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Cellulolytic properties of an extremely thermophilic anaerobe

Journal Article:

Abstract

An extremely thermophilic anaerobe was isolated from a New Zealand hot spring by incubating bacterial mat strands in a medium containing xylan. The Gramreaction-negative organism that was subsequently purified had a temperature optimum of 70deg C and a pH optimum of 7.0. The isolate, designated strain H173, grew on a restricted range of carbon sources. In batch culture H173 could degrade Avicel completely when supplied at 5 or 10 g l{sup -1}. There was an initial growth phase, during which a cellulase complex was produced and carbohydrates fermented to form acetic and lactic acids, followed by a phase where cells were not metabolising but the cellulase complex actively converted cellulose to glucose. When co-cultered with strain Rt8.B1, an ethanologenic extreme thermophile, glucose was fermented to ethanol and acetate, and no reducing sugars accumulated in the medium. In pH controlled batch culture H173 produced an increased amount of lactate and acetate but there was again a phase when reducing sugars accumulated in the medium, and these were converted to ethanol by co-culture with Rt8.B1. (orig.).
Authors:
Hudson, J A; Morgan, H W; Daniel, R M [1] 
  1. Waikato Univ., Hamilton (New Zealand). Microbial Biochemistry and Biotechnology Unit
Publication Date:
Sep 01, 1990
Product Type:
Journal Article
Reference Number:
DE-90-010093; EDB-90-163959
Resource Relation:
Journal Name: Applied Microbiology and Biotechnology; (Germany, F.R.); Journal Volume: 33:6
Subject:
09 BIOMASS FUELS; ENZYMES; CELLULOLYTIC ACTIVITY; PRODUCTION; ANAEROBIC CONDITIONS; BATCH CULTURE; BIOSYNTHESIS; ETHANOL; THERMOPHILIC CONDITIONS; XYLANS; ALCOHOLS; CARBOHYDRATES; ENZYME ACTIVITY; HEMICELLULOSE; HYDROXY COMPOUNDS; ORGANIC COMPOUNDS; POLYSACCHARIDES; SACCHARIDES; SYNTHESIS; 090900* - Biomass Fuels- Processing- (1990-)
OSTI ID:
6774864
Country of Origin:
Germany
Language:
English
Other Identifying Numbers:
Journal ID: ISSN 0175-7598; CODEN: AMBID
Submitting Site:
DE
Size:
Pages: 687-691
Announcement Date:
Nov 15, 1990

Journal Article:

Citation Formats

Hudson, J A, Morgan, H W, and Daniel, R M. Cellulolytic properties of an extremely thermophilic anaerobe. Germany: N. p., 1990. Web. doi:10.1007/BF00604939.
Hudson, J A, Morgan, H W, & Daniel, R M. Cellulolytic properties of an extremely thermophilic anaerobe. Germany. doi:10.1007/BF00604939.
Hudson, J A, Morgan, H W, and Daniel, R M. 1990. "Cellulolytic properties of an extremely thermophilic anaerobe." Germany. doi:10.1007/BF00604939. https://www.osti.gov/servlets/purl/10.1007/BF00604939.
@misc{etde_6774864,
title = {Cellulolytic properties of an extremely thermophilic anaerobe}
author = {Hudson, J A, Morgan, H W, and Daniel, R M}
abstractNote = {An extremely thermophilic anaerobe was isolated from a New Zealand hot spring by incubating bacterial mat strands in a medium containing xylan. The Gramreaction-negative organism that was subsequently purified had a temperature optimum of 70deg C and a pH optimum of 7.0. The isolate, designated strain H173, grew on a restricted range of carbon sources. In batch culture H173 could degrade Avicel completely when supplied at 5 or 10 g l{sup -1}. There was an initial growth phase, during which a cellulase complex was produced and carbohydrates fermented to form acetic and lactic acids, followed by a phase where cells were not metabolising but the cellulase complex actively converted cellulose to glucose. When co-cultered with strain Rt8.B1, an ethanologenic extreme thermophile, glucose was fermented to ethanol and acetate, and no reducing sugars accumulated in the medium. In pH controlled batch culture H173 produced an increased amount of lactate and acetate but there was again a phase when reducing sugars accumulated in the medium, and these were converted to ethanol by co-culture with Rt8.B1. (orig.).}
doi = {10.1007/BF00604939}
journal = {Applied Microbiology and Biotechnology; (Germany, F.R.)}
volume = {33:6}
journal type = {AC}
place = {Germany}
year = {1990}
month = {Sep}
}