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Immunochemical method for quantitative evaluation of vasogenic brain edema following cold injury of rat brain

Journal Article:

Abstract

An immunochemical method is described for quantitative assessment of serum proteins and hemoglobin content in brain tissue homogenates. Using a combination of affinity chromatography and radioimmunoassay, the sensitivity of the method is 50 ng hemoglobin and 100 ng serum protein per assay, respectively. The method was used to measure cerebral hematocrit, blood volume and serum protein extravasation in rat brain at various times following cold injury. In control rats cerebral blood volume was 6.88 +- 0.15 ml/100 g and cerebral hematocrit 26.4 +- 0.86% (means +- S.E.). Following cold injury blood volume did not significantly change, but there was a gradual increase of extravasated serum proteins, reaching a maximum of 21.54 +- 2.76 mg/g d.w. after 8 hours. Thereafter protein content gradually declined, but even after 64 h it was distinctly increased. Protein extravasation was partly dissociated from the increase of brain water and sodium which reached a maximum already after 2 h and which normalized within 32 and 64 h, respectively. It is concluded that edema fluid associated with cold injury is not simply an ultrafiltrate of blood serum but consists of cytotoxic and vasogenic components which follow a different time course both during formation and resolution of edema.
Authors:
Bodsch, W; Huerter, T; Hossmann, K A [1] 
  1. Max-Planck-Institut fuer Hirnforschung, Koeln (Germany, F.R.). Forschungsstelle fuer Hirnkreislauf-Forschung
Publication Date:
Oct 07, 1982
Product Type:
Journal Article
Reference Number:
AIX-14-719462; EDB-83-052117
Resource Relation:
Journal Name: Brain Res.; (Netherlands); Journal Volume: 249:1
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; 59 BASIC BIOLOGICAL SCIENCES; BRAIN; INJURIES; HEMOGLOBIN; RADIOIMMUNOASSAY; PROTEINS; ANIMAL TISSUES; BIOADSORBENTS; CHROMATOGRAPHY; EDEMA; ELECTROLYTES; QUANTITATIVE CHEMICAL ANALYSIS; QUANTITY RATIO; RATS; WATER; ADSORBENTS; ANIMALS; BODY; CARBOXYLIC ACIDS; CENTRAL NERVOUS SYSTEM; CHEMICAL ANALYSIS; GLOBIN; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; HYDROGEN COMPOUNDS; ISOTOPE APPLICATIONS; MAMMALS; NERVOUS SYSTEM; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; OXYGEN COMPOUNDS; PATHOLOGICAL CHANGES; PIGMENTS; PORPHYRINS; RADIOASSAY; RODENTS; SEPARATION PROCESSES; SYMPTOMS; TISSUES; TRACER TECHNIQUES; VERTEBRATES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics; 550901 - Pathology- Tracer Techniques
OSTI ID:
6766184
Country of Origin:
Netherlands
Language:
English
Other Identifying Numbers:
Journal ID: CODEN: BRREA
Submitting Site:
HEDB
Size:
Pages: 111-121
Announcement Date:

Journal Article:

Citation Formats

Bodsch, W, Huerter, T, and Hossmann, K A. Immunochemical method for quantitative evaluation of vasogenic brain edema following cold injury of rat brain. Netherlands: N. p., 1982. Web.
Bodsch, W, Huerter, T, & Hossmann, K A. Immunochemical method for quantitative evaluation of vasogenic brain edema following cold injury of rat brain. Netherlands.
Bodsch, W, Huerter, T, and Hossmann, K A. 1982. "Immunochemical method for quantitative evaluation of vasogenic brain edema following cold injury of rat brain." Netherlands.
@misc{etde_6766184,
title = {Immunochemical method for quantitative evaluation of vasogenic brain edema following cold injury of rat brain}
author = {Bodsch, W, Huerter, T, and Hossmann, K A}
abstractNote = {An immunochemical method is described for quantitative assessment of serum proteins and hemoglobin content in brain tissue homogenates. Using a combination of affinity chromatography and radioimmunoassay, the sensitivity of the method is 50 ng hemoglobin and 100 ng serum protein per assay, respectively. The method was used to measure cerebral hematocrit, blood volume and serum protein extravasation in rat brain at various times following cold injury. In control rats cerebral blood volume was 6.88 +- 0.15 ml/100 g and cerebral hematocrit 26.4 +- 0.86% (means +- S.E.). Following cold injury blood volume did not significantly change, but there was a gradual increase of extravasated serum proteins, reaching a maximum of 21.54 +- 2.76 mg/g d.w. after 8 hours. Thereafter protein content gradually declined, but even after 64 h it was distinctly increased. Protein extravasation was partly dissociated from the increase of brain water and sodium which reached a maximum already after 2 h and which normalized within 32 and 64 h, respectively. It is concluded that edema fluid associated with cold injury is not simply an ultrafiltrate of blood serum but consists of cytotoxic and vasogenic components which follow a different time course both during formation and resolution of edema.}
journal = {Brain Res.; (Netherlands)}
volume = {249:1}
journal type = {AC}
place = {Netherlands}
year = {1982}
month = {Oct}
}