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Certain patterns of IgG adsorption by polystyrene bead surfaces

Abstract

The article reports on tests of domestic Soviet polystyrene beads that permit a simplified modification of the enzyme-adsorption method to identify the alpha hepatitis virus and its antibody in nonspecialized, general laboratories. Only patterns of Ig immunoglobulin adsorption were studied. Human IgG was conjugated with the radioactive isotope /sup 125/I by a chloramine method, with mean radioactivity and protein concentration measured frequently. Bovine serum albumin (BSA) and an anionic detergent Tween-20, and a phosphate-salt buffer with pH 5.8-8.2, were used to produce m-Ig and Ig. Adsorption involved incubation of the beads in various solutions, followed by measurement of their radioactivity. Results of several series of tests were subjected to Student-Fisher evaluation. This suggested that the presence of albumin in physiological concentrations in the solution had no important impact on m-Ig adsorption on the bead surface, which effectively adsorbed Ig from solutions without additional proteins, but also from Ig solutions containing serum albumin in physiological concentrations. Thus, it was possible to coat the beads with alpha Ig hepatitis virus. The Tween-20 weak detergent was effective for eliminating unwanted protein adsorption. 9 references, 3 figures.
Authors:
Publication Date:
Jan 01, 1985
Product Type:
Book
Reference Number:
EDB-87-049906
Resource Relation:
Other Information: JPRS-UBB--86-009
Subject:
59 BASIC BIOLOGICAL SCIENCES; IMMUNOGLOBULINS; ADSORPTION; POLYSTYRENE; VIRUSES; RADIOIMMUNOASSAY; ALBUMINS; ANTIBODIES; BLOOD SERUM; BUFFERS; CATTLE; CHLORAMINES; DETERGENTS; HEPATITIS; IODINE 125; LABELLED COMPOUNDS; PH VALUE; TRACER TECHNIQUES; ADDITIVES; AMINES; ANIMALS; BETA DECAY RADIOISOTOPES; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM DISEASES; DISEASES; DOMESTIC ANIMALS; ELECTRON CAPTURE RADIOISOTOPES; EMULSIFIERS; GLOBULINS; IMMUNOASSAY; IMMUNOLOGY; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; MATERIALS; MICROORGANISMS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC CHLORINE COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; ORGANIC POLYMERS; PARASITES; PETROCHEMICALS; PETROLEUM PRODUCTS; PLASTICS; POLYMERS; POLYOLEFINS; POLYVINYLS; PROTEINS; RADIOASSAY; RADIOIMMUNOLOGY; RADIOISOTOPES; RUMINANTS; SORPTION; SURFACTANTS; SYNTHETIC MATERIALS; VERTEBRATES; WETTING AGENTS; 551001* - Physiological Systems- Tracer Techniques
OSTI ID:
6777837
Country of Origin:
USSR
Language:
Russian
Submitting Site:
JMT
Size:
Pages: v
Announcement Date:

Citation Formats

Mamedov, M K. Certain patterns of IgG adsorption by polystyrene bead surfaces. USSR: N. p., 1985. Web.
Mamedov, M K. Certain patterns of IgG adsorption by polystyrene bead surfaces. USSR.
Mamedov, M K. 1985. "Certain patterns of IgG adsorption by polystyrene bead surfaces." USSR.
@misc{etde_6777837,
title = {Certain patterns of IgG adsorption by polystyrene bead surfaces}
author = {Mamedov, M K}
abstractNote = {The article reports on tests of domestic Soviet polystyrene beads that permit a simplified modification of the enzyme-adsorption method to identify the alpha hepatitis virus and its antibody in nonspecialized, general laboratories. Only patterns of Ig immunoglobulin adsorption were studied. Human IgG was conjugated with the radioactive isotope /sup 125/I by a chloramine method, with mean radioactivity and protein concentration measured frequently. Bovine serum albumin (BSA) and an anionic detergent Tween-20, and a phosphate-salt buffer with pH 5.8-8.2, were used to produce m-Ig and Ig. Adsorption involved incubation of the beads in various solutions, followed by measurement of their radioactivity. Results of several series of tests were subjected to Student-Fisher evaluation. This suggested that the presence of albumin in physiological concentrations in the solution had no important impact on m-Ig adsorption on the bead surface, which effectively adsorbed Ig from solutions without additional proteins, but also from Ig solutions containing serum albumin in physiological concentrations. Thus, it was possible to coat the beads with alpha Ig hepatitis virus. The Tween-20 weak detergent was effective for eliminating unwanted protein adsorption. 9 references, 3 figures.}
place = {USSR}
year = {1985}
month = {Jan}
}