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Molecular cloning and complete nucleotide sequence of a human ventricular myosin light chain 1

Journal Article:

Abstract

Human ventricular plasmid library was constructed. The library was screened with the oligonucleotide probe (17-mer) corresponding to a conserve region of myosin light chain 1 near the carboxy terminal. Full length cDNA recombinant plasmid containing 1100 bp insert was isolated. RNA blot hybridization with this insert detected a message of approximately 1500 bp corresponding to the size of VLCl and mRNA. Complete nucleotide sequence of the coding region was determined in M13 subclones using dideoxy chain termination method. With the isolation of this clone (pCD HLVCl), the publication of the complete nucleotide sequence of HVLCl and the predicted secondary structure of this protein will aid in understanding of the biochemistry of myosin and its function in contraction, the evolution of myosin light genes and the genetic, developmental and physiological regulation of myosin genes.
Publication Date:
Mar 25, 1988
Product Type:
Journal Article
Reference Number:
EDB-88-172199
Resource Relation:
Journal Name: Nucleic Acids Res.; (United Kingdom); Journal Volume: 16:5
Subject:
59 BASIC BIOLOGICAL SCIENCES; MYOSIN; DNA SEQUENCING; DNA-CLONING; BIOLOGICAL EVOLUTION; MAN; MESSENGER-RNA; PROTEINS; ANIMALS; CLONING; GLOBULINS; MAMMALS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PRIMATES; RNA; STRUCTURAL CHEMICAL ANALYSIS; VERTEBRATES; 550200* - Biochemistry
OSTI ID:
6772632
Research Organizations:
Univ. of Toronto, Ontario (Canada)
Country of Origin:
United Kingdom
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: NARHA
Submitting Site:
JMT
Size:
Pages: 2353
Announcement Date:

Journal Article:

Citation Formats

Hoffmann, E, Shi, Q W, Floroff, M, Mickle, D A.G., Wu, T W, Olley, P M, and Jackowski, G. Molecular cloning and complete nucleotide sequence of a human ventricular myosin light chain 1. United Kingdom: N. p., 1988. Web. doi:10.1093/nar/16.5.2353.
Hoffmann, E, Shi, Q W, Floroff, M, Mickle, D A.G., Wu, T W, Olley, P M, & Jackowski, G. Molecular cloning and complete nucleotide sequence of a human ventricular myosin light chain 1. United Kingdom. doi:10.1093/nar/16.5.2353.
Hoffmann, E, Shi, Q W, Floroff, M, Mickle, D A.G., Wu, T W, Olley, P M, and Jackowski, G. 1988. "Molecular cloning and complete nucleotide sequence of a human ventricular myosin light chain 1." United Kingdom. doi:10.1093/nar/16.5.2353. https://www.osti.gov/servlets/purl/10.1093/nar/16.5.2353.
@misc{etde_6772632,
title = {Molecular cloning and complete nucleotide sequence of a human ventricular myosin light chain 1}
author = {Hoffmann, E, Shi, Q W, Floroff, M, Mickle, D A.G., Wu, T W, Olley, P M, and Jackowski, G}
abstractNote = {Human ventricular plasmid library was constructed. The library was screened with the oligonucleotide probe (17-mer) corresponding to a conserve region of myosin light chain 1 near the carboxy terminal. Full length cDNA recombinant plasmid containing 1100 bp insert was isolated. RNA blot hybridization with this insert detected a message of approximately 1500 bp corresponding to the size of VLCl and mRNA. Complete nucleotide sequence of the coding region was determined in M13 subclones using dideoxy chain termination method. With the isolation of this clone (pCD HLVCl), the publication of the complete nucleotide sequence of HVLCl and the predicted secondary structure of this protein will aid in understanding of the biochemistry of myosin and its function in contraction, the evolution of myosin light genes and the genetic, developmental and physiological regulation of myosin genes.}
doi = {10.1093/nar/16.5.2353}
journal = {Nucleic Acids Res.; (United Kingdom)}
volume = {16:5}
journal type = {AC}
place = {United Kingdom}
year = {1988}
month = {Mar}
}