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DNA-membrane complex restoration in Micrococcus radiodurans after X-irradiation: relation to repair, DNA synthesis and DNA degradation

Abstract

The DNA-membrane complex in Micrococcus radiodurans was shown to be essentially constituted of proteins, lipids and DNA. The complex was dissociated immediately after X-irradiation of cells and restored during post-incubation in complete medium. In X-irradiated protoplasts some DNA remained associated with the complex. Restoration of the complex during post-incubation was only seen in a medium favouring DNA polymerase and ligase activities. Under this condition no DNA synthesis occurred, suggesting that complex restoration may involve ligase activity. The complex restoration in the wild type and the X-ray sensitive mutant UV17 of M. radiodurans was strictly dependent on the X-ray dose. It was correlated with survival and DNA degradation but always preceded the onset of DNA synthesis after X-irradiation. At the same dose the complex restoration was about 2 fold lower in mutant than in wild type cells indicating that the restoration of the complex is related to repair capacity. The results are consistent with the idea that the complex protects X-irradiated DNA of M. radiodurans from further breakdown and, subsequently, permits DNA synthesis and repair to occur.
Authors:
Dardalhon-Samsonoff, M; Averbeck, D [1] 
  1. Institut du Radium, 75 - Paris (France). Lab. Curie
Publication Date:
Jul 01, 1980
Product Type:
Journal Article
Reference Number:
AIX-11-561960; EDB-81-006878
Resource Relation:
Journal Name: Int. J. Radiat. Biol.; (United Kingdom); Journal Volume: 38:1
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DNA; BIOLOGICAL REPAIR; MICROCOCCUS RADIODURANS; BIOLOGICAL RADIATION EFFECTS; BIOSYNTHESIS; COMPLEXES; DOSE RATES; X RADIATION; BACTERIA; BIOLOGICAL EFFECTS; BIOLOGICAL RECOVERY; ELECTROMAGNETIC RADIATION; IONIZING RADIATIONS; MICROCOCCUS; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; RADIATION EFFECTS; RADIATIONS; RECOVERY; REPAIR; SYNTHESIS; 560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987); 560112 - Radiation Effects on Biochemicals- In Microorganisms- (-1987)
OSTI ID:
6784511
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: IJRBA
Submitting Site:
INIS
Size:
Pages: 31-52
Announcement Date:

Citation Formats

Dardalhon-Samsonoff, M, and Averbeck, D. DNA-membrane complex restoration in Micrococcus radiodurans after X-irradiation: relation to repair, DNA synthesis and DNA degradation. United Kingdom: N. p., 1980. Web. doi:10.1080/09553008014550931.
Dardalhon-Samsonoff, M, & Averbeck, D. DNA-membrane complex restoration in Micrococcus radiodurans after X-irradiation: relation to repair, DNA synthesis and DNA degradation. United Kingdom. doi:10.1080/09553008014550931.
Dardalhon-Samsonoff, M, and Averbeck, D. 1980. "DNA-membrane complex restoration in Micrococcus radiodurans after X-irradiation: relation to repair, DNA synthesis and DNA degradation." United Kingdom. doi:10.1080/09553008014550931. https://www.osti.gov/servlets/purl/10.1080/09553008014550931.
@misc{etde_6784511,
title = {DNA-membrane complex restoration in Micrococcus radiodurans after X-irradiation: relation to repair, DNA synthesis and DNA degradation}
author = {Dardalhon-Samsonoff, M, and Averbeck, D}
abstractNote = {The DNA-membrane complex in Micrococcus radiodurans was shown to be essentially constituted of proteins, lipids and DNA. The complex was dissociated immediately after X-irradiation of cells and restored during post-incubation in complete medium. In X-irradiated protoplasts some DNA remained associated with the complex. Restoration of the complex during post-incubation was only seen in a medium favouring DNA polymerase and ligase activities. Under this condition no DNA synthesis occurred, suggesting that complex restoration may involve ligase activity. The complex restoration in the wild type and the X-ray sensitive mutant UV17 of M. radiodurans was strictly dependent on the X-ray dose. It was correlated with survival and DNA degradation but always preceded the onset of DNA synthesis after X-irradiation. At the same dose the complex restoration was about 2 fold lower in mutant than in wild type cells indicating that the restoration of the complex is related to repair capacity. The results are consistent with the idea that the complex protects X-irradiated DNA of M. radiodurans from further breakdown and, subsequently, permits DNA synthesis and repair to occur.}
doi = {10.1080/09553008014550931}
journal = {Int. J. Radiat. Biol.; (United Kingdom)}
volume = {38:1}
journal type = {AC}
place = {United Kingdom}
year = {1980}
month = {Jul}
}