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Increased survival of CBA pluripotent haemopoietic stem cells in vitro induced by a marrow stromal factor in Sl/Sl/sup d/ mice

Journal Article:

Abstract

Media conditioned by marrow adherent cells from anaemic Sl/Sl/sup d/ and W/W/sup v/ mice increased the 24-h survival of CBA CFU/sub s/ in vitro compared to fresh medium to about the same extent as marrow-conditioned medium from normal Sl/sup +//Sl/sup +/, W/sup +//W/sup +/, and CBA mice. Sl/Sl/sup d/ marrow-conditioned medium also increased the percentage of CFU/sub s/ in DNA synthesis to the same extent as CBA marrow-conditioned medium. These results demonstrate that Sl/Sl/sup d/ mice produce a marrow stromal factor that increases both survival of CFU/sub s/ and the percentage of CFU/sub s/ in DNA synthesis in vitro. Therefore, the defective haemopoietic microenvironment of Sl/Sl/sup d/ mice is not due to a deficiency in the production of this factor.
Publication Date:
Jul 01, 1979
Product Type:
Journal Article
Reference Number:
ERA-06-004179; EDB-81-002266
Resource Relation:
Journal Name: Exp. Hematol.; (Denmark); Journal Volume: 7:6
Subject:
59 BASIC BIOLOGICAL SCIENCES; CFU; COMPARATIVE EVALUATIONS; CULTURE MEDIA; STEM CELLS; SURVIVAL TIME; ANEMIAS; BONE MARROW CELLS; DNA; HEMATOPOIETIC SYSTEM; IN VITRO; MICE; SYNTHESIS; ANIMAL CELLS; ANIMALS; BODY; CONNECTIVE TISSUE CELLS; DISEASES; HEMIC DISEASES; MAMMALS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; RODENTS; SOMATIC CELLS; SYMPTOMS; VERTEBRATES; 550300* - Cytology
OSTI ID:
6773987
Research Organizations:
Univ. of California, San Francisco
Country of Origin:
Denmark
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: EXHMA
Submitting Site:
TIC
Size:
Pages: 324-327
Announcement Date:

Journal Article:

Citation Formats

Blackburn, M J, and Patt, H M. Increased survival of CBA pluripotent haemopoietic stem cells in vitro induced by a marrow stromal factor in Sl/Sl/sup d/ mice. Denmark: N. p., 1979. Web.
Blackburn, M J, & Patt, H M. Increased survival of CBA pluripotent haemopoietic stem cells in vitro induced by a marrow stromal factor in Sl/Sl/sup d/ mice. Denmark.
Blackburn, M J, and Patt, H M. 1979. "Increased survival of CBA pluripotent haemopoietic stem cells in vitro induced by a marrow stromal factor in Sl/Sl/sup d/ mice." Denmark.
@misc{etde_6773987,
title = {Increased survival of CBA pluripotent haemopoietic stem cells in vitro induced by a marrow stromal factor in Sl/Sl/sup d/ mice}
author = {Blackburn, M J, and Patt, H M}
abstractNote = {Media conditioned by marrow adherent cells from anaemic Sl/Sl/sup d/ and W/W/sup v/ mice increased the 24-h survival of CBA CFU/sub s/ in vitro compared to fresh medium to about the same extent as marrow-conditioned medium from normal Sl/sup +//Sl/sup +/, W/sup +//W/sup +/, and CBA mice. Sl/Sl/sup d/ marrow-conditioned medium also increased the percentage of CFU/sub s/ in DNA synthesis to the same extent as CBA marrow-conditioned medium. These results demonstrate that Sl/Sl/sup d/ mice produce a marrow stromal factor that increases both survival of CFU/sub s/ and the percentage of CFU/sub s/ in DNA synthesis in vitro. Therefore, the defective haemopoietic microenvironment of Sl/Sl/sup d/ mice is not due to a deficiency in the production of this factor.}
journal = {Exp. Hematol.; (Denmark)}
volume = {7:6}
journal type = {AC}
place = {Denmark}
year = {1979}
month = {Jul}
}