SPECTRA; PHOSPHORUS 31; ACETYLCHOLINE; ENERGY SPECTRA; METABOLISM; PERFUSED ORGANS; RATS; AMINES; AMMONIUM COMPOUNDS; ANIMALS; AUTONOMIC NERVOUS SYSTEM AGENTS; BODY; DIGESTIVE SYSTEM; DRUGS; ENDOCRINE GLANDS; ESTERS; GLANDS; ISOTOPES; LIGHT NUCLEI; MAMMALS; NEUROREGULATORS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PARASYMPATHOMIMETICS; PHOSPHORUS ISOTOPES; QUATERNARY COMPOUNDS; RODENTS; SPECTRA; STABLE ISOTOPES; VERTEBRATES; 550602* - Medicine- External Radiation in Diagnostics- (1980-)
OSTI ID:
6798483
Research Organizations:
Hokkaido Univ., Sapporo (Japan). School of Medicine
Country of Origin:
Japan
Language:
Japanese
Other Identifying Numbers:
Journal ID: CODEN: NMRIE
Submitting Site:
JPN
Size:
Pages: 223-232
Announcement Date:
Jun 01, 1988

Journal Article:

Citation Formats

Matsumoto, Takehisa, Kanno, Tomio, Murakami, Masataka, Watari, Hiroshi, and Seo, Yoshiteru. New method of isolation and perfusion of rat pancreas for phosphorus nucletic resonance study. Japan: N. p., 1988. Web.
Matsumoto, Takehisa, Kanno, Tomio, Murakami, Masataka, Watari, Hiroshi, & Seo, Yoshiteru. New method of isolation and perfusion of rat pancreas for phosphorus nucletic resonance study. Japan.
Matsumoto, Takehisa, Kanno, Tomio, Murakami, Masataka, Watari, Hiroshi, and Seo, Yoshiteru. 1988. "New method of isolation and perfusion of rat pancreas for phosphorus nucletic resonance study." Japan.
@misc{etde_6798483,
title = {New method of isolation and perfusion of rat pancreas for phosphorus nucletic resonance study}
author = {Matsumoto, Takehisa, Kanno, Tomio, Murakami, Masataka, Watari, Hiroshi, and Seo, Yoshiteru}
abstractNote = {Phosphorus nuclear magnetic resonance (/sup 31/P-NMR) was applied to measure phosphorus metabolites in the pancreas. Rat pancreatic gland was isolated and perfused with a modified Krebs-Henseleit solution at room temperature (25 deg C). Removal of the duodenum from the pancreas made it possible to record /sup 31/P-NMR spectra only from the pancreatic tissue. /sup 31/P signals of creatine phosphate (PCr), ATP and inorganic phosphate (Pi) were observed in all the glands. In different individual glands, the Signal intensity of PCr varied, but those of ATP were almost the same. The initial levels of all the phosphorus metabolites in individual glands remained almost unchanged during perfusion with the standard solution for 2 hours. When the perfusion was stopped, signal intensity of ATP and PCr decreased while Pi resonance increased and shifted to a higher magnetic field, indicating tissue acidification. On re-perfusion, each resonance and tissue pH were restored to the respective initial resting levels. Continuous stimulation with 0.3 ..mu..M acetylcholine (ACh) or 0.1 nM CCK-8 caused a markedly larger increase in pancreatic exocrine secretion than that observed in the perfused pancreas which was prepared by the previous method. Continuous stimulation with 0.1 ..mu..M ACh caused a marked and sustained increase in pancreatic exocrine secretion. During the stimulation, the resonances of phosphorus energy metabolites and tissue pH remained almost unchanged. These results indicate that the isolated perfused rat pancreas which is prepared by our new method is a useful preparation for analysis of secretion-metabolism coupling in the exocrine pancreas.}
journal = {NMR Igaku; (Japan)}
volume = {7:4}
journal type = {AC}
place = {Japan}
year = {1988}
month = {Feb}
}